胞外植酸酶高产酵母菌的选育

从酒糟样品中筛选得到五株胞外植酸酶活性较高的酵母，选择酶活最高的１－１作为出发菌株，经单倍体分离、原生质体制备、紫外诱变，最后得到一株突变株Ｍｓｐ－２１２８，其胞外植酸酶活力为２３．０８Ｕ／ｍＬ，比出发菌株提高了８２％，且产酶稳定     

胞外植酸酶产酵母菌的选育

从酒糟样品中筛选得到五株胞外植酸酶活性较高的酵母，选择酶活最高的１－１作为出发菌株，经单倍体分离，原生质体制备，紫外诱变，最后得到一株突变株Ｍｓｐ－２１２８，其胞外植酸酶活力为２３．０８Ｕ／ｍｌ，比出发菌株提高了８２％，且产酶稳定。     

植酸酶高活性菌株的选育及酶性质的初步研究

以本实验室筛选的一株米曲霉为出发菌株，通过紫外线诱变，并经过初筛、复筛等步骤而得到一株植酸酶高产菌株，酶活约提高１５６％，最高酶活达２４０Ｕ／ｍｌ粗酶液，７２ｈ左右达到产酶高峰期．植酸酶的最适温度为５６℃，最适ｐＨ为５．５；Ｆｅ２＋、Ｚｎ２＋、Ａｌ３＋对其酶活有强烈的抑制作用，而Ｍｇ２＋和ＥＤＴＡ在浓度为１ｍｍｏｌ／Ｌ时有激活作用     

复合诱变及发酵优化选育染料木素转化菌株的研究

本研究通过对两株β-葡萄糖苷酶高产菌株黄丝曲霉JS-Q和黑曲霉XK-L进行复合诱变,诱变混合菌株直接进行产酶培养,利用"复杂系统定向调控技术",以酶活为指标对培养条件进行连续的定向调控,在产酶培养条件优化过程中富集正突变株并逐步提高酶活,在挑选出正突变株的同时优化出适合高酶活正突变株的培养条件.最终从优化产酶培养基中挑选到菌株F17和F27,产酶能力比出发前菌株XK-L提高了64.9%和49.4%.用这两株菌分别发酵槐角苷,经测定其转化率分别为76%和78%,比出发菌株XK-L提高31.0%和34.5%.     

胡萝卜素产生菌原生质体诱变育种的研究

采用原生质体制备技术与单一因素多次诱变育种相结合的育种技术，对产胡萝卜素酵母原生质体进行诱变处理，选育出一株比亲株胡萝卜素产量高１．６倍的新菌株     

激光复合诱变黑曲霉原生质体选育热稳定菊粉酶高产菌

以菊粉酶产生菌黑曲霉ＡＳ－４原生质体为对象,经激光和亚硝基胍复合诱变,从大量突变株中最终选育出高产热稳定的菊粉酶产生菌ＡＬ－１５４．在以菊芋提取液为碳源、玉米浆加０．３％酵母膏为氮源的基本培养基中,菊粉酶活力达１４６．３ｕ／ｍＬ,比出发株提高近３倍．其产酶最适营养条件为：起始ｐＨ４．５～５．０,２８～３０℃,３６ｈ时产酶量达最高．酶作用最适条件为ｐＨ５．０,６０℃;该酶遗传性能稳定,热稳定性强,６０℃保温处理２ｈ,其酶活性不变,６５℃保温３０ｍｉｎ,仍保留９８％的初始酶活     

产植酸酶青霉菌株的诱变研究

对产植酸酶的青霉菌株Ａ２进行了诱变研究。分别以ＨＮＯ２－ＵＶ和ＵＶ－５Ｂｕ复合处理，进行了二轮诱变筛选后，得到高于Ａ２５０％以上的变异株６株，其中Ａ２－ＵＶ５９－２０株的酶活达０．８１２±０．０１９ｕ／ｍｌ，为出发菌株Ａ２的二倍多。     

植酸酶高活性菌株的选育及酶性质的初步研究

以本实验室筛选的一株米曲霉为出发菌株，通过紫外线诱变，并经过初筛、复利筛等步骤得到一株植酸酶高产菌株，酶活约提高１５６％，最高酶活达２４０Ｕ／ｍｌ粗酶液，７２ｈ左右达产酶高峰期。     

黑曲霉(Aspergillus niger)原生质体电融会(Ⅰ)─黑曲霉原生质体形成与再生的研究

通过紫外诱变获得两株稳定的黑曲霉营养缺陷型Ｍｕ２（Ｍｅｔ－）和Ｄ１２（Ｃｙｓ－），并首次证实低温黑暗保存数小时对黑曲霉突变株具有稳定作用。系统地研究了菌龄、渗透压稳定剂、温度、酶的浓度和酶解系统等因素对黑曲霉原生质体形成的影响．采用２％溶壁酶加０．５％纤维素酶，获得了大量的原生质体。在显微镜下观察原生质体形成和再生过程， Ｍｕ２和 Ｄ１２再生率分别为 ５４％和 ３３％。原生质体在正弦交变电场（１ＭＨｚ，４５０ Ｖ／ｃｍ）作用下形成珠串，在高压电脉冲（强度 ９．０ ｋＶ／ｃｍ、时程 ２５ μｓ）触发下，发生融合。     

产植酸酶菌株的筛选、诱变及酶学性质初探

从富含有机质的环境中采集土样,通过植酸钙平板透明圈法初筛和维生素C-硫酸-钼酸铵-酒石酸锑钾法测定植酸酶酶活复筛,共筛选到5株产植酸酶的霉菌,对这5株菌株进行紫外线和亚硝基胍复合诱变处理,最终获得一株高产植酸酶突变株NTG-506,酶活达到112 U/mL,比原始出发菌株的酶活提高了3.2倍.突变株NTG-506在250mL三角瓶中发酵培养,在装液量60 mL,接种量2%时,培养4 d后酶活达到最高,为124.5 U/mL.植酸酶的最适反应温度35℃,经50℃处理10 min后剩余酶活为原来的57%.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with