蝴蝶兰的离体培养与快繁技术研究

将蝴蝶兰花梗腋芽作为外植体,接种于添加不同浓度激素配比的MS培养基上进行离体培养。实验结果表明,不管是已开花的花梗还是未开花的花梗,其腋芽均能诱导产生不定芽,其适宜的培养基配方为MS 6-BA 5.0mg/L;利用诱导产生的不定芽的茎尖进行茎尖培养,可得到大量的原球茎状体,其适宜的培养基为MS 6-BA 3.0mg/L 10％椰乳 5％马铃薯,其诱导率为65％;原球茎状体进行增殖培养的适宜培养基为KC 5％椰乳 10％马铃薯,其增殖倍数为24.2。     

蝴蝶兰原球茎状体的诱导及增殖

利用蝴蝶兰的植株的不同部位,采用不同培养基对其进行诱导和增殖效果比较。结果表明,香蕉泥和椰胚乳(CM)对于蝴蝶兰外植体原球茎状体的形成具有明显的促进作用。在MS 6 BA5.0mg L NAA2.0mg L 香蕉泥10.0% AC0.3%的培养基中,叶片原球茎状体的诱导率可达48.6%;花梗腋芽和茎尖适宜的诱导培养基配方均为MS 6 BA5.0mg L NAA2.0mg L CM15.0% AC0.3%,其原球茎状体的诱导率分别达72.2%和64.3%。     

利用从芽途径快速繁殖蝴蝶兰的研究

报道了利用从生芽途径快速繁殖蝴蝶兰的研究结果,试验表明:培养基MS+BA1.0mg·L-1+NAA0.05mg·L-1+GA30.5mg·L-1能够诱导花梗休眠芽转化为营养芽.花梗芽在培养基MS+BA5.0mg·L-1+NAA0.05mg·L-1上能够诱导产生丛生芽.在丛生芽增殖阶段,以BA12.5mg·L-1+NAA0.05mg·L-1的增殖效果最好.BA、KT、ZT均能诱导产生"丛生芽",其中以ZT和BA的诱导效果最好.另外,在增殖阶段,外植体采用双芽接较单芽接的增殖效果好.在生根阶段以1 2MS+NAA0.5mg·L-1+w=0.2%的活性炭+φ=10%的椰子水的生根效果较佳,生根率可达100%,且植株生长健壮.试管苗移栽在水苔上的成活率较高,成活率达96 7%.     

激素对不同品种丰花月季组织培养及快速繁殖的影响

丰花月季含腋芽的茎段在 MS NAA0.4 6—BA3.0,pH 为5.8的培养基上可迅速萌发.将已萌发的芽切下,转入 MS NAA0.005 6—BA3.0的继代培养基中,可诱导出大量的丛生芽,再将丛生芽分离,转入生根培养基1/2 MS NAA0.5中生根培养,就形成了发育良好的完整小植株,丰花月季种类繁多,相同配比的激素,对不同的品种影响情况不同.     

雷公藤组织培养快繁技术初探

以带腋芽的幼嫩茎段为外植体，研究雷公藤的离体快繁技术。结果表明：附加2.0mg／L BA和0.1mg／L IBA的MS固体培养基可诱导腋芽萌发，诱导率达95％；附加3．0mg/L BA和O．1mg/L IBA的MS固体培养基为适宜的芽苗继代增殖培养基，增殖率可达5．21。     

大丽花组织培养及快繁技术研究

目的探讨适合大丽花离体快繁和再生的外植体类型和激素配比.方法以D6大丽花品种为试材,比较不同类型外植体愈伤组织和芽的分化情况,以及不同激素配比对腋芽和叶片愈伤组织诱导和分化的影响.结果顶芽和带腋芽的茎段在MS+6-BA 3 mg/L+NAA 0.1 mg/L培养基上容易形成丛生芽;刚萌动的腋芽在MS+6-BA 1 mg/L+NAA 0.2 mg/L培养基上出芽率达到81.3%,平均芽数为3.8;叶片在MS+6-BA 3 mg/L+NAA 0.1 mg/L的培养基上不定芽的分化率为37.5%,平均芽数为4.2.结论顶芽和带腋芽茎段是进行离体快繁最适合的外植体,适合刚萌动的腋芽生长和分化的激素配比是6-BA 1 mg/L+NAA 0.2 mg/L,适合叶片分化不定芽的激素配比是6-BA 3 mg/L+NAA 0.1 mg/L.     

栀子腋芽丛生离体繁殖及开花诱导

以栀子(Gardenia jasminoides Ellis)茎尖和腋芽为外植体进行腋芽丛生离体繁殖以及开花诱导的研究.结果表明:在培养基MS 6-BA 1.0 mg/L IBA 0.2 mg/L中,腋芽丛生效果最好,平均每个外植体可得到8～10个丛生芽,每个茎尖可得到15～20个丛生芽;在培养基MS 6-BA 1.0 mg/L NAA 0.1 mg/L GA3 1.0 mg/L 中,有花蕾的形成,且最终能够开花并形成果实;生根培养基为:1/2 MS IBA 0.5 mg/L GA3 0.5 mg/L,生根率可达98 %,生根培养4周后移栽,移栽成活率达95%.     

花叶竹芋的组织培养

花叶竹芋芽外植体接种到MS附加BA（4mg·L-1），KT（4mg·L-1）或BA（5mg·L-1），KT（5mg·L-1）或BA（10mg·L-1）培养基中，外植体芽继续生长，腋芽萌发．萌发芽继续诱导新芽或转移到生根培养基诱导根，诱导芽转移到MS附加NAA（2mg·L-1）培养基中形成不定根，从而形成完整植株，移植到花盆后形成再生植株．繁殖系数为：8植株／芽·代，由一个芽诱导产生新的植株约需30d。     

黑节铁皮石斛原球茎诱导、再分化以及腋芽萌发和生根的初步研究

对黑节铁皮石斛的原球茎诱导、再分化以及腋芽萌发和生根进行了初步的研究,以期为黑节铁皮石斛的人工繁殖提供理论指导。采用植物组织培养和单因子试验的方法,获得了黑节铁皮石斛带芽茎段最佳的消毒方式为70%酒精处理30s后再用0.1%HgCl2处理20min。黑节铁皮石斛带芽茎段原球茎诱导的最佳培养基为MS＋KT1mg/L＋NAA0.1mg/L。黑节铁皮石斛带芽茎段原球茎再分化的最佳培养基为MS＋KT2mg/L＋NAA0.5mg/L。黑节铁皮石斛带芽茎段腋芽萌发的最佳培养基为MS＋KT2mg/L＋NAA0.5mg/L。黑节铁皮石斛带芽茎段生根的最佳培养基为1/2MS＋NAA0.5mg/L。这些实验结果为黑节铁皮石斛试管苗的工厂化生产奠定了技术基础。     

蝴蝶兰叶片诱导植株再生的研究

以蝴蝶兰叶片为外植体,建立了蝴蝶兰植株再生系统。结果表明:(1)可通过花梗节芽建立丛生芽诱导体系,获得大量无菌材料,丛生芽诱导增殖的最佳激素组合为7.5 mg/L 6 BA 1.0 mg/L NAA;(2)植物激素显著影响叶片类原球体的诱导,在10.0 mg/L 6 BA 1.0 mg/L NAA的激素组合处理下,各品种的诱导效果最好;(3)4种基本培养基中,1/2MS和Hyponex对类原球体的增殖和分化效果较好;(4)蔗糖浓度对类原球茎分化成苗的影响可用y=-0.019 92x2 0.077 51 x 0.17 进行拟合,在蔗糖浓度为20 g/L时,蝴蝶兰类原球茎分化率达最高。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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