Common antigenic properties of a g-type (goose) and a c-type (duck) egg white lysozyme: Antibody responses in rabbits and mice

Embden goose (GEWL) and Barbary duck (DEWL) egg white lysozymes possess different amino acid sequences corresponding to the g-type and c-type, respectively. GEWL was shown to be a better immunogen than DEWL in both rabbits and mice. The antigenicity of the two lysozymes was tested using different technique (i.e. indirect ELISA, inhibition tests and immunoabsorption experiments). Injection of either GEWL or DEWL into rabbits and mice induced both specific antibodies and cross-reacting antibodies. Moreover, anti-GEWL antibodies, in contrast to anti-DEWL antibodies, did not cross-react with hen egg white lysozyme (HEWL), a c-type lysozyme. While the structure of GEWL was not modified after binding to plastic, DEWL was denaturated, but it did keep some native epitopes. It was concluded that g-type and c-type lysozymes, which have different amino acid sequences, exhibit strong common antigenic properties.     

Enzymatic activities of muscle fibres differentiated,in vitro,from pectoralis major (white) and adductor magnus (red) muscles of chick embryos

Summary Specific activities of NADP isocitrate dehydrogenase and acetylcholinesterase were significantly higher in muscle fibres differentiated, in vitro, from myoblasts of adductor magnus (red) than pectoralis major (white) muscles 10-day-old chick embryos. This is evidence, as far as enzyme activities, are concerned, that myoblasts from different types of skeletal muscles are able to give, in tissue culture, muscle fibres of different properties, even in the absence of nerve supply.Supported by a grant No. 75 7 1641 from the French Délégation à la Recherche scientifique et technique.     

Gamma-glutamyl-transpeptidase in lymphatic tissues of Mastomys natalensis during an infection with Acanthocheilonema viteae

During Acanthocheilonema viteae infection, the specific activity of gamma-glutamyltranspeptidase (gamma-GT) increased in peritoneal exudate cells and bone marrow and decreased in lymph nodes of Mastomys natalensis throughout the course of infection. However, though there was an increase in specific activity of gamma-GT in thymus and spleen during the prepatent phase of A. viteae infection, the level either returned to normal or decreased during the latent phase of infection. A close correlation was observed between the host's immune status during A. viteae infection and the level of gamma-GT in lymphoid tissues.     

An immunologically detectable change in the egg white of chicken eggs following pre-incubation storage

Zusammenfassung Veränderungen des Hühnereiweisses durch Lagerung können in der Immunpräzipitation (Ouchterlony-Test) erfasst werden.

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Scientific Paper No. 2915, College of Agriculture, Washington State University, Project 1255. This investigation was supported in part by Research Grant No. 5544 from Division of General Medical Sciences, U.S.P.H.S.     

The effect of an ethanol extract of catnip (Nepeta cataria) on the behavior of the young chick

Summary The alcohol extract of catnip has a biphasic effect on the behavior of young chicks. Low and moderate dose levels (25–1800 mg/kg) cause increasing numbers of chicks to sleep, while high dose levels (i.e. above 2 g/kg) cause a decreasing number of chicks to sleep.     

Lipids in the egg shell of the ostrich (Struthio camelus) (author's transl)]

The egg shell (with cuticle) of the ostrich contained a total lipid concentration of 0.19 mg/g egg shell; the phospholipids constituted the major portion, among the neutral' lipids cholesterol esters, cholesterol and free fatty acids were identified. The egg shell lipids showed a stimulating effect on the crystal growth of calcium carbonate; the highest rate of crystal growth was observed with the phospholipid fraction.     

Spontaneous potentials from explants of embryo chick pons (myelencephalon) in culture

Zusammenfassung Vorkommen und Form spontaner Potentialen aus Myencephalon-Explantaten (Nuclei-Region der Kranialnerven 5–7) 11 Tage alter Hühnchenembryonen in Gewebekultur werden beschrieben. Die Registrierung erfolgte während mehr als 120 h.

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This work was done under a U.S. Navy Contract, NONR 1598(04).     

Induction of alloxan diabetes in obese-hyperglycemic mice (genotype obob)

Zusammenfassung Nachweis, dass fettsüchtige, hyperglykämische Mäuse nach Alloxan-Injektion einen erhöhten Blutzuckerspiegel und herabgesetzte Insulinkonzentrationen im Vergleich mit den unbehandelten Kontrolltieren zeigen. B-Zellen der Langerhansschen Inseln zeigen nach Alloxan nekrotische Veränderungen.

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This work was supported by the Swedish Medical Research Council, the Swedish Diabetes Association and the Nordic Insulin Foundation.     

Induction of deoxyribonucleic acid degradation in Escherichia coli by ozone.

Cell survival and deoxyribonucleic acid (DNA) degradation wave measured for wild-type Escherichia coli B251 cells after exposure to different concentrations of ozone. The results show that extensive breakdown of DNA occurs after ozonation and that the extent of ozone-induced DNA degradation generally correlates with the colony-forming ability of the cells.     

The effect of an ethanol extract of catnip (Nepeta cataria) on the behavior of the young chick.

The alcohol extract of catnip has a biphasic effect on the behavior of young chicks. Low and moderate dose levels (25--1800 mg/kg) cause increasing numbers of chicks to sleep, while high dose levels (i.e. above 2 g/kg) cause a decreasing number of chicks to sleep.     

Increase in muscle fibres in the lateralis muscle (white portion) of Mugilidae (Pisces,Teleostei)

Summary A cycle of postlarval growth is described in the white portion of the lateralis muscle of Mugilidae. The cycle was identified histochemically by the myosin-ATPase reaction.This work was supported by a CNR grant (Oceanografia e fondi marini project).     

Exotoxin in malaria infection (Plasmodium berghei)

Zusammenfassung Swiss Mäuse, die unbehandelt eine Infektion mitPl. berghei niemals überleben, wurden mit Serum von frisch infizierten Tieren (12. Infektionstag) immunisiert und anschliessend infiziert. Obgleich sich danach in allen Fällen eine schwere Parasitämie entwickelte, heilten 44% derjenigen Tiere nach dem 35. Infektionstag spontan aus, die mit 3 mg Serumprotein/Maus immunisiert waren. Wegen der offensichtlich eingetretenen Immunoblockade überlebten dagegen nach Immunisation mit höheren Proteinmengen (mehr als 30 mg per Maus) keine Tiere.     

Zn(II)-activated acid phosphatase in liver and metanephros of developing chick

Résumé Pendant le développement embryonnaire du poulet, une phosphatase acide activée au Zinc(II) est présente dans le foie et le métanéphros, mais non dans le cerveau et le cur. L'élévation maximum du taux de cet enzyme se produit dans le foie et dans le métanéphros à des stades différents. Son développement semble être en rapport avec le développement fonctionnel des organes concernés.     

Induction of a 70,000 dalton protein in hypertrophic rat heart

Two-dimensional gel electrophoresis analysis of the product of in vitro translation of polyadenylated RNAs extracted from rat heart rendered hypertrophic by aortic constriction, shows a new protein species not present in the map of control hearts. The same is also obtained when hypertrophy is induced by treatment with thyroxine.     

Induction of a 70,000 dalton protein in hypertrophic rat heart

Summary Two-dimensional gel electrophoresis analysis of the product of in vitro translation of polyadenylated RNAs extracted from rat heart rendered hypertrophic by aortic constriction, shows a new protein species not present in the map of control hearts. The same is also obtained when hypertrophy is induced by treatment with thyroxine.     

Dose relationships of morning bright white light in seasonal affective disorders (SAD)

Summary Bright white full spectrum light (>2500 lux) can improve depressive symptomatology in a selected group of patients with recurrent autumn and winter depression. This crossover study demonstrates that 0.5-h morning white light is not an effective treatment, whereas 2-h is.