Hormonal induction of steroid sulphatase in the mouse

Summary By comparing steroid sulphatase levels per se, and also ratios to -galactosidase, in 6 sets of mice — normal females, entire and castrated males both with and without exogenous testosterone administration — we obtained support for the contention that induction of this enzyme is in part controlled by male hormones.     

Hormonal induction of glutamate dehydrogenase in rat liver

Summary Glutamate dehydrogenase rapidly increases in microsomes and appears in the cytoplasm after administration of cortisone, cAMP, hydrocortisone-acetate. Prolonged administration of ACTH maintains high level of enzyme in the mitochondria and microsomes. Hydrocortisone-acetate, insulin and corticosterone decrease drastically enzyme in mitochondria.Supported by N.I.H. grant Nr. PR 0806-02.     

Hormonal control of manganese transport in the mouse thyroid

The present study deals with a possible mechanism controlling the transport of manganese (Mn), an essential trace element, from the circulation to the thyroid. Mice were pretreated with propylthiouracil (PTU) or triiodothyronine (T₃), and a measurement of the thyroid:serum concentration ratio (T/S) of radioactive manganese (⁵⁴Mn) was carried out. The T/S of⁵⁴Mn was greatly enhanced by PTU, but reduced by T₃. Several methods were used to demonstrate that the T/S of⁵⁴Mn depends upon the level of thyroid-stimulating hormone (TSH) in the serum. First, bovine TSH was injected into mice; an increase in the T/S resulted. Secondly, serum thyroxine and T₃ levels measured by radioimmunoassay (RIA) suggested that PTU produced an increase in serum TSH and T₃ a decrease. However, direct measurement of mouse TSH by RIA for rat TSH failed to produce proof of any changes in TSH level, owing to poor cross-reactivity. Taking all the information into account, it is concluded that Mn-transport into the thyroid is controlled by the thyroid state.     

Hormonal induction of glutamate dehydrogenase in rat liver.

Glutamate dehydrogenase rapidly increases in microsomes and appears in the cytoplasm after administration of cortisone, cAMP, hydrocortisone-acetate. Prolonged administration of ACTH maintains high level of enzyme in the mitochondria and microsomes. Hydrocortisone-acetate, insulin and cortisone decrease drastically enzyme in mitochondria.     

Hormonal control of manganese transport in the mouse thyroid.

The present study deals with a possible mechanism controlling the transport of manganese (Mn), an essential trace element, from the circulation to the thyroid. Mice were pretreated with propylthiouracil (PTU) or triiodothyronine (T3), and a measurement of the thyroid:serum concentration ratio (T/S) of radioactive manganese (54Mn) was carried out. The T/S of 54Mn was greatly enhanced by PTU, but reduced by T3. Several methods were used to demonstrate that the T/S of 54Mn depends upon the level of thyroid-stimulating hormone (TSH) in the serum. First, bovine TSH was injected into mice; an increase in the T/S resulted. Secondly, serum thyroxine and T3 levels measured by radioimmunoassay (RIA) suggested that PTU produced an increase in serum TSH and T3 a decrease. However, direct measurement of mouse TSH by RIA for rat TSH failed to produce proof of any changes in TSH level, owing to poor cross-reactivity. Taking all the information into account, it is concluded that Mn-transport into the thyroid is controlled by the thyroid state.     

The kinetics of sulphatase A

Résumé Les cinétiques de l'hydrolyse du sulfate de nitrocatéchol par la sulfatase A sont très anormaux parce qu'il n'y a aucune relation linéaire entre la vitesse de la réaction et la concentration de l'enzyme. La réaction tient trois phases: dans la première, la vitesse décroît rapidement, dans la deuxième, elle diminue de plus en plus tandis qu'elle s'accroît dans la troisième. La réaction est très sensible à la composition du mélange réactionel, et l'addition des produits de la réaction, particulièrement le sulfate inorganique, augmente la vitesse de l'hydrolyse. En utilisant comme substrat lep-nitrophénylsulfate, ces irrégularités ne sont pas constatées.Une interprétation de ces résultats est présentée!     

Phenobarbital liver microsomal induction in MHV-3 viral hepatitis of the mouse

Riassunto Sono state studiate le variazioni di alcuni enzimi microsomiali del fegato di topi trattati con fenobarbital in varie fasi della epatite da MHV-3. È stato osservato che anche in fasi di avanzata citonecrosi epatica il trattamento con fenobarbital determina netti incrementi del citocromo P-450, della NADPH ossidasi e della anilina idrossilasi microsomiali. Nelle stesse condizioni invece la glucosio-6-fosfatasi mostra un decremento sia nei topi controllo che negli infetti.     

Bone induction evoked in mouse by xenogeneic grafts of the transitional epithelium

Résumé Des épithéliums de muqueuse vésicale de chien, de cobay et de hamster ont été greffés sur des souris préalablement traitées à l'acétate de cortisone. L'induction par ces greffes d'un cartilage et d'un tissu osseux prouve que l'inducteur d'ossification formé et libéré par ces épithéliums manque de spécificité générique. On a montré que le faible pourcentage de l'induction d'ossification est imputable à la faible capacité inductive de cet épithélium et non au manque de sensibilité du tissu conjonctif induit de la souris dans le système allogénique.

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We thank MissS. Skaryska, Mrs.E. Ruszkowska and Mr.T. Grabiski for technical assistance and Mr.B. Przybysz for preparing photographs.     

Hormonal induction of ovulation and spawning in the blind cave fish,Anoptichthys jordani with the sue of human chorionic gonadatropin

Zusammenfassung Experimentell wird gezeigt, dass die Injektion von menschlichem Choriongonadotropin beim HöhlenfischAnoptichthys jordani, das Fortpflanzungsverhalten vermehrt und der Ertrag an entwicklungsfähigen Eiern grösser ist, wenn mehrere Pärchen gleichzeitig mit Hormon behandelt und im gleichen Aquarium zur Eiablage belassen werden.     

The induction of tolerance in hamsters to transplants of mouse sarcoma by means of heteroparabiosis

Zusammenfassung Durch die Heteroparabiose Maus-Hamster wurde bei den Hamstern Toleranz auf Crockers Maussarkoma hervorgerufen. Der induzierende Einfluss der Heteroparabiose konnte nur dann beobachtet werden, wenn bei den parabiotischen Mäusen die vorher implantierte Geschwulst gut entwickelt war. Die heterotransplantierte Geschwulst zeigte ein rasches Wachstum nur dann, wenn Milzhomogenat einer geschwulsttragenden Maus in parabiotische Hamster injiziert worden war.     

Hormonal manipulation of carrageenin-induced pyresis in rats

Summary The intensity of the hyperthermic response in rats promoted by subplantar injection of 1 mg of carrageenin is directly related to the irritant properties of the type of carrageenin. The overall pyretic response is more dramatic in female rats than in male rats. Subtle changes in the time-course hyperthermic profiles are seen after hormonal modifications.This work was supported by NIH, MBS research grant No. RR08111.     

Hormonal control of sexual receptivity in cockroaches

Many animals exhibit specific behaviors associated with sexual receptivity only when they are reproductively competent. In insects with gonadal maturation cycles, these behaviors usually coincide with ovarian matruation. In the cockroachBlattella germanica, juvenile hormone (JH), produced by the corpora allata (CA), regulates female reproductive physiology. Various experimental manipulations, including ablation of the CA, therapy with JH analogs, CA denervation, ovariectomy, and changing nutrient quality, coupled with time-lapse video recording, support the hypothesis that JH also controls female sexual receptivity. A re-examination of the role of the CA in the maturation of male sexual readiness shows that, while sexual behavior develops in the absence of JH in bothB. germanica andSupella longipalpa, JH accelerates the expression of sexual readiness.