反相高效液相色谱法同时测定4种苯二氮Chuo类药物

提出了一种反相高效液相色谱法同时测定4种苯二氮Chuo类药物的分析方法。考察了4种苯二氮Chuo类药物的保留值与流动相组成、流速、pH及柱温等色谱条件的关系，优化了色谱条件．确定了以ODS Hypersil为色谱柱，甲醇-水(5l：49；V／V，用氨水调pH7．8)为流动相，流速为0．8mL／min，柱温为45℃，检测波长为220nm的最佳色谱条件,甲苯作为内标物，以内标法峰面积定量,硝西泮，地西泮，三唑仑和艾司唑仑线性范围分别是0．1mg／L～320mg／L，0．2mg／L～320mg／L，0．1mg／L～320mg／L和0．08mg／L～320mg／L．检测限分别为0．1mg／L，0．2mg／L，0．1mg／L和0．08mg／L,该方法用于尿液中的苯二氮革类药物的测定,其回收率为97．0％～108．7％，标准偏差为1．7％～3．0％(n=7),该方法简单、快速、精确、灵敏、重复性好。     

大孔径毛细管气相色谱法快速测定饮料中的普鲁卡因

采用固相萃取技术，以二十二烷为内标，FID检测器检测，建立了一种测定饮料中普鲁卡因的大孔径毛细管气相色谱方法，测定结果表明普鲁卡因检测的线性范围为ρ(普鲁卡因)=0．04mg／L～64mg／L，其最低检测质量浓度为0．04mg／L，相对标准偏差为2．6％，平均回收率为85．6％。     

何首乌茎段离体培养的研究

用何首乌种子经表面消毒后进行无菌发芽并建立了无菌材料，以无菌实生苗茎段作外植体进行离体快繁研究。试验了不同基本培养基(MS，H，1／2MS，B5和N6)，不同种类外源激素(IBA、NAA、KT)和不同浓度梯度的外源激素(0．0mg／L，0．1mg／L，0．Smg／L，1．0mg／L，2．0mg／L和4．0mg／L)以及不同蔗糖浓度(0．0％，1．0％，2．0％，4．0％，8．0％，16．0％)对茎段离体培养的影响。结果表明：MS基本培养基，0．5mg／L IBA(或0．5mg／LNAA)及3％的蔗糖比较适合于何首乌的茎段离体快繁。推荐用于何首乌茎段离体快繁的最佳培养基配方为：MS 肌醇100mg/L^ 盐酸硫胺素1．0mg／L 盐酸吡哆素0．5mg／L 甘氨酸2．0mg／L IBA0．5mg／L(或NAA0．5mg／L) 蔗糖3．0％ 琼脂0．75％，pH5．9。用该培养基对何首乌进行茎段离体快繁，每次继代培养可增殖4～5倍。     

新疆鸡冠花食用色素粗成分与有害微量元素含量的测定

本以自制新疆鸡冠花(Celosia cristata L．)花食用色素为实验材料，分别对色素中的灰分、总糖、粗蛋白、粗脂肪、氨基酸总量和有害微量元素AS、Pb、Hg和Ca的含量进行了检测。结果表明：色素中各种成分的含量分别为：灰分18．6％，总糖l.06％，粗蛋白5.16％，粗肪脂0．13％，氨基酸总量5．08％；有害微量元素含量分别为：As0．033mg／kg，Pb0．038mg／kg，Hg0．18mg／kg，Cu0．061mg／kg。     

斑节对虾白斑综合症暴发流行与水体理化因子的关系

用海水化学分析方法每天检测5口斑节对虾（Penaeus manodon）养成池两茬共90余天9项水质指标,结果表明它们的变化范围是：温度14．7－31．8℃、盐度13．6－30．6、溶解氧2．3－9．8水质指标,结果表明它们的变化范围是：温度14．7－31．8℃、盐度13．6－30．6、溶解氧2．3－9．8mg／L、pH7．3－9．5、硫化物（S^2-）0－0．13mg／L、磷酸盐（PO4^3-P）0－2．33mg/L、硝酸氮（NO3－N）0－1．33mg／L、亚硝酸氮（NO2^2-N)0．0．28mg／L、氨态氨（NH4^ -N NH3-N)0-0.66mg/L, 皆在斑节对虾该项指楠的耐受范围内,且无论这些因子含量如何变化,5口虾池两茬在虾养殖55d后皆因患WSS而死亡,表明在所检测的虾池这些水质因子并非WSSV感染暴发流行的指标因子。     

野大麦组织培养及植株再生的研究

以野大麦成熟胚为外植体，接种于MS 2，4-D2．0mg／L KT0．2mg／L NAA0．5mg／L Su3％的培养基中，诱导愈伤组织；然后在培养基MS 2，4-D2．0mg／L KT0．2mg／L NAA1．0mg／L Su3％上进行继代培养，每次继代时间为20天左右；再用MS KT2．0mg／L NAA0．2mg／L Su1．5％进行分化，分化率达80％以上．     

三种保鲜剂对延缓须苞石竹切花衰老的影响

对须苞石竹切花瓶插期三种不同保鲜剂效果进行了对比实验，以蒸馏水为对照，测定了花瓣衰老过程中的含水量、总糖量和可溶性蛋白含量，结果显示，保鲜剂处理的其含量均有明显增加，尤其以自制配方Ⅱ(3％S 250mg/L8—HQ 500mg／L苯甲酸钠 25mg/LAgNO3 25mg/L EDTA-Na2)和Ⅰ号(4％S 500mg／L CA 200mg／L8—HQ 0．02％CaCl3 0．02％KCl)的效果最好，可延缓切花衰老6天--8天。     

镍冶炼废水处理方法

在镍冶炼过程中产生大量的废水，其中含有镍、铜、钴等金属离子．这种冶炼废水在排放前必须加以处理，以便回收有价金属元素和防止污染环境．作者采用中和－混凝法处理镍冶炼废水，并对方法进行了热力学分析，对混凝机理进行了研究．理论计算结果表明：Ni2＋，CU2＋，Co2＋开始沉淀的pH值分别为7．47，6．00和7．68；若沉淀到1mg／L以下时，所控制的pH值分别为8．48，7．01和8．70．通过实验获得的最佳条件为：pH＝9．0，聚合硫酸铁用量为镍含量的2倍，聚丙烯酰胺用量为4mg／L；处理后，废水中ρNi＜0．13mg／L，ρCo＜0．01mg／L，ρCu＜0．005mg／L．     

叶子花的组织培养及快速繁殖研究

以叶子花的茎尖为外植体，通过13种培养基的试验，从中筛出适宜叶子花组织培养、快速繁殖的一整套稳定、高效的生产程序，即芽诱导培养基为MS BA0.2mg／L IAA1．0mg／L，增殖培养为MS BA0.5mg／L IAA1.5mg／L，生根培养基为MS IBA0．1mg／L 2，4，5-T0．1mg／L。为规模化生产叶子花提供了快速繁殖的方法。     

宜兴百合的组织培养和快速繁殖

以组织培养获得的宜兴百合小鳞茎为试验材料，研究了不同基本培养基、NAA与6-BA的配比、矮壮素（CCC）、2，4-D、活性炭、蔗糖及悬浮培养方法对小鳞茎增殖和膨大的影响．结果表明，最佳诱导培养基为MS培养基，有利于提高分化率的最佳配方为：MS＋0．15mg／LNAA＋2．0mg／L6-BA，小鳞茎膨大增重的最佳培养基为：MS＋0．15mg／LNAA＋2．0mg／L6-BA＋0.1mg／L CCC＋6—8％蔗糖，培养方式为液体振荡培养优于固体培养．     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that