Biochemical observations on LSD-25 and deseril

Zusammenfassung Bei Ratten wurde die Verteilung von LSD und Deseril 20 min nach intravenöser Verabreichung fluorimetrisch bestimmt. Auf Grund der ermittelten Quotienten: Gewebe/Blut ergab sich, dass beide Lysergsäurederivate ein gleiches Verteilungsschema in Lunge und Muskel, d. h. in peripheren Organen aufweisen. Im Gegensatz hierzu konnten von Deseril im Gehirn nur ca. 13% des zu erwartenden Wertes nachgewiesen werden. Die Bedeutung dieses Befundes wird diskutiert.     

The effect of LSD-25 upon the cerebral blood flow and EEG in cats

Zusammenfassung Es wurde gezeigt, dass LSD-25 (d-Lysergsäure-diäthylamid) in Dosen von 30 bis 100 /kg Körpergewicht bei dernichtnarkotisierten Katze (encéphaleisolé) meistens cine Erhöhung des zerebralen Gefässwiderstandes herbeiführte. In die Carotis injiziert, gab LSD-25 in denselben Dosen nach vorübergehender, halbseitiger Aktivierung eine Depression des Elektroenzephalogrammes. Kleinere Dosen gaben im EEG eine Aktivierung oder waren ohne Wirkung.     

Testing of rifampicin on possible genetic effects onDrosophila melanogaster and human leukocyte chromosomes in vitro

Zusammenfassung Mutagenitätsuntersuchungen von Rifampicin anDrosophila melanogas (X-chromosomale rezessive Letalmutationen) und an menschlichen Leukozytenchromosomen in vitro ergaben keine Anhaltspunkte für eine genetische Wirksamkeit dieser Substanz.

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We thank Mrs.R. Pieper for her carful technical assistance. We are also grateful to ProfessorR. Hess and Dr.J. Gelzer (Ciba-Geigy, Basel) for the supply of rifampicin.     

Effect of ethanol and isopropanol on the activity of alcohol dehydrogenase,viability and life-span inDrosophila melanogaster andDrosophila funebris

Summary The effects caused by the addition of 2 alcohols to the culture medium of 2 species ofDrosophila, D. melanogaster andD. funebris, are compared. Ethanol at 1% concentration causes slight and tolerable changes in both species. 1% isopropanol is lethal in 1 species and causes drastic changes in the other.The author acknowledge Dr A. Prevost for continuous support and E. Juan and Ll. Serra for their helpful suggestions.     

The syntheses of axillarin and its related compounds

Zusammenfassung Axillarin (5, 7, 3, 4-Tetrahydroxy-3,6-dimethoxyflavon). 5,3,4-Trihydroxy-3,6,7-trimethoxyflavon und 5,7,3,4-Tetrahydroxy-3,8-dimethoxyflavon wurden synthetisiert.     

Effects of photoperiod and temperature on reproductive diapause inDrosophila testacea

Summary In photoperiodic adult diapause ofDrosophila testacea, the critical daylength fell between 14 and 16 h at 15°C, but between 13 and 14 h at 18°C. None entered diapause at LD 1014 and LL at 23°C. This species quantitatively responded to daylengths around the critical daylength in the process of diapause induction. The length of the sensitive period seems to change with temperature.     

Glutamine aminotransferase and glutamine aminohydrolase ratio as a possible test for antitumour compounds.

Three known antitumour drugs have been tested for their effect on the GAT/GNase ratio of Ehrlich Ascites cells and host plasma. It was observed that all these drugs had changed the ratio of the 2 types of glutaminases from below 1.0 to the normal value of 1.0, this was accompanied with an increase in the survival time of the tumour-bearing animals. There was, however, no effect on the plasma GAT/GNase ratio of normal animals in the presence of the 3 antitumour compounds tested.     

A method for injection and transplantation of nuclei and cells inDrosophila eggs

Summary Microinjection ofDrosophila eggs using the principle of thermal expansion is described. The eggs, mounted on cover slips with adhesive, are slightly dried to avoid the formation of exudate and then covered with fluorocarbon oil. Injections are made with a specially designed electrically controlled micropipette.     

A novel oxidative cyclization of 4-arylthiosemicarbazides and related compounds

Résumé Il est démontré que les 4-arylthiosemicarbazides sont oxydés par le brome dissous en chloroforme aux 2-hydrazinobenzothiazoles avec de modestes rendements. Une telle réaction sur les thiosémicarbazones correspondants donne les 1-arylidénè 2-(2-benzothiazolyle)hydrazines avec de très bons rendements. Ceux-ci purent, par la suite, être oxydés par le chlorure de fer⁺³ en solution éthanolique aux 3-aryl-s-triazolo (3,4-b)benzothiazoles. La présence de quelques substituants dans les restes 4-aryl et/ou 1-arylidène dans la moitié thiosemicarbazone favoriserait la cyclisation aux 3:4-diaryl-5-mercapto-1:2:4-triazoles.     

Gender- and site-related effects on lipolytic capacity of rat white adipose tissue

Gender- and site-related differences in the lipolytic capacity, at the different steps of the adrenergic pathway, in gonadal and inguinal white adipose tissue (WAT), were assessed by studying _2A-adrenergic receptor (AR), ₃-AR and hormone-sensitive lipase (HSL) protein levels, and by determining the lipolytic response to different agents. Gonadal WAT showed a lower _2A/₃-AR ratio, a greater lipolytic capacity in response to AR agonists, and higher HSL activity and protein levels than inguinal WAT. In female rats, we found greater _2A-AR protein levels and _2A/₃-AR ratio compared to their male counterparts, but, on the other hand, a higher lipolytic response to -AR agonists and a greater lipolytic capacity at the postreceptor level, including a more activated HSL protein. Thus, the lipolytic capacity was clearly higher in gonadal than in inguinal WAT, at the different steps of the adrenergic pathway studied. Moreover, in both tissues, females showed a greater inhibition of lipolysis via ₂-AR, which was counteracted by the higher lipolytic capacity at the postreceptor level.Received 1 April 2003; received after revision 11 June 2003; accepted 23 June 2003     

The syntheses of 3,8-Dimethoxy-5,7,4′-trihydroxyfIavone and related compounds

Zusammenfassung 3, 8-Dimethoxy-5, 7,4-trihydroxyflavon und 3,6-Dimethoxy-5, 7, 4-trihydroxyflavon wurden synthetisiert.