红霉素与牛血清白蛋白的相互作用机制

利用荧光及紫外光谱法研究了水溶液体系中红霉素与牛血清白蛋白(BSA)的相互作用机制.结果表明红霉素对牛血清白蛋白的荧光有较强的猝灭作用,其猝灭类型主要为静态猝灭.在不同温度下求得了红霉素与牛血清白蛋白的结合常数K,发现随反应温度上升K值下降.由热力学参数确定了红霉素与牛血清白蛋白的结合作用主要为范德华力.又根据Frster理论,测得了红霉素与牛血清白蛋白结合的能量转移效率,相互结合距离和结合位点.进一步证明了该反应是单一静态猝灭过程,阐述了其猝灭机理是通过能量转移产生的.     

一种新型Schiff碱的合成及其与牛血清白蛋白的相互作用

合成了1种新型具有良好生物活性的4,5-二甲基-3-腈基-2-呋喃胺对苯二甲醛Schiff碱(FTS).并通过红外和氢核磁共振谱对FTS的结构进行表征.应用荧光光谱法、紫外光谱法研究了该化合物与牛血清白蛋白(BSA)之间在不同温度下的相互作用.实验表明,FTS能强烈猝灭BSA的内源荧光,猝灭机理为静态猝灭.在此基础上计算了二者相互作用的结合常数、结合位点数及热力学参数等,结果表明,FTS与BSA分子以摩尔比1∶1结合,其结合反应主要是熵驱动,主要作用力是疏水力.同时,应用同步荧光考察了FTS对BSA构象的影响.     

阿昔洛韦与牛血清蛋白相互作用研究

目的研究了阿昔洛韦(ACV)与牛血清白蛋白(BSA)之间的相互作用。方法在pH为7.4的Tris-HCl缓冲液中,发现阿昔洛韦在348 nm(λex=282 nm)对牛血清白蛋白的荧光有猝灭作用。并对阿昔洛韦对牛血清白蛋白荧光猝灭的机理进行了初步探讨。结果发现在弱酸性、中性及弱碱性条件下均表现为静态猝灭。利用荧光猝灭双倒数图计算了阿昔洛韦与牛血清白蛋白之间的表观结合常数为1.48×10~4、结合位点数为1,结合位置接近于色氨酸残基,牛血清白蛋白中色氨酸残基与阿昔洛韦分子间的距离为3.138 nm。结论根据热力学参数计算确定了阿昔洛韦与牛血清白蛋白之间的主要作用力类型为疏水作用力,同时,采用同步荧光技术考察了阿昔洛韦对牛血清白蛋白构象的影响。     

荧光法研究阿托伐他汀钙与牛血清白蛋白的相互作用

在不同温度下测定了阿托伐他汀钙(AC)对牛血清白蛋白(BSA)的荧光光谱的影响.结果表明,AC会使BSA发生荧光猝灭,随着AC浓度的增大,猝灭程度增强.AC对BSA的猝灭作用主要是动态猝灭,并计算得到AC与BSA的结合位点数为1.2.根据热力学方程式得出AC与BSA相互作用的参数,说明AC与BSA之间的相互作用力主要是疏水作用力.     

荧光法研究阿托伐他汀钙与牛血清白蛋白的相互作用

在不同温度下测定了阿托伐他汀钙(AC)对牛血清白蛋白(BSA)的荧光光谱的影响.结果表明,AC会使BSA发生荧光猝灭,随着AC浓度的增大,猝灭程度增强.AC对BSA的猝灭作用主要是动态猝灭,并计算得到AC与BSA的结合位点数为1.2.根据热力学方程式得出AC与BSA相互作用的参数,说明AC与BSA之间的相互作用力主要是疏水作用力.     

N,N-双(2-羟基-5-氯苄基)-正丁胺与牛血清白蛋白相互作用的研究

在不同温度下,研究了N,N-双(2-羟基-5-氯苄基)-正丁胺(HN)作用于牛血清白蛋白(BSA)的荧光猝灭光谱,同步荧光光谱.结果表明:HN可以形成HN-BSA复合物,还可以对牛血清白蛋白的荧光强度进行强烈猝灭,它的荧光猝灭的机理是动态猝灭.在此基础上计算了二者相互作用的结合常数、结合位点数及ΔH,ΔG,ΔS等热力学参数.结果表明HN与BSA以1:1结合,其反应主要是熵驱动的疏水作用力.     

采用荧光猝灭法研究穗花杉双黄酮与牛血清白蛋白的相互作用

目的:运用荧光猝灭法研究不同温度下穗花杉双黄酮(AMF)与牛血清白蛋白(BSA)的相互作用.方法:采用荧光光谱研究AMF与BSA之间的猝灭类型,计算二者之间的结合常数及结合位点数等.结果:穗花衫双黄酮能使BSA发生内源性荧光猝灭,属静态猝灭机制.在298,308,318 K下,AMF与BSA结合常数分别为6.73,6.38,6.17 L·mol-1,结合位点数n近似为1;热力学分析表明AMF与BSA之间结合力为静电力作用;同步荧光光谱表明AMF使BSA构象发生改变,且色氨酸所处微环境的疏水性增强.结论:荧光猝灭法可用于AMF与BSA之间结合反应的研究,方法简便,灵敏度高.     

光谱法研究一种含咪唑啉酮类衍生物与牛血清白蛋白的相互作用

应用荧光光谱及紫外可见光谱的方法研究了2-氨基-5-苯亚甲基-4H-咪唑啉-4-酮衍生物(BPH5)与牛血清白蛋白(BSA)的相互作用.实验发现BPH5能强烈猝灭牛血清白蛋白的荧光强度,其荧光猝灭机理为动态猝灭.在此基础上计算了二者相互作用的结合常数、结合位点数及△H,△G,△S等热力学参数等.结果表明BPH5与BSA以1∶1结合,其反应主要是熵驱动的,相互作用力主要为疏水作用力.根据F(o)rster无辐射能量转移理论计算了给体(BSA)与受体(BPH5)之间的结合距离.     

冬凌草丁素与牛血清白蛋白的相互作用

利用荧光光谱法和紫外吸收光谱法研究了冬凌草丁素与牛血清白蛋白(BSA)的相互作用机制,确定冬凌草丁素对BSA荧光的猝灭主要是静态猝灭过程,依据所得结果测得不同温度下冬凌草丁素与BSA的结合常数、结合距离和能量转移效率.     

4,5-二甲基-3-腈基-2-呋喃胺水杨醛Schiff碱的合成及其与牛血清白蛋白的相互作用

合成了一种新型具有良好生物活性的4,5-二甲基-3-腈基-2-呋喃胺水杨醛Schiff碱(FSS),通过核磁共振、红外光谱及质谱等对其结构进行了表征;应用荧光光谱及紫外可见光谱法研究了该化合物(FSS)与牛血清白蛋白(BSA)之间在不同温度下的相互作用.实验表明,FSS能强烈猝灭BSA的内源荧光,猝灭机理为动态猝灭.在此基础上计算了二者相互作用的结合常数、结合位点数及热力学参数等.结果表明,FSS与BSA分子以物质的量比1∶1结合,其结合反应主要是熵驱动,主要作用力是疏水力.同时,应用同步荧光考察了FSS对BSA构象的影响。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

正~~     

Brief Introduction to “Journal of Jilin University( Science Edition) ”

<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has