Cellular localization of androgen in the brain and pituitary after the injection of tritiated testosterone

Zusammenfassung Nach Injektion von³H-Testosteron wurde mit Hilfe der Trocken-Autoradiographie eine selektive Aufnahme und Speicherung von Radioaktivität in Kernen spezifischer Neurone im Hypothalamus, in der präoptisch-parolfaktorischen Region, im Septum, im Hippokampus und in der Amygdala beobachtet. Im Hypophysenvorderlappen war Radioaktivität in den Zellkernen von Basophilen nachweisbar. Die autoradiographischen Ergebnisse unterstützen das Konzept eines doppelten Androgen-Feedbacks auf der Ebene des Zentralnervensystems und der Hypophyse.

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We thank Mrs.Gerda Michalsky and Mrs.Anu Turnbull for their technical assistance. This study was supported by PHS grant No. AM1 929 to W.E.S. and a grant from the Rockefeller Foundation to the Laboratories for Reproductive Biology, Chapel Hill, North Carolina.     

Autoradiographic localization of radioactivity in female rat neocortex after injection of tritiated estradiol

Zusammenfassung Durch autoradiographische Untersuchung wurde die Einlagerung von Radioaktivität in ein begrenztes Gebiet des Neocortex der Rattenweibchen nach einmaliger Verabreichung von³H-Östradiol im Alter von 5 bis 50 Tagen nachgewiesen. Die reduzierten Silberkörner zeigen eine vorwiegend perinukleare Akkumulation, was für die Zellen der östrogen-Zielgewebe typisch ist.     

Cellular and subcellular localization of3H-diethylstilbestrol in the pituitary,oviduct and uterus of the female rat

Summary Female rats were injected with³H-diethylstilbestrol. The pituitaries, oviducts, and uteri were removed and processed for autoradiography. The nuclear uptake and retention in these tissues appeared similar to that seen after the injection of³H-estradiol except in the pars nervosa of the pituitary gland.This study was supported by US PHS Career Development Award No. NS00164 and PHS Grant No. NS21933.     

Cellular and subcellular localization of3H-estradiol or its metabolites in the pituitary of the neonatal female rat

Zusammenfassung Bei 2 Tage alten Ratten wurde markiertes Oestradiol-17 injiziert. Im Hypophysenvorderlappen waren weniger als 10% der Zellkerne markiert. Diese Zellen befanden sich vorwiegend in der Peripherie. Im Zwischen- und Hinterlappen fanden sich keine markierten Zellen. Eine Vorbehandlung mit nicht markiertem Oestradiol hemmte die nukleare Aufnahme der Radioaktivität, was beweist, dass diese mit Oestradiol oder dessen Metaboliten verbunden sein muss.     

Effects of testosterone propionate on LH and testosterone concentrations before and after the injection of gonadoliberin in young bulls pre-treated with dexamethasone]

This preliminary study has been performed on four young twin bulls, one pair aged 6 months and the other one aged 12 months. Plasma LH and testosterone were assayed from frequent blood sampling. Testosterone propionate (TP) following dexamethasone treatment did not significantly influence the LH response to gonadoliberin compared to the twin controls, although testosterone concentrations were high in those TP treated animals.     

Immunofluorescent localization of β-Corticotropin in the rat pituitary

Zusammenfassung Unter Verwendung eines spezifischen Anti-^1–24-Corticotropin-Serums vom Kaninchen wurde -Corticotropin immunhistologisch in der Rattenhypophyse nachgewiesen. Neben den Epithelien der Pars intermedia konnte kortikotropes Hormon in einem besonderen, mukoiden Zelltypus der Adenohypophyse lokalisiert werden.     

Age-dependent response of lactate dehydrogenase of pituitary of rat to testosterone

Summary The effects of time and various doses of testosterone on the responsiveness of lactate dehydrogenase of pituitary of 7-, 38- and 78-week-old rats were studied. The activity of the enzyme increases in 78-week-old rats. Castration decreases the enzyme activity at all ages. Maximum increase in the enzyme activity is seen with 50 and 100 g of testosterone 4 h after administration of hormone to castrated rats. No further time and dose-dependent effect is observed. The magnitude of increase for the enzyme is higher at the age of 38 weeks and decreases in 78-week-old rats.To whom all correspondence should be addressed. The authors are grateful to Professor M.S. Kanungo, Biochemistry Laboratory, Department of Zoology, Banaras Hindu University for the suggestions and encouragement. K.V.G. thanks the PL-480 for a research fellowship.     

Labelling of ribosomal RNA peaks in the liver of rats after administration of tritiated fluorouracil

Zusammenfassung Nach i.p. Injektion von 5-Fluorouracil-³H wurde bei Ratten zu verschiedenen Zeiten die RNS-Neubildung der Leber bestimmt. Die Resultate sprechen dafür, dass Fluorouracil in die ribosomale RNA der Leber eingebaut wird.

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This work was supported by grants from the Swedish Medical Research Council (Project No. K68-12X-623-04), the Swedish Cancer Society (Project No. 95-K68-02X), the Faculty of Medicine, University of Uppsala, Sweden, and the Swedish Society for Medical Research.     

Differences in in vitro incorporation of tritiated deoxycytidine and tritiated thymidine into human lymphocytes

Summary Human lymphocytes stimulated in vitro by PHA or PWM incorporate³H-CdR into DNA, but less well than³H-TdR. More³H-CdR is incorporated in populations enriched for T cells. No³H-CdR is used by cells stimulated by lipopolysaccharides under circumstances in which³H-TdR is incorporated. We conclude that human T cells and B cells differ in their ability to use³H-CdR.     

Differences in in vitro incorporation of tritiated deoxycytidine and tritiated thymidine into human lymphocytes.

Human lymphocytes stimulated in vitro by PHA or PWM incorporate 3H-CdR into DNA, but less well than 3H-TdR. More 3H-CdR is incorporated in populations enriched for T cells. No 3H-CdR is used by cells stimulated by lipopolysaccharides under circumstances in which 3H-TdR is incorporated. We conclude that human T cells and B cells differ in their ability to use 3H-CdR.     

Differences in utilization of tritiated thymidine and tritiated deoxycytidine by rat lymph node cells

Summary Large lymphoid cells and plasma cells in antigenically stimulated rat lymph nodes retain less label after injection of³H-CdR than after injection of³H-TdR. There is no difference amongst small lymphocytes. The data are consistent with a defect in the utilization of³H-CdR in the late stages of B cell maturation in the rat.