鹿茸醇提物抗氧化作用的实验研究

用鹿茸醇提物对用环磷酰胺处理后的小鼠进行灌胃实验,以探讨鹿茸醇提物对该小鼠抗氧化作用的影响。实验结果表明:鹿茸醇提物组(即实验组)小鼠每50μL血中的红细胞内SOD的活性为191±07u,而对照组为152±09u,两组相比较,差异显著(P<0.05);实验组小鼠肾脏中丙二醛(MDA)的含量为327±02nmol/g,而对照组为356±05nmol/g,两组相比较,差异显著(P<0.05)。结果提示,鹿茸醇提物可增强该小鼠的抗氧化作用     

SLE患者淋巴细胞凋亡及血清新蝶呤、γ-干扰素水平研究

探讨SLE患者体内淋巴细胞凋亡率、巨噬细胞功能相关的细胞因子(新蝶呤、γ-干扰素)水平及两者间相互关系,并同时与抗ds-DNA抗体滴度、疾病活动性评分(SLAM评分)进行相关性分析.应用Annexin V凋亡检测试剂盒及流式细胞仪检测淋巴细胞凋亡率;采用ELISA法检测血清γ-干扰素、新蝶呤水平.结果发现:(1)SLE活动期患者外周血淋巴细胞凋亡率((13.07±7.39),n=30)明显高于非活动期患者((4.08±3.55),n=8,P<0.001)及正常人((5.13±3.37),n=11,P<0.001).(2)抗ds-DNA抗体阳性组患者淋巴细胞凋亡率((12.98±9.25),n=20)与阴性组((9.35±4.76),n=18)相比无差异(P=0.14).淋巴细胞凋亡率与抗ds-DNA抗体滴度无相关性(r=0.112,P=0.77).(3)SLE患者血清新蝶呤水平((1.39±1.10)μg/L,n=20)极显著高于正常人((0.36±0.19)μg/L,n=20,P<0.01).(4)SLE活动期患者血清γ-干扰素水平((58.97±34.52)ng/L,n=15)显著高于正常人((28.06±2.35)ng/L,n=16,P<0.05).(5)SLE患者淋巴细胞凋亡率与血清新蝶呤水平呈正相关(r=0.446,P<0.05,n=22),与SLAM评分呈正相关(r=0.533,P<0.001,n=38),血清新蝶呤水平与SLAM评分亦呈正相关(r=0.485,P<0.05,n=22).未发现SLE患者淋巴细胞凋亡与抗ds-DNA抗体产生相关,未发现明显升高的细胞凋亡率与血清新蝶呤     

胎膜早破对新生儿脐血免疫球蛋白及补体的影响

目的:研究胎膜早破时新生儿脐血免疫球蛋白、补体的变化规律。方法:应用速率透射比浊法检测79例新生儿脐血IgG、IgA、IgM和补体C3、C4水平,追踪新生儿出生1周内感染发生的情况,其中有胎膜早破史的研究组38例,无胎膜早破史的对照组41例。结果:胎膜早破组脐血IgA(0.16±0.12)g/L,对照组(0.06±0.03)g/L,(P<0.01);胎膜早破组IgM(0.24±0.15)g/L,对照组(0.16±0.03)g/L,(P<0.01),差异有统计学意义;脐血IgG水平无明显变化;脐血IgA浓度在胎膜早破72h为0.234±0.156)g/L,较胎膜早破<12h的0.091±0.047g/L明显增加,差异有统计学意义(P<0.05);脐血补体C3(0.76±0.35)g/L,对照组(0.93±0.21)g/L,(P<0.05),C4(0.16±0.09)g/L,对照组0.20±0.05g/L,(P<0.05),研究组明显低于对照组,差异有统计学意义。结论:有胎膜早破史的新生儿,脐血免疫球蛋白IgA、IgM浓度升高,IgG不受胎膜早破的影响,脐血补体C3、C4水平下降。脐血IgA、IgM及补体C3、C4均能反映胎膜早破个体合并宫内感染。     

力竭性运动对大鼠骨骼肌Ca2+含量的影响

观察大鼠力竭性运动后即刻组及三小时后组(以下简称3h组)骨骼肌内Ca2+的变化情况,探讨骨骼肌Ca2+在力竭性运动后和恢复中的变化及其机制.力竭性运动后取大鼠骨骼肌,采用原子吸收分光光度计测其Ca2+浓度.运动后即刻组骨骼肌中Ca2+浓度(2068.33±752.02μg/g)比对照组(615.31±546.03μg/g)有极显著增高(P＜0.001),3 h组(734.58±258.70μg/g)与对照组无明显差异,即刻组和3 h组有极显著性差异(P＜0.001)力竭性运动即刻,骨骼肌中Ca2+浓度显著增高,恢复期内Ca2+浓度逐渐恢复.     

放牧条件下牦牛泌乳若干生理特性的研究

对青草季节放牧条件下78头四川麦洼牦牛泌乳生理特性作了研究.麦洼牦牛需有犊牛的吮乳刺激才能引起排乳反射,一般为两期,全奶牦牛潜伏期分别为48.8±14.1和56.1±15.1秒,差异极显著(P<0.01).Ⅱ期乳脂率和乳脂小球数极显著高于Ⅰ期.全奶牦牛的日挤乳量为1324±473ml,Ⅰ期占73.8%.泌乳一年以上的半奶牦牛有些Ⅰ期即可将乳排尽,排乳反射的潜伏期明显短于全奶牦牛,日挤乳量为576±37lml,Ⅰ期占83.7%.半奶牦牛的乳蛋白和乳脂含量均高于全奶牦牛(P<0.01).     

碱性成纤维细胞生长调节因子对家兔实验性深Ⅱ度烫伤的治疗作用

为了探讨碱性成纤维细胞生长调节因子(bFGF)对家兔实验性深Ⅱ度烫伤的治疗作用。治疗组采用bFGF 10μg/ml、3μg/ml、1μg/ml的3种浓度;对照组采用溶媒、磺胺嘧啶银盐、生理盐水。观察bFGF对家兔被80℃循环水所致深Ⅱ度烫伤的促愈合作用。结果表明,3种不同浓度的bFGF对家兔深Ⅱ度烫伤均具有促进烫区表面干燥,结痂和脱痂的作用。t检验的结果表明,在缩小痂皮面积方面各治疗组于涂药前后不同时点的对比,差并非常显著(P<0.01),但各对照组仅在第25天开始差异显著或非常显著(P<0.05或P<0.01);而痂皮脱落时间与各对照组相比10μg/ml组差异显著(P<0.05),3μg/ml、1μg/ml组脱痂时间除与生理盐水组相比未显示统计学意义外,其余均具有统计学显著性(P<0.05)。     

半胱胺对鹅生长内分泌的影响

探讨了半胱胺(CS)对成年鹅血浆中生长抑素(SS)、生长激素(GH)和胰岛素样生长因子-I(IGF-I)的影响和调节机理.14只装有翅静脉瘘管的成年杂交鹅(川白×太湖),自身对照.试验期于日粮中一次性添喂半胱胺(100mg/kg,按体重计),自由采食和饮水.采取对照期和处理后第1、3、5、7d的血样,用RIA双抗法测定其中激素的含量.结果表明,试验期SS较对照期(1.89±0.10)μg/L分别降低了41.18%(P<0.01)、26.74%(P<0.01)、37.43%(P<0.01)和17.11%(P<0.05);试验期第1、3、5和7d的GH较对照期(0.55±0.15)μg/L显著(P<0.01)升高,分别为34.55%、78.18%、65.45%和54.55%;试验期的IGF-I较对照期(25.29±4.91)μg/L分别升高了7.28%、59.07%(P<0.01)、22.41%(P<0.05)和1.38%.因此,CS能够降低成年鹅血液中SS含量,使GH和IGF-I水平升高,从而调节鹅的神经内分泌,促进生长.     

大鼠肝刺激因子对CCl_4致肝损伤小鼠保护作用的机制研究——Ⅰ.HSS的抗氧化作用

本工作观察了大鼠肝刺激因子(HSS)对CCl_4损伤小鼠后,肝组织和血清脂质过氧化物丙二醛(MDA)及自由基清除剂谷胱甘肽(GSH)含量的影响。结果发现,HSS(0.1mg/g体重,i p)可使CCl_4(10％,5ml/kg)中毒后48h,肝组织MDA值的升高幅度明显降低(6.23±0.98nmol/mg蛋白vs.4.17±1.67nmol/mg蛋白,P<0.005),但血清MDA值的下降幅度不明显。同时肝组织GSH含量明显高于CCl_4损伤组(3236±472μg/g vs.2007±589μg/g,P<0.001)。上述结果提示,HSS具有抗氧化作用,降低CCl_4自由基对肝细胞膜的损伤作用,保护受损肝脏。     

中草药保健剂“鸡食乐”对雏鸡红细胞免疫功能的影响研究

本研究将健康1日龄京白雏鸡100只随机均分为试验组和对照组.试验组饮水中添加“鸡食乐”煎汁,使饮水药液浓度为1%让鸡自由饮用;对照组饮水中不添加任何药物.饲养至7周龄,各组取15只采血测试红细胞免疫功能.结果,试验组、对照组红细胞C_(3b)受体花环形成率(E-C_(3b)R)分别为13.4±1.51%、10.6±1.25%,组间差异极显著(P<0.01),红细胞免疫复合物花环形成率(E—IC)分别为5.4±1.51%、4.7±1.34%,组间差异不显著(P>0.05),红细胞C_(3b)受体花环促进率(RFER)分别为55.5±10.8%、38.3±10.7%,组间差异极显著(P<0.01);红细胞C_(3b)受体花环抑制(RFIR)分别为17.4±3.4%、17.7±3.8%,组间差异不显著(P>0.05).     

尼玛县绒山羊的生长发育和产绒性能

经测定尼玛县藏山羊的生长发育和产绒性能,研究结果:羔羊初生重公羔为2.07±0.19kg,母羔为1.99±0.15kg, 公羔比母羔重约0.08kg,差异不显著(P>0.05);断奶重公羔为8 34±0.94kg,母羔为8.13±0.98kg,公羔比母羔重0.21kg, 差异极显著(P<0.01);周岁体重公羊为19.22±2.62kg,母羊为15.19±1.85kg;2岁体重公羊为28.11±4.09kg,母羊为 20.36±2.62kg;3岁以上公羊为36.66±5.89kg,母羊为23.31±2.61kg,周岁后体重在公羊与母羊间差异极显著(P<0.01);周 岁、2岁和3岁以上的体尺随年龄的增长而增大;产绒量周岁公羊为187.70±54.55g,母羊为175.61±33.90g,2岁公羊为 209.10±41.99g,母羊为191.10±31.20g,3岁以上公羊为324.00±80.20g,母羊为286.10±60.20g,产绒量在性别间差异极显 著(P<0.01),产绒量随着年龄的增长逐渐增加;绒层高度在年龄间差异不显著(P>0.05);在3岁以后生长发育和产绒性能 均趋于稳定.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that