新疆普氏野马鼻肺炎病毒的分离鉴定

从新疆昌吉州吉木萨尔野马饲养繁殖中心送检的野马病料中分离到一株病毒,并对其进行了全面鉴定．该病毒株在ＢＨＫ－２１细胞上连传６代出现典型细胞病变,用适应ＢＨＫ－２１细胞的病毒接种鸡胚成纤维细胞、乳豚鼠肾原代细胞、豚鼠睾丸细胞均出现程度不同的细胞病变．在电镜下可见到典型的马鼻肺炎病毒粒子（疱疹病毒样颗粒）．病毒对５－碘脱氧尿核苷、氯仿、乙醚等敏感．在ｐＨ３下失活,５６℃３０ｍｉｎ灭活．病毒能被马鼻肺炎病毒标准阳性血清所中和．病毒接种乳豚鼠出现典型致死性肝炎．结果表明所分离病毒为马鼻肺炎病毒,并将该毒株定名为ＰＨ９３－１株     

Ⅳ型胶原放射免疫分析方法的建立及临床应用

目的：应用单克隆技术制备鼠抗人Ⅳ型胶原（Ⅳ－C）单克隆抗体。用氯胺－T方法标记Ⅳ－C，应用免疫竞争法建立Ⅳ－c放射免疫分析方法，其检测范围20－500ug／L。方法和结果：通过特异性、精密度、准确性、亲和常数等实验方法学检验，表明Ⅳ－C放射免疫分析方法符合临床应用要求。应用该方法检测30例肝炎患者、15例肝硬化患者、35例NIDDM患者25例甲亢患者；除NDDM、甲亢外，其余患者血清浓度明显高于正常人（P＜0．01）；NIDDM患者，甲亢患者Ⅳ－C血清浓度高于正常人（P＜0．05）。结论：Ⅳ－C血清浓度检测在肝病、肾病及相关疾病的研究有一定的临床价值。     

抗鱼卵透明带单克隆抗体淋巴细胞杂交瘤的建立及初步鉴定

详细介绍了抗鱼（花鲢Anstichthysnobilis）卵透明带单克隆抗体淋巴细胞杂交瘤的制备及鉴定方法，用佛氏佐剂乳化热溶花鲢鱼卵透明带，然后免疫雌性BALB／C小鼠，取其脾细胞与骨髓瘤Sp2／0融合产生杂交瘤，经3次再克隆后，用间接ELISA方法筛选出一株抗鱼卵透明带的单克隆抗体杂交瘤，经免疫双扩散试验测得该种单克隆抗体为IgG1，经过染色体检查知其染色体数目在86～108之间，为93.6±3.81，杂交瘤细胞染色体经检查发现有中间和亚中着丝点染色体，符合杂交细胞染色体特征。     

山茶属五种野生植物种子萌发试验分析

历时三年，对贵州特有的美丽红山茶（CamelliadelicataY．K．Li）、长柱红山茶（C．longistyllaYKLietChang）、小黄花茶（C．luteofloraYKLi）、狭叶瘤果茶（CnerifoliaChang）和野生油茶（ColeiferaAbel.）（广布种）等自然野生种类的结实与繁殖进行了研究．实验结果为：1，成花自然结实百分率分别为40．3，30．0，22．5，20．8，45．6：成熟种子百分率为结实率的60．0，50．3，60．0，58．5，60．2；种子带病虫百分率为结实率的30．0，38．0，28．0，16．0，28．6：在原自然分布地林区设15m×15m样方统计种子自然萌发百分率，其平均数分别为3．0，2．5，3．0，2．5，8．6：而苗床播种出苗率则为86．2，90．076．0，76．1，85．4；2，受精不发育或不完全发育现象普遍存在，调查结果得发育完全的种子百分率为50．0，60．6，30．5，30．0，46．5：3，五种植物的成熟种子在5～22℃，相对湿度≥60％的暗光或无光条件下30天均开始萌芽，最适萌发气温为17±1℃，最适前发湿度为70±5％，但同时播入土中的种子，极少数可延迟1～2年萌发．种子的形成和萌发是授粉、受精、发育生理与其环境因子综合作用的结果，采用科学方法促进种胚发育，提高种子成熟率和种子活力，解决种子出苗整齐，培育壮苗等关键技术，在     

具有纳米质点性质的MFI型硅沸石微细晶粒的合成及表征(英)

分别以白炭黑（Ⅰ）和硅溶胶（Ⅱ）为硅源，在（TPA）2O－Na2O－SiO2－H2O体系（TPA为四丙基铵）中，反应温度为60℃，合成了高结晶度纯硅沸石．用扫描电子显微镜（SEM）法测得的晶粒大小分别为0．20μm（Ⅰ）和0．45μm（Ⅱ）；用粉末X射线衍射（XRD）线宽法测得的晶粒大小为0．02μm．与高温（180℃）合成的硅沸石样品相比较，低温合成的微细晶粒硅沸石样品已具备纳米级材料的若干特性．微细晶粒硅沸石的正己烷吸附量和比表面都非常大．XRD谱，红外吸收光谱（FT－IR），29Si魔角固体核磁共振（29SiMASNMR）和热重分析（TG／DTG／DTA）的研究证明，微细晶粒硅沸石的结构破坏温度和单斜／正交对称性相转变温度明显地比高温合成的硅沸石低，硅羟基缺陷也明显地增加．0．20～0．45μm的微细晶粒硅沸石的比表面几乎与胶体质点相同，它的外表面积达100m2／g，中孔容积为0．10～0．12ml／g，平均孔径为2．6～2．7nm．这表明微细晶粒硅沸石实际上是纳米质点的结合体．     

白血病小鼠模型中P53、C-myc基因表达的变化研究

探讨DNA、RNA病毒诱导小鼠发生白血病的机制。用单纯疱疹病毒Ⅱ型（HSV－2）和C型RNA病毒诱导70只昆明小鼠，光学显微镜检测HSV－2、C型病毒诱导发生白血病的成功率。正常对照组为30只昆明小鼠。免疫组化法检测HSV－2、C型病毒致瘤后P^53、G－myc基因表达的变化。70只昆明小鼠中有47只发生白血病，成功率为67．1％。47只白血病小鼠P^53、C－myc蛋白阳性表达率均比正常对照组高（P＜0．05）；23只未形成白血病的实验组小鼠P^53、C－myc蛋白阳笥表达率均与正常对照组无显差异（P＞0．05）。HSV－2、C型病毒可以通过调节P^53、C－myc基因的表达从而诱导小鼠白血病的发生。     

野木瓜皂甙对HL60肿瘤细胞增殖的影响

研究野木瓜皂甙（Stauntoniae Saponin，SS）提取物对人类白血病细胞系HL60增殖的影响，为中草药抗肿瘤提供实验依据．以不同浓度SS作用于HL60细胞，然后用MTT比色法测定它对HL602细胞增殖的影响．当浓度为18mg／L时，SS能明显地促进HL60细胞的增殖（P〈0．01）；浓度大于90mg／L时，SS对HL60细胞增殖产生抑制作用；当浓度为2253mg／L时，抑制率高达63．15％（P〈0．01）．浓度大于18mg／L后，细胞增殖的抑制率与SS浓度呈线性相关，r=0．8928（P〈0．001）；SS的LC50为1652mg／L．野木瓜皂甙提取物对HL60细胞的增殖有明显的双向调节作用，即在较小范围的低浓度区域内有促进HL60细胞增殖的作用，浓度增大后则改变为抑制作用；浓度越大，抑制作用越强．     

母婴配对血清中TT病毒的分子鉴定

为了解TT病毒（TTV）在我国母婴间传播的可能及分子病毒学依据，合成引物（引物1：5′－ACAGACAGAGGAGAAGGCAACATG－3′；引物2：5′－CTGGCATTTTACCATTTCCAAAGTT－3′；引物3；5′－GGCAACATGTTATGGATAGACTGG－3′），采用半套式聚合酶链反应（hemi-nested PCR）方法，检测了40对母、婴母对血清中的TTV DNA，并对TTV DNA均阳性的一对母婴配对血清中PCR产物进行测序和序列分析，结果发现：（1）在40例产妇血清中，有5例经半套式PCR检出了TTV DNA，检出率为12.5%；40例脐血中，检出TTV DNA阳性血清1例，阳性率为2.5%，脐血呈阳性的新生儿母亲静脉血中TTV DNA也为阳性。（2）母婴同时检出TTV DNA的血清PCR产物经测序后进行序列比对，二者病毒核苷酸序列的同源性为1005；与日本G2b型代表株核苷酸同源性为98.6%，属于G2b亚型，研究结果表明：TTV存在母婴传播途径，TTV可经胎盘垂直传播。     

三角褐指藻（Phaeodactylum tricornutum）形态及其变异现象的研究

分别对青岛、浙江、上海和厦门四地的三角褐指藻藻种类及单个细胞克隆后多个藻株的形态及其变异现象进行了比较研究。青岛、上海藻种短期培养后，培养液细胞基本由梭形细胞组成；浙江和厦门藻种则基本由三出放射形细胞组成。正常培养液中卵形细胞比例均在0．1％以下。特定条件下，梭形和三出放射形细胞均能产生卵形细胞，卵形细胞也能发育成梭形或三出放射形细胞；三出放射形细胞可能经“突变”或“定向变异”转变成梭形细胞，而梭形细胞培养液中没有出现三出放射形细胞。培养液比重、温度及光照条件的改变短期内对细胞形态类型的转变没有明显影响；淡水和低温（4℃－8℃）的长期胁迫能使梭形和三出放射形藻株发生高比例的卵形细胞。     

论鸡传染性法氏囊病病毒细胞苗的制造和应用

目前使用的多种疫苗仍未能有效控制鸡传染性法氏囊病（ＩＢＤ）的发生，原因是疫苗质量难以保证和免疫程序或方法不发。解决的办法：病毒培养宜选用无特定病原（ＳＰＦ）鸡胚或非免疫健康鸡群的鸡胚，消化时间控制在１５ｍｉｎ左右，细胞数在１００－１５０万／ｍＬ，接毒量为０．１％－０．２％，小牛血清使用浓度为３－５％；疫苗冻干过程应在不断搅拌病毒培养液的同时控制加入保护剂的速度。免疫程序：无母源抗体或母源抗体不清雏     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with