Antibodies against connective tissue in collagen diseases

Zusammenfassung In SerenFebris rheumatica-Kranker wurden Autoantikörper gegen isolierte Hyaluronsäure durch Kollodion-Agglutination gefunden.

---

---

Statistical evaluation:V. Malý. Technical assistence:Alena Sovákova.     

Antibodies of aborted bovine fetuses respond to placental structures

Summary IgG isolated from aborted bovine fetuses was found to be directed not only to a diaplacental infectious agent but, in half of the cases, also to the site of infection, i.e. the placental tissue. The techniques used were immunohistochemistry and agarose immunoelectrophoresis. These findings suggest a hitherto unrecognized pathological mechanism of abortion.     

Antibodies of aborted bovine fetuses respond to placental structures

IgG isolated from aborted bovine fetuses was found to be directed not only to a diaplacental infectious agent but, in half of the cases, also to the site of infection, i.e. the placental tissue. The techniques used were immunohistochemistry and agarose immunoelectrophoresis. These findings suggest a hitherto unrecognized pathological mechanism of abortion.     

Guinea-pig adipose tissue responsiveness to catecholamines

Zusammenfassung Es wurde festgestellt, dass Meerschweinchen-Fettgewebe auf Catecholamine weniger reagierte als Rattengewebe. War die Inkubation zerkleinerten Gewebes mit Aminen langfristig, so zeigte das Meerschweinchen-Fettgewebe eine lipolytische Aktivität.     

Defining the resistance to oxygen transfer in tissue hypoxia

Studies of O2 supply in freshly isolated adult mammalian cells provide new insight into the factors that limit mitochondrial oxygenation in vivo. Of particular importance, mitochondria are present at high densities and often in apparent clusters, both of which contribute to local O2 gradients under hypoxic conditions. Current evidence indicates that the mitochondrial distribution is a component of the differentiated phenotype of adult mammalian cells and that specific motors and anchoring mechanisms are present to allow redistribution in response to developmental, physiological and pathological challenges. To compare the importance of resistance to O2 transfer under different conditions and at different sites along the supply path in vivo, a simple mathematical expression of relative resistance to O2 supply is introduced. Under various pathophysiological conditions, this resistance increases in specific regions of the pulmonary, circulatory or cellular supply path and results in O2 deficiency in the mitochondria. Regardless of cause, the relative resistance increases dramatically in the vicinity of mitochondrial clusters during hypoxia.     

Response of neuronal lysosomes to anoxia in tissue culture of mammalian cerebellum

Zusammenfassung In Gewebekulturen wurde die zytochemische Veränderung der Lysosomen durch Sauerstoffentzug an Säugetierkleinhirnnervenzellen mit der Gomori-Säurephosphatasemethode studiert.     

Hemodynamic studies in acute venous stasis edema in rats

An increase in venous pressure in the rat tail is known to result in acute edema. Acute venous stasis edema of the rat tail was induced by applying a force-controlled banding of standard tension (200 g) proximally for a period of 6-12 h. The hemodynamic changes of acute venous stasis edema were evaluated using non-invasive plethysmography, fluorescence angiography, computer thermography and invasive radioactive microsphere techniques. It is shown here that reduction of tail circulation to 40% of the control value is followed by prolonged vascular disorder characterized by genesis of reversible edema, increased total blood flow to the tail and decreased local cutaneous blood flow, without affecting the general hemodynamics. The cutaneous circulation (decreased blood flow) seems to be principally involved in the edemogenic response, whereas the deeper vessels (hyperemia) may or may not play a determinant role in acute experimental venous stasis edema in rats.     

Hemodynamic studies in acute venous stasis edema in rats

Summary An increase in venous pressure in the rat tail is known to result in acute edema. Acute venous stasis edema of the rat tail was induced by applying a force-controlled banding of standard tension (200 g) proximally for a period of 6–12 h. The hemodynamic changes of acute venous stasis edema were evaluated using non-invasive plethysmography, fluorescence angiography, computer thermography and invasive radioactive microsphere techniques. It is shown here that reduction of tail circulation to 40% of the control value is followed by prolonged vascular disorder characterized by genesis of reversible edema, increased total blood flow to the tail and decreased local cutaneous blood flow, without affecting the general hemodynamics. The cutaneous circulation (decreased blood flow) seems to be principally involved in the edemogenic response, whereas the deeper vessels (hyperemia) may or may not play a determinant role in acute experimental venous stasis edema in rats.Acknowledgment. The authors thank Mr G. Ponard for his technical assistance.     

Fenestrated capillaries in ‘venous patch’ adventitia

Summary Fenestrated capillaries in venous patch adventitia are seen during the 2nd week after microsurgery. The fenestrae contain diaphragms. These capillaries are possibly transitory structures which appear during injury cicatrization. The importance of these capillaries during transcappillary exchange in injuried tissue is discussed.The technical assistance of M. Guerricabeitia is gratefully acknowledged.     

Fluorescent studies of antibodies to rabbit male urogenital tissue

Zusammenfassung Immunofluoreszenzoptische Vorversuche mit Seren männlicher Kaninchen, deren KoagulationsdrÜsen Schädigung durch wiederholtes Tieffrieren in situ ausgesetzt waren, lassen vermuten, dass diese Sera Antikörper enthalten, die mit dem Zytoplasma von Azinusepithelzellen und anderen Anteilen der KoagulationsdrÜsen reagieren.     

The response of human skeletal muscle tissue to hypoxia

Hypoxia refers to environmental or clinical settings that potentially threaten tissue oxygen homeostasis. One unique aspect of skeletal muscle is that, in addition to hypoxia, oxygen balance in this tissue may be further compromised when exercise is superimposed on hypoxia. This review focuses on the cellular and molecular responses of human skeletal muscle to acute and chronic hypoxia, with emphasis on physical exercise and training. Based on published work, it is suggested that hypoxia does not appear to promote angiogenesis or to greatly alter oxidative enzymes in skeletal muscle at rest. Although the HIF-1 pathway in skeletal muscle is still poorly documented, emerging evidence suggests that muscle HIF-1 signaling is only activated to a minor degree by hypoxia. On the other hand, combining hypoxia with exercise appears to improve some aspects of muscle O₂ transport and/or metabolism.     

Antibodies in the course of resorption of the ehrlich cancer heterotransplant in rats

Résumé Chez les rats jeunes auxquels on a inoculé le cancer ascitique d'Ehrlich 1–2 jours après la naissance, la tumeur atteint une grande dimension et disparaît au bout de 14–31 jours. Chez des rats de 14 jours, le sérum a une action cytotoxique sur les cellules du cancer d'Ehrlich et agglutine celles-ci à partir du 21^e jour.