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1.
Isolation and characterization of soybean NBS analogs 总被引:1,自引:0,他引:1
Isolation of plant resistance genes is greatly helpful to crop resistance breeding and the insight of resistance mechanism.
The cloned plant resistance genes are classified into four classes according to their putative structural domain, of which
the majority possesses nucleotide-binding site (NBS) domain that consists of P-loop, kinase2a and kinase3a. The conservation
of this domain affords the potential possibility of cloning the plant resistance genes, which is homology-based cloning technique.
In the present study, the degenerate oligonucleotide primers were designed according to the tobaccoN andArabidopsis RPS2, and 358 clones were isolated from the genomic DNA of resistance soybean cultivar Kefengl, resistant to soybean mosaic virus,
and 4 open-reading NBS analogs were finally characterized and designated asKNBS1, KNBS2, KNBS3 andKNBS4. Southern hybridization suggested that they were present with multicopy in the soybean genome;KNBS4 was mapped to F linkage group andKNBS2 co-located J linkage group with the SCAR marker ofRsa resistant to soybean mosaic virus by RFLP analysis. Northern analysis suggested thatKNBS2- related sequence was low and constitutively expressed in the root, stem and leaves of soybean. The detailed characterization
of NBS analogs is very helpful to ultimately cloning the soybean resistance gene. 相似文献
2.
Fine mapping of the rice bacterial blight resistance gene Xa-4 and its co-segregation marker 总被引:1,自引:0,他引:1
Wenming Wang Yongli Zhou Guanhuai Jiang Bojun Ma Xuewei Chen Qi Zhang Lihuang Zhu Wenxue Zhai 《科学通报(英文版)》2000,45(19):1779-1782
An F2 population developed from theXa-4 near isogenic lines, IR24 and IRBB4, was used for fine mapping of the rice bacterial blight resistance gene,Xa-4. Some restriction fragment length polymorphism (RFLP) markers on the high-density map constructed by Harushima et al. and
the amplified DNA fragments homologous to the conserved domains of plant disease resistance (R) genes were used to construct
the genetic linkage map around the geneXa-4 by scoring susceptible individuals in the population.Xa-4 was mapped between the RFLP marker G181 and the polymerase chain reaction (PCR) marker M55. The R gene homologous fragment
marker RS13 was found co-segregating withXa-4 by analyzing all the plants in the population. This result opened an approach to map-based cloning of this gene, and marker
RS13 can be applied to molecular marker-assisted selection ofXa-4 in rice breeding programs. 相似文献
3.
Huafei Lü Xiaotian Ming Lijia Qu Meihua Liu Jing Li Hongya Gu Zhangliang Chen 《科学通报(英文版)》2000,45(3):242-246
The promoter fragments of wheatGstA1 and potatoGst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5′ untranslated leader sequence together withGUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transferred into rice in order to analyze their inducibility
patterns in transgenic rice plants. The results show that: WGA and PGA are both inducible by elicitors ofPyricularia oryzae in transgenic rice cells; the intron I of riceAct1 gene is important for the heterogenic expression of monocot and dicot promoter elements in rice; and theAct1 minimal promoter and its 5′ untranslated leader sequence produced low level background expression in rice. 相似文献
4.
Cloning and analysis of expression profile of 13<Emphasis Type="Italic">WRKY</Emphasis> genes in rice 总被引:15,自引:0,他引:15
QIUYuping JINGShaojuan FUJian LILu YUDiqiu 《科学通报(英文版)》2004,49(20):2159-2168
5.
Li Li-jia Song Yun-chun Key Laboratory of MOE for Plant Developmental Biology Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2003,8(4):1167-1172
0 IntroductionMaizeisamongthemostintensivelystudiedspeciesingeneticsandoneofagronomicallythemostimportantplants.Therearemanydis easemicrobesandpeststoattackmaize,whichre sultsinlowproductionandbadquality .Withthedevelopmentofverydensegeneticmapconstruc tion ,avarietyoftheimportantdiseaseresistancegenesofmaizeincludingHelminthosporiumtur ciumPassresistancegenesHt1,Htn1andHt2 ,HelminthosporiummaydisNisikresistancegenesRhm1andRhm2 ,maizedwarfmosaicvirusresis tancegeneMdm1,wheatstreakmosaicvi… 相似文献
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YU Jun HU Songnian WANG Jun LI Songgang WONG Ka-Shu Gane LIU Bin DENG Yajun DAI Li YUAN Longping YANG Huanming 《科学通报(英文版)》2001,46(23):1937-1942
The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development,
and evolution, as well as information on many beneficial phenotypes of economic significance. Using a “whole genome shotgun”
approach, we have produced a draft rice genome sequence ofOryza sativa ssp.indica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over
4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant
sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb
from anindica variant cultivar93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage
of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEMBLER, a custom-designed
software package used for the initial assembly, were verified rigorously by comparisons against finished BAC clone sequences
from bothindica andjapanica strains, available from the public databases. Over all, 96.3% of full-length cDNAs, 96.4% of STS, STR, RFLP markers, 94.0%
of ESTs and 94.9% unigene clusters were identified from the draft sequence. Our preliminary analysis on the data set shows
that our rice draft sequence is consistent with the comman standard accepted by the genome sequencing community. The unconditional
release of the draft to the public also undoubtedly provides a fundamental resource to the international scientific communities
to facilitate genomic and genetic studies on rice biology.
These authors contributed equally to this work. 相似文献
9.
Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed fromOryza eichingeri 总被引:2,自引:0,他引:2
Guoqing Liu Huihuang Yan Qiang Fu Qian Qian Zhitao Zhang Wenxue Zhai Lihuang Zhu 《科学通报(英文版)》2001,46(17):1459-1462
Wild rice species is an important source of useful genes for cultivated rice improvement. Some accessions of Oryza eichingeri (2n = 24, CC) from Africa confer strong resistance to brown planthopper (BPH), whitebacked planthopper (WBPH) and bacterial blight (BB). In the present study, restriction fragments length polymorphism (RFLP) and simple sequence repeats (SSR) analysis were performed on disomic backcross plants between Oryza sativa (2n = 24, AA) and O. eichingeri in order to identify the presence of O. eichingeri segments and further to localize BPH-resistant gene. In the introgression lines, 1—6 O. eichingeri segments were detected on rice chromosomes 1, 2, 6, or/and 10. The dominant BPH resistant gene, tentatively named Bph13(t), was mapped to chromosome 2, being 6.1 and 5.5 cM away from two microsatellite markers RM240 and RM250, respectively. The transfer and localization of this gene from O. eichingeri will contribute to the improvement of BPH resistance in cultivated rice. 相似文献
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以"浦软粳S"为转育亲本,利用分子标记辅助常规育种技术成功培育出含有Pi9,Pita,Pib和Pigm稻瘟病抗性基因及软米基因(Wx~(mq)),同时表现柱头外露率高的两系不育系水稻新品系"2179S"."2179S"不育系茎秆粗壮,矮杆大穗,株高为63.8 cm,柱头外露率平均为60%.研究结果为今后培育具有稻瘟病抗性的优质两系杂交水稻新组合提供不育系亲本. 相似文献
12.
A brown planthopper (BPH) resistance line, B5, derived its resistance genes from the wild riceOryza officinalis Wall exwatt, was hybridized with Taichung Native 1, a cultivar highly susceptible to BPH. A mapping population composed of
randomly selected 167 F2 individuals was used for determining the BPH resistance genes by the restriction fragment length polymorphism analysis (RFLP).
Bulked segregant analysis was conducted to identify RFLP makers linked to the BPH resistance genes in B5. The results indicated
that the markers linked to BPH resistance are located at two genomic regions on the long arm of chromosome 3 and the short
arm of chromosome 4, respectively. The existence of the two loci was further assessed by the quantitative trait locus (QTL)
analysis. We located the two loci at a 3.2 cM interval between G1318 and R1925 on chromosome 3 and a 1.2 cM interval between
C820 and S11182 on chromosome 4. Comparison with the BPH genes that have been reported indicated that the BPH resistance genes
in B5 are novel. These two genes may be useful BPH resistance resource for rice breeding. Furthermore, the mapping of the
two genes is useful for cloning the BPH resistance genes. 相似文献
13.
A novel tobacco gene coding for a product similar to bacterial two-component regulators 总被引:5,自引:0,他引:5
The two-component signaling system has been studied in bacteria. It takes part in signal transduction of adaptive behavior.
Recent studies have shown that a similar two-component system is also present in eukaryotes. Examples of this areETRl andCKLl genes which may involve the signal transduction of plant hormone ethylene and cytokinin respectively. The cloning and characterization
of a novel gene (NTHKl) fragment from tobacco are presented. Its partial sequence codes for a product which shows similarity to many two-component
signaling proteins. Southern blot analysis indicated that there are 2 to 3 copies ofNTHKl gene in tobacco genome (allotetraploid). Homologous genes may also exist in other plants such as Arabidopsis, soybean and
spinach. The expression ofNTHKl gene has also been analyzed in tobacco. Further studies on the isolation of full-length cDNA ofNTHKl gene will elucidate more clearly its function in signal perception and transduction. 相似文献
14.
利用分子标记检测技术,对9种参加2020年上海市水稻区域试验的品种和2种本课题组新培育的新品系的共10个抗稻瘟病基因位点进行了检测.结果显示,Pi37,Pi41,Pi-d23个基因在11种水稻中出现的频率达100%,Pi2,Pi5,Pi9,Pi36,Pikm和Pib抗性基因在11种水稻中出现的频率分别为18.18%,9... 相似文献
15.
Rice (Oryza sativa L.) eating quality is one of themost important traits. Amylose content (AC) in rice en-dosperm is a major index affecting rice eating quality[1,2].It has a negative correlation with gel consistency of rice[3].Based on amylose content, r… 相似文献
16.
Using the primers which specially amplify the conservative motif of Human SRY gene, we studied the PCR amplification of Sox
genes in genomic DNA of two species of mud loach:Misgurnus anguillicaudatus andParamisgurnus dabryanus. Four bands with the length of 200,550,940 and 1000 bp respectively, were presented in the PCR products ofMisgurnus anguillicaudatus. Three bands with the length of 200,550 and 900 bp were presented in that ofParamisgurnus dabryanus. Southern blotting results indicated that the 200 and 550 bp bands are specially positive. There is no difference between
male and female individuals as well as between these two species.
Chang zhongjie: Born in Nov. 1965, Ph. D. graduate student 相似文献
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根据已经克隆的植物抗病基因和候选抗病基因的保守序列P-loop、Kinase-2及GLPLAL设计一系列简并引物,利用同源序列扩增法,对玉米的基因组DNA进行PCR扩增,并对5个扩增产物的克隆进行测序.测序结果在Gen—Bank内进行BLAST检索,发现A9克隆序列与玉米BAC库中的206C17克隆的部分序列有很高的相似性,并且距离GenBank内注册的玉米抗锈病基因rpl位点中的rpl-3基因、rpl-4基因分别约有66Kb、20Kb,且A9克隆序列在玉米基因组中是单拷贝的.这为玉米抗锈病性状的分子标记辅助选择和抗锈病基因的克隆奠定了良好的基础。 相似文献
18.
根据U2nRNA基因的保守性和结构特点设计引物,通过PCR法扩增U2snRNA连锁基因间区的DNA片段并进行顺序分析,证实在水稻中至少存在一组U2snRNA连锁基因。对水稻总DNA的PCR分析结果表明,水稻中可能还存在其他形式的连锁基因。PCR方法可以有效地应用于结构保守基因的连锁分析中。 相似文献
19.
用基因枪法将AGP基因导入水稻的初步研究 总被引:1,自引:1,他引:0
以水稻成熟种子的愈伤组织为受体,采用基因枪法将AGP基因导入水稻细胞,通过组织培养和抗性筛选,得到了转基因植株,转基因植株总DNA的PCR分析初步表明,目的基因已整合到水稻的基因组中。 相似文献
20.
Xiuhua Chen Qiaoquan Liu Zongyang Wang Xingwen Wang Xiuling Cai Jingliu Zhang Minghong Gu 《科学通报(英文版)》2002,47(14):1192-1197
Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality
ofindica hybrid rice is closely related to the high amylose level in rice grains. In order to improve the grain quality of theindica hybrid rice by genetic engineering, an antisense fragment of ricewaxy gene, driven by the 5′-franking sequences of the ricewaxy gene, was successfully introduced into three major parent lines ofindica hybrid rice, all contain a high amylose level in the grains, viaAgrobacterium, and more than 100 hygromycinresistant plants were regenerated. The analysis of PCR amplification and Southern blots indicated
that the T-DNA containing the antisensewaxy gene had been integrated into the genome of transgenic rice plants. Most of the primary transgenic rice plants grew normally,
and the mature seeds from these transgenic plants were performed for analysis of the amylose content. The results showed that
the amylose content in the endosperm of some grains was reduced and the lowest reached 7.02% in one homozygous transgenic
line, 72.4% lower than that of the wild type. The influence of the altered amylose content on the gelatinization temperature
and gel consistency was also observed in several homozygous transgenic rice plants.
The two authors contributed equally to this work. 相似文献