人芽囊原虫扫描电镜观察

扫描电镜观察,人芽囊原虫空泡型虫体的表面较光滑;阿米巴型虫体表面高低不平,呈锯齿状和块状突起;颗粒型虫体表面呈长条形内陷。颗粒型虫体表面的颗粒是在细胞质中产生的,细胞外膜破裂后露丁体表,脱离表面后,表面留有半球形凹陷。颗粒型虫体内和表面的圆形颗粒性质一致,为线粒体、微小体等结构,纤丝层在空泡型虫体中可能起着维持虫体形,增强虫体表面强度的作用,老化的空泡型虫体失去了上述功能;在阿米巴型虫体中,起着非专一性粘着细菌的功能。本文认为人芽囊原虫细胞外环境与细胞内环境之间有潜在通道。     

营养物失衡是维持癌细胞恶性生长的因素之一--DMEM培养基使黑色素瘤B16细胞的恶性生长抑制

对小鼠黑色素瘤B16细胞在DMEM和RPMI1640两种培养基中培养产生的活细胞数和黑色素量进行了检测.结果显示,该癌细胞在DMEM培养基中培养产生的活细胞数比在RPMI1640培养基中培养产生的活细胞数平均减少87.9%,而黑色素量平均增加795%.以"黑色素量/活细胞数"计算分化指数,再以"DMEM中的分化指数/1640中的分化指数"计算DMEM相对于1640的对B16细胞的分化指数,显示DMEM中的分化指数平均是1640中的分化指数的67.8倍.提出营养物失衡是维持癌细胞恶性生长的因素之一.     

云南部分地区黑山羊的芽囊原虫分子检测及基因分型

目的芽囊原虫(Blastocystis)是一种重要的人和动物消化道寄生虫。主要引起腹泻、腹痛及消瘦等疾病。其流行呈世界性分布,且发展中国家有更高的感染率。至今已确定的17种芽囊原虫基因亚型(ST1-17),其中ST1-9属于人兽共患型,ST10-17截至目前只在动物体内所检测到。方法本研究就在云南5个地区采集到的907份样品,基于SSU rRNA序列通过分子生物学方法检测,芽囊原虫总体检出率为41. 3%(375/907)。结果共鉴定出3个已知基因亚型(ST5,ST10,ST14)和4个新亚型(ST-novel 1,ST-novel 2,ST-novel 3,ST-novel 4),其中ST5作为该地区的芽囊原虫优势基因亚型。本试验还通过统计学方法分析其在年龄、性别和地区间均存在显著性差异。结论本研究不仅为云南地区黑山羊芽囊原虫防控提供了数据支撑,也进一步评估当地的公共卫生安全风险,具有重要公共卫生意义。     

营养物失衡是维持癌细胞恶性长的因素之一—DMEM培养基使黑色素瘤B16细胞的恶性生长抑制

对小鼠黑色素瘤B16细胞在DMEM和RPMI1640两种培养基中培养产生的活细胞数和黑色素量进行了检测。结果显示，该癌细胞在DMEM培养基中培养产生的活细胞数比在RPMI1640培养基中培养产生的活细胞数平均减少87.9%，而黑色素量平均增加795%，以“黑色素量/活细胞数”计算分化指数，再以“DMEM中的分化指数/1640中的分化指数”计算DMEM相对于1640的对B16细胞的分化指数，显示DMEM中的分化指数平均是1640中的分化指数的67.8倍。提出营养物失衡是维持癌细胞恶性生长的因素之一。     

营养环境对T细胞中IL-2表达影响的初步研究

白细胞介素2(IL-2)是一种重要的细胞因子,在机体免疫应答中发挥多种功能.探明不同营养环境对IL-2的影响对于细胞免疫应答、活化等基础研究均具有重要意义.通过使用RPMI、IMDM两种不同的细胞培养基,对小鼠淋巴细胞进行培养,分析T细胞中IL-2的表达水平.结果显示,IMDM培养基条件下,CD4 + 和CD8 + T细胞中IL-2的表达均高于RPMI培养基,且IL-2的表达呈先下降后上升的动态改变.IMDM培养条件下,T细胞的细胞增殖能力与RPMI培养基类似.RPMI中增加氨基酸含量不能上调T细胞中IL-2的表达.研究结果为深入探讨外界环境对细胞分化和功能影响的研究奠定了初步的基础.     

兰州百合的组织培养

以兰州百合鳞片作为外植体进行组织培养,分别对其进行芽诱导、增殖、生根培养,得到了兰州百合鳞片组织培养的最佳培养基配方：（1）芽诱导培养基：MS＋BA0．6mg／L＋NAA0．2mg／L＋琼脂7．3g／L＋蔗糖30g／L,pH5．8;（2）增殖培养基：Ms＋BA0．8mg／L＋NAA0．05mg／L＋琼脂7．3g／L＋蔗糖30g／L,pH5．8;（3）生根培养基：MS＋BA0．05mg／L＋NAA0．8mg／L＋琼脂7．3g／L＋蔗糖30g／L,pH5．8．观察与分析结果表明,外植体在前两种培养基上的繁殖速率较前人快约15d,第3种培养基与前人的相当,比其快2d．     

兰州百合的组织培养

以兰州百合鳞片作为外植体进行组织培养,分别对其进行芽诱导、增殖、生根培养,得到了兰州百合鳞片组织培养的最佳培养基配方：（1）芽诱导培养基：MS＋BA0．6mg／L＋NAA0．2mg／L＋琼脂7．3g／L＋蔗糖30g／L,pH5．8;（2）增殖培养基：Ms＋BA0．8mg／L＋NAA0．05mg／L＋琼脂7．3g／L＋蔗糖30g／L,pH5．8;（3）生根培养基：MS＋BA0．05mg／L＋NAA0．8mg／L＋琼脂7．3g／L＋蔗糖30g／L,pH5．8．观察与分析结果表明,外植体在前两种培养基上的繁殖速率较前人快约15d,第3种培养基与前人的相当,比其快2d．     

仙客来(Cyclaman Persicum,Mill)组织培养中不定芽形态发生的组织细胞学研究

分别用仙客来种苗的子叶和叶柄作为外植体,培养在附加2,4 D、细胞分裂素和细胞激动素的1/2MS培养基上诱导不定芽的发生,研究了愈伤组织和不定芽形成过程中的组织细胞学变化.对两种不同外植体不定芽发生过程中的切片观察表明,两种外植体的组织脱分化始于维管束周围的维管束鞘细胞,随后开始分裂的是与其临近的薄壁细胞并能很快形成胚性分生细胞团.经30d左右的培养,进一步分化形成芽原基.子叶愈伤的芽原基通常出现在愈伤的边缘.叶柄脱分化比子叶快,不定芽可以起源于表层的分生细胞团,也可以由愈伤深处的分生组织分化而形成.     

不同湍流模型在90°弯管数值模拟中的应用

为了揭示不同湍流模型对弯管内部流动模拟的影响因素,在90°弯管PIV(粒子成像测速仪)试验研究的基础之上,采用6种湍流模式模拟了方形截面90°弯管内的三维湍流流动,与试验数据进行了比较,并根据试验与数值计算的比较结果,选择较优的湍流模型模拟了半径r=164mm的速度分布曲线以及各个截面内的流动情况.结果表明:LES(大涡模拟)模型的计算结果在流体运动的整个过程中与试验值符合较好;其他湍流模型模拟弯管直线段时,计算误差较小,而一旦流体进入弯曲段后,计算值与试验值存在一定差异.相对于RNG(重正化群)k-ε(湍动能-耗散率)湍流模型,LES模型计算半径r=164mm上速度分布与试验值更为接近.此外,LES模型能够较好地模拟出弯管各个断面上的二次流动情况.     

啤酒酵母(Saccharomy cescerevisiae)变株M-05合成谷胱甘肽的研究:(II)发酵条件的研究

以谷胱甘肽 ( GSH)产生菌啤酒酵母甲硫氨酸缺陷型变株 M-0 5为试验菌株 ,进行培养基、培养条件的初步研究 .经培养基、培养条件的优化 ,M-0 5胞内积累 GSH比原来配方提高了 61 .3% ,培养基中添加适当甲硫氨酸 ( Met) ,则胞内 GSH含量提高了 1 .2倍 ,达 32 .95mg/ g     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that