Chromatographic separation of phospholipase a from a histamine releasing component of brazilian rattlesnake venom (Crotalus durissus terrificus)

Zusammenfassung Die chromatographische Auftrennung von Klapperschlangen- (Crotalus durissus terrificus) Gift ermöglicht, den Unterschied zwischen Phospholipase A und einer Histamin freisetzenden Komponente dieses Giftes zu bestimmen. Letztere ist ein hitzelabiles, nicht dialysierbares Protein (enzymatisch), leicht von Crotamin unterscheidbar. Phospholipase A ist nicht fähig, Histamin aus isolierten Ratten-Mastzellen freizusetzen. Der hier isolierte Histaminliberator wirkt nicht auf mit 2,4-Dinitrophenol vorbehandelte Mastzellen.

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This work was aided by Grant No. 49-092-66-G101, US Army Research Office.     

Isolation and characterization of a cystine-containing octapeptide from silk

Zusammenfassung Isolierung und Strukturaufklärung eines Oktapeptides aus Seidenfibroin ausBombyx mori.     

Acylfulvenes,a new class of potent antitumor agents

Acylfulvene, derived from the sesquiterpene illudin S by treatment with acid (reverse Prins reaction), is far less reactive to thiols than illudin S. However, it is reduced readily to an aromatic product, in the same way as illudin S. This may explain its greatly improved therapeutic index compared to that of the parent compound.Unfortunately, publication was held up due to postal delay.     

The structure of a novel antitumor antibiotic,saframycin A

Summary A structure is assigned to saframycin A, a novel antitumor antibiotic fromStreptomyces lavendulae No. 314, on the basis of¹³C NMR-spectral data.     

Chromatographic evidence for murein from the bacteroid symbiotes ofPeriplaneta americana (L)

Zusammenfassung Papierchromatographisch wurde Murein in symbiontischen Bakteroiden derPeriplaneta americana nachgewiesen. Die Murein-Komponente Glukosamin und der Murein-Anzeiger Muramic-Säure wurden mittels Bakteroid-Hydrolysaten identifiziert. Das Vorhandensein von --Diaminopimelic-Säure jedoch konnte nicht einwandfrei festgestellt werden.

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This is paper No. 6175, Scientific Journal Series, Minnesota Agricultural Experiment Station, St. Paul (Minnesota, USA). The work was supported by U.S. Public Health Service Research Grant No. AI 00961, from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health.     

Isolation and characterization of a new hemagglutinin from the red algaGracilaria bursa-pastoris

Summary A new agglutinin has been isolated from the red algaGracilaria bursa-pastoris by affinity chromatography on a yeast mannan-Cellulofine column. This agglutinin was isolated as a monomeric glycoprotein with a relatively low molecular weight. It had an isoelectric point of 4.7 and contained large amounts of Gly, Asx and Glx. It agglutinated trypsin-treated rabbit erythrocytes at the low concentration of 30 ng/ml. The activity was inhibited only by glycoproteins bearing N-glycans. This agglutinin also showed mitogenic activity for mouse splenic lymphocytes.     

Isolation and characterization of a high-molecular-weight glycoprotein from the endometrium of porcine uteri

A novel glycoprotein was isolated from the endometrium of porcine uteri. This high-molecular-weight glycoprotein consisted of 25% of protein and 73% of carbohydrate. The carbohydrate composition was quite characteristic in that equimolar N-acetylglucosamine and galactose were major constituents. Its unique nature makes it distinguishable from hitherto-reported glycoproteins.     

Isolation and characterization of a high-molecular-weight glycoprotein from the endometrium of porcine uteri

Summary A novel glycoprotein was isolated from the endometrium of porcine uteri. This high-molecular-weight glycoprotein consisted of 25% of protein and 73% of carbohydrate. The carbohydrate composition was quite characteristic in that equimolar N-acetylglucosamine and galactose were major constituents. Its unique nature makes it distinguishable from hitherto-reported glycoproteins.     

Transmembrane movements of lipids

Membranes allow the rapid passage of unchanged lipids. Phospholipids on the other hand diffuse very slowly from one monolayer to another with a half-time of several hours. This slow spontaneous movement in a pure lipid bilayer can be selectively modulated in biological membranes by intrinsic proteins. In microsomes, and probably in bacterial membranes, non-specific phospholipid flippases allow the rapid redistribution of newly synthesized phospholipids. In eukaryotic plasma membranes, aminophospholipid translocase selectively pumps phosphatidylserine (PS) and phosphatidylethanolamine (PE) from the outer to the inner leaflet and establishes a permanent lipid asymmetry. The discovery of an aminophospholipid translocase in chromaffin granules proves that eukaryotic organelles may also contain lipid translocators.     

Isolation and partial characterization of a phytotoxic glycoprotein from culture filtrates ofRhynchosporium secalis

Summary Rhynchosporium secalis (Oud.) Davis produces a phytotoxic compound with a mol. wt of 275×10³ which is able to induce chlorotic symptoms in both susceptible and resistant barley leaves. Collectively, the data suggest that the toxin is a glycoprotein. Mild base treatment, by elimination, indicates that threonine and serine are involved in o-glycosidic linkages with the carbohydrate moiety. Sugar residues occur in the molecule in the ratio of mannose, rhammnose, galactose, glucosamine 13.6111.Acknowledgments. We acknowledge the assistance of Mr M. McNeil and P. Albersheim in doing the sugar analyses shown in this report. Financial support for this project was provided by a BARD grant 1-31-79, the French government, and the Montana Ag. Expt. Station.     

Isolation and characterization of a new hemagglutinin from the red alga Gracilaria bursa-pastoris

A new agglutinin has been isolated from the red alga Gracilaria bursa-pastoris by affinity chromatography on a yeast mannan-Cellulofine column. This agglutinin was isolated as a monomeric glycoprotein with a relatively low molecular weight. It had an isoelectric point of 4.7 and contained large amounts of Gly, Asx and Glx. It agglutinated trypsin-treated rabbit erythrocytes at the low concentration of 30 ng/ml. The activity was inhibited only by glycoproteins bearing N-glycans. This agglutinin also showed mitogenic activity for mouse splenic lymphocytes.