共查询到20条相似文献,搜索用时 46 毫秒
1.
C Do?nel J P Blanchet 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1978,286(1):153-156
I and i blood group antigens have been looked for an Chick erythrocytes using purified and 125 I anti-I, anti-i and anti-I i antibodies. No I antigen is found but i determinants are detected on embryo and adult Chick erythrocytes. This i reactivity is different from the embryonic and adult antigens respectively specific for embryo and adult Chick erythrocytes. Treatment of the erythrocytes with nuraminidase increases the i reactivity. Incubation with papain reduces the fixation of anti-i antibodies suggesting that the i determinant of Chick erythrocyte could be linked to glycopeptide chains. 相似文献
2.
M Aupoix J Huppert P Vigier 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1976,282(14):1379-1382
Rous sarcoma virus produced by Chick embryo fibroblasts is inactivated by an antiserum prepared against uninfected fibroblasts in the presence, but not in the absence, of complement. This inactivation which demonstrates the presence of one or more antigens of the surface of the producer cell on the viral envelope, is due to virolysis. This is demonstrated by the release of the viral internal proteins and by the fact that the viral RNA becomes entirely degradable by RNase. 相似文献
3.
H. Stähelin 《Cellular and molecular life sciences : CMLS》1960,16(7):306-307
Summary Chick embryo fibroblasts, cultivatedin vitro, require higher concentrations of podophyllotoxin derivatives for complete mitotic arrest when the medium contains human adult or cord serum than when it contains horse, bovine, guinea pig, rat, mouse, or cock serum. This difference is due to a higher binding capacity of human serum for these compounds. No such difference was found with a colchicine and a peltatin derivative. 相似文献
4.
R Bassleer F De Paermentier J C Jamoulle C L Lapière 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1977,284(10):859-861
New thiopyrimidine derivatives have been synthesized. Among them, several inhibit the multiplication of Chick embryo cells cultivated in vitro: they provoke strong nucleolar alterations, prevent the cells from entering into mitosis and can give rise to cell polyploidisation as to DNA. 相似文献
5.
R Bassleer F de Paermentier A Lepoint M P Lhoest-Gauthier J C Jamoulle C L Lapière 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1978,287(4):369-371
A new thiopyrimidine derivative has been synthesized. It can inhibit cell multiplication in Chick embryo fibroblasts, in Mouse Ehrlich ascites tumor cells and in Rat hepatoma cells (line Rueber) cultivated in vitro. 相似文献
6.
B No?l-Courtey E Heinen 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1977,285(4):385-387
Chick embryo nerve cells from the lumbo-sacral spinal cord have been isolated by trypsinisation and cultivated in Rose chambers on polylysine-L as a substrate. The cells are analysed by scanning electron microscopy. The mode of adherence of these cells to the substrate and the modifications of the surface of the neuroblasts during their transformation into neurocytes are studied. 相似文献
7.
R Van De Walle-DuckertsBassleer F De Paermentier 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1977,284(8):651-653
Chick embryo fibroblasts cultivated in vitro are treated with trimethoprim. The mitotic activity is strongly depressed. DNA and overall RNA and protein syntheses are inhibited. However, these effects are observed only when the concentration of the drug in the culture medium is relatively high. 相似文献
8.
B Bernard M Aubery R Bourrillon 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1979,288(1):163-166
The variations of proteins and glycoproteins of Chick embryo fibroblasts are studied during development. This investigation is carried out using polyacrylamide disc gel electrophoresis in SDS. Two glycoproteins of high apparent molecular weight (250,000 and 200,000) undergo quantitative modification: they increase from the 8th to 12th day of development and then remain unchanged to the 16th day. They are cell surface components as suggested by fluorescamine labelling and trypsin sensitivity. The results are discussed in terms of relationship between tumor- and embryo cells. 相似文献
9.
Chick embryo liver primary cultures, when added with progesterone, exhibit, in comparison with the controls, a normal growth, a decline of both 3H-uridine uptake and incorporation into total RNA, a decline of 3H-leucine and 14C serine incorporation into the proteins. Progesterone is not able to stimulate phosvitin synthesis induction. 相似文献
10.
Chick embryo mesoderm cells were explanted to culture systems in vivo and in vitro and their subsequent movements were correlated with the external morphology as studied by SEM. In vitro cell movements are exaggerations of normal in vivo movements where a 2-dimensional substrate is encountered rather than a 3-dimensional environment. 相似文献
11.
P. Carinci A. Caruso R. Evangelisti E. Becchetti C. Calastrini 《Cellular and molecular life sciences : CMLS》1976,32(1):42-43
Summary Chick embryo liver primary cultures, when added with progesterone, exhibit, in comparison with the controls, a normal growth, a decline of both3H-uridine uptake and incorporation into total RNA, a decline of3H-leucine and14C serine incorporation into the proteins. Progesterone is not able to stimulate phosvitin synthesis induction.These studies were supported in part by the Italian CNR grants. 相似文献
12.
B Bernard M Aubery R Bourrillon 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1980,291(2):295-298
The proliferative capacity of Chick embryo fibroblasts was modified by Con A treatment. Con A decreased the growth of fibroblasts from young embryos (8 days), whereas the lectin stimulated the growth of fibroblasts from older embryos (16 days). This differential effect of Con A did not result from changes in cellular permeability to thymidine, but rather from Con A induced modifications of hexose transport. Changes in hexose transport would cause, as a response, parallel modifications in glycolysis and hence energy charge, which would alter proliferative capacity. 相似文献
13.
Summary Chick embryo mesoderm cells were explanted to culture systems in vivo and in vitro and their subsequent movements were correlated with the external morphology as studied by SEM. In vitro cell movements are exaggerations of normal in vivo movements where a 2-dimensional substrate is encountered rather than a 3-dimensional environment.The authors are grateful to Mr J. Smith and Mrs S. Bulman who provided excellent technical assistance and to Mr G. L. C. McTurk who skillfully produced the scanning electron micrographs in the Leicester University Scanning Electron Microscope Unit. 相似文献
14.
H Gérard 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1980,290(2):109-112
By engrafting the chorio-allanto?s of Chick embryos with spleen fragments from homologous adult donors, one elicits a graft-versus-host disease. When the graft is performed before the bursa of Fabricius has reached the lymphoid differentiation state, the final follicular organization is greatly reduced. It seems that this suppression effect cannot be related to a diminution of the stem cell compartment available in the anlage but is a result of a direct impact on the mesenchyme and/or the epithelium of the bursa. 相似文献
15.
Comparative evaluation of 7 helminth antigens in the enzyme-linked immunosorbent assay (E.L.I.S.A.).
112 sera from Europeans with parasitologically proven helminthiasis were tested in the enzyme-linked immunosorbent assay (E.L.I.S.A.) against 6 crude extracts of various helminths (2 of adult worms: Dipetalonema viteae, Fasciola hepatica; 3 of eggs: Ascaris suum, Toxocara canis, Schistosoma mansoni; and of Echinococcus granulosus scolices) and against bovine hydatid fluid. Each serum was tested simultaneously at a fixed dilution of 1:160 against all antigens. Extensive cross-reactions were observed, leading to the conclusion that non-purified helminth antigens, even in combination, are of limited value for reliable serodiagnosis in E.L.I.S.A. 相似文献
16.
Summary 112 sera from Europeans with parasitologically proven helminthiasis were tested in the enzyme-linked immunosorbent assay (E.L.I.S.A.) against 6 crude extracts of various helminths (2 of adult worms:Dipetalonema viteae, Fasciola hepatica; 3 of eggs:Ascaris suum, Toxocara canis, Schistosoma mansoni; and ofEchinococcus granulosus scolices) and against bovine hydatid fluid. Each serum was tested simultaneously at a fixed dilution of 1:160 against all antigens. Extensive cross-reactions were observed, leading to the conclusion that non-purified helminth antigens, even in combination, are of limited value for reliable serodiagnosis in E.L.I.S.A. 相似文献
17.
P Bernard F Couraud 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1979,289(3):315-318
Embryonic Chick hearts aged less than 4 days are not always sensitive to tetrodotoxin, an inhibitor of fast sodium channel. It is shown that in the most frequent cases, in which tetrodotoxin sensitivity is apparently absent, this sensitivity can be demonstrated after previous treatment by veratridine or by toxin II of androctonus australis Hector Scorpion venom. It is concluded that the fast tetrodotoxin-sensitive sodium channel is regularly present in the heart of Chick embryos aged 2 and 3 days, but most often in a permanently inactivated state. 相似文献
18.
J P Blanchet C Doinel 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1978,287(10):963-966
I and i antigens were detected by indirect immunofluorescence on human erythrocytes. 80% of the cord blood cells were brightly fluorescent with anti-i, 15% only with anti-I. In adult blood, this ratio is reversed, 2% only of the cells being intensively labelled with anti-i. The percentages characteristic of adult blood are established within the first 8 months of life. 相似文献
19.
Summary The hypoglycemic agent tolbutamide was tested for its action on the cleavage and differentiation of the sea urchin embryo. Tolbutamide effects a strong selective action on the endoderm which becomes suppressed. 相似文献
20.
Chick blastoderms were studied by scanning and transmission electron microscopy to identify by both methods a type of thread-like structure lying on the epiblast. The structure was identified by transmission microscopy as a long telophase bridge containing mid-body and spindle remnant. It appears to provide cytoplasmic continuity between only 2 cells. 相似文献