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1.
选取经资源调查及品质分析后获得的优良植株的顶芽和腋芽为材料,诱导腋芽的萌发和不定芽增殖生根.结果表明:从3月下旬到5月上旬是菠萝蜜组织培养大田取材的最佳时间,该时期接种材料污染率(〈50%)及褐变率(〈5%)均较低;启动培养的培养基最佳组合是MS+6-BA2.0mg/L+KT1.0mg/L+GA。0.5mg/L+蔗糖20g/L,腋芽诱导率为44.6%,KT含量较高(1.0mg/L)有利于腋芽的诱导,诱导率平均为(40%);不定芽增殖培养的培养基最佳组合是MS+6-BA1.5mg/L+KT0.1mg/L+GA。0.5mg/L+蔗糖40g/L,增殖率为2.61,蔗糖含量较高(40g/L)有利于不定芽的增殖,增殖率平均为2.34;生根培养以1/2MS+IBA1.5mg/L+NAA0.2mg/L最好,接种8d开始生根,生根率15.8%,15d根长3~4cm,平均根数3~4条.  相似文献   

2.
福建省山樱花的组织培养及植株再生   总被引:5,自引:0,他引:5  
以春季萌发的福建山樱花嫩枝为外植体诱导丛生芽 。在1/4MS+BA1.0mg/L IBA0.1mg/L的培养基上,其丛生芽诱导率达87.5%;在继代培养中选择MS+BA1.0mg/L IBA0.1mg/L GA30.3mg/L或KT1.0mg/L NAA0.1mg/L BA30.3mg/L培养基,不定芽增殖较快。赤霉素对其不定芽的伸长有明显效果,当芽伸长在3-4cm时,切下置于生根培养基1/2MS+NAA1.0mg/L IBA1.0mg/L BA0.75mg/L中,生根率达90%以上,移栽成活率达95%。  相似文献   

3.
以茉莉的下胚轴为外植体,研究了茉莉直接不定芽器官发生途径的再生体系.结果表明:以MS为基本培养基,附加0.5mg/L BAP和0.01mg/L NAA的诱导效果最佳,其不定芽再生率高达92%,外植体平均不定芽数目为4.2.适宜不定芽增殖的最佳培养体系为MS+1.0mg/L BAP+0.1mg/L NAA.1/2MS+0.5mg/LIBA+0.5mg/L IAA+100mg/L适于再生幼茎的生根,生根率达91%,生根后经移栽成活.  相似文献   

4.
以猪笼草的幼嫩茎段为外植体进行离体组织培养及植株再生,研究结果表明:以液体培养基1/2MS+6-BA1.0mg/I.+NAA0.1mg/L对芽的初始诱导以及固体培养基1/2MS+6-BA2.0mg/L+NAA0.1mg/L对不定芽的增殖效果最好,增殖率达419%;生根培养基以1/4MS+IBA0.5mg/L+活性碳(0.01%)为最佳,生根率可达100%.  相似文献   

5.
探索了米邦塔食用仙人掌组培快繁技术。得出不定芽诱导使用培养基MS+6-BA3.0mg/L+NAA0.1mg/L,外植体分化率达77.3%;继代增殖使用培养基MS+6-BA1mg/L+KT0.5mg/L+NAA0.3mg/L,试管苗平均增殖倍数达10.19,结合反复利用原外植体的方法,增殖效率可进一步提高;生根培养基使用1/2 MS+NAA0.1mg/L+IBA0.1mg/L,生根率达100%,且根系发育优良;驯化及试管苗入地后,以保湿为主要管理措施,可保证移栽成活率在95%左右。  相似文献   

6.
选用741杨的茎段和生根组培苗叶片为外植体,采用不同浓度组合的BA和NAA对741杨的离体快繁和叶片再生体系进行了研究。结果表明:741杨芽增值的最佳培养基为MS+6-BA 0.5mg/L+NAA 0.1mg/L;最佳生根培养基为MS+IBA 0.3mg/L;741杨叶片不定芽诱导的最佳培养基为MS+6-BA 1.0mg/L+NAA 0.1mg/L。试验还研究了不同着生位置的叶片以及叶片在培养基上的放置方式对741杨叶片再生的影响。  相似文献   

7.
采用DN113杨休眠枝水培芽为外植体,进行组培育苗试验,结果表明:不同光照下萌发的芽体对不定茅的诱导率有影响,完全闭光条件下萌发的黄化芽诱导不定芽的诱导率最高;筛选出DN113杨组培育苗的适宜诱导培养基为MS+BA1.0+NAA0.1、适宜继代培养基为MS+BA1.5+KT0.5+NAA0.15、适宜生根培养基为1/2MS+IBA0.3+NAA0.2。  相似文献   

8.
蒜头果组织培养再生系统的初步研究   总被引:7,自引:0,他引:7  
以蒜头果(Malania oleifera)种子萌发获得的无菌苗进行离体培养的初步研究.结果表明:MS+6-BA1.5mg/L+NAA0.1mg/L激素组合能够较好地诱导芽的初始分化和增殖;适宜的继代增殖培养基为MS+6-BA1.0mg/L+NAA0.2mg/L;采用1/2MS+IBA0.5mg/L为生根培养基,生根率为45.0%;移栽到河沙的生根苗成活率为52.5%.  相似文献   

9.
以杜仲(EncommiaulmoidesOliver)当年生枝条的叶片、带芽茎段为外植体,基本培养基为MS,附加不同浓度的BA、KT和NAA.最佳芽诱导培养基为:MS KT1 0mg/L NAA0 1~0 5mg/L;MS BA2 0mg/L NAA0 1~0 5mg/L,最佳芽丛增殖培养基为:MS KT2 0mg/L NAA0 1~0 2mg/L;MS BA1 0~2 0mg/L NAA0 1~0 2mg/L;MS KT2 0mg/L IBA0 1~0 2mg/L;MS BA1 0~2 0mg/L IBA0 1~0 2mg/L,单芽增殖培养基:MS KT1 0mg/L NAA0 1mg/L,生根培养基:13MS KT0 1mg/L NAA0 5mg/L.  相似文献   

10.
新铁炮百合组织培养和快速繁殖研究   总被引:39,自引:4,他引:35  
以日本新铁炮百合鳞茎及叶为外植体成功获得再生植株,并建立了快速无性繁殖系。鳞片和叶的最佳诱导培养基分别为MS+0.4-1mg/L BA 0.2mg/L NAA;MS 0.1mg/L(或0.5mg/L)BA+0.1mg/L NAA或0.5mg/L IBA。芽增殖培养基选MS+0.2-0.5mg/L BA 0.1mg/L NAA或0.2mg/L IBA。生根培养基为1/2MS+0.1mg/L IBA 0.1%活性炭。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

13.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

14.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

15.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

16.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

17.
For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the  相似文献   

18.
Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of  相似文献   

19.
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with  相似文献   

20.
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