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1.
T. Matsuoka T. Nishizaki Y. Ikeuchi Y. Okada K. Sumino 《Cellular and molecular life sciences : CMLS》1997,53(3):233-236
Effects of serotonin (5-HT) on cerebral cortical neurons were examined by patch clamp techniques. 5-HT produced a variety
of responses such as outward (19/73 patches/neurons), slow inward (15/73 patches/neurons), fast inward (8/73 patches/neurons),
and mixed currents (initially fast inward deflection followed by an outward response: 2/73 patches/neurons), with a latency
of 12 sec, 15 sec, 0 sec, and 0 sec respectively, at a holding potential of −60 mV in whole-cell patches. The fast inward
currents were again evoked by a selective 5-HT3 receptor agonist, 1-(m-chlorophenyl)-biguanide hydrochloride (CPBG). In the
cell-attached patch clamp configuration, 5-HT inside the patch pipette elicited single channel currents with slope conductances
of 42 pS and 132 pS (4/42 patches/neurons). CPBG inside the patch pipette evoked inward single channel currents with a lower
slope conductance of 41 pS (3/23 patches/neurons). In contrast, application of 5-HT or a 5-HT2 receptor agonist, α-methyl-5-hydroxytryptamine-maleate, outside the patch pipette induced outward single channel currents with a major slope
conductance of 140 pS (8/30 patches/neurons) or 135 pS (6/20 patches/neurons), respectively. These results indicate that the
outward and fast inward currents may be mediated respectively by the 5-HT2 receptor, which is coupled to a G-protein, and by the 5-HT3 receptor, which contains the non-selective cation channel, and that the mixed type may be caused by both the 5-HT2 and 5-HT3 receptors.
Received 27 September 1996; received after revision 4 November 1996; accepted 7 November 1996 相似文献
2.
Neurodegeneration changes in primary central nervous system neurons transfected with the Alzheimer-associated neuronal thread protein gene 总被引:6,自引:0,他引:6
The AD7c-NTP gene is over-expressed in brains with Alzheimer's disease (AD), and increased levels of the corresponding protein
are detectable in cortical neurons, brain tissue extracts, cerebrospinal fluid, and urine beginning early in the course of
AD neurodegeneration. In the present study, we utilized a novel method to transfect post-mitotic primary neuronal cell cultures,
and demonstrated that over-expression of the AD7c-NTP gene causes cell death and neuritic sprouting, two prominent abnormalities
associated with AD. These results provide further evidence that aberrantly increas-ed AD7c-NTP expression may have a role
in AD-type neurodegeneration. In addition, we demonstrate that primary post-mitotic neurons can be efficiently transfected
with conventional recombinant plasmid DNA to evaluate the effects of gene over-expression in relevant in vitro models.
Received 31 January 2001; received after revision 31 March 2001; accepted 4 April 2001 相似文献