On the life-habits and developmental stages of Nidomyia cana Papp (Diptera,Borboropsidae)

Eggs, second and third instar larvae of the enigmatic species Nidomyia cana, which was discovered most recently from the nests of raptorial birds in southern Hungary, are described. Both egg and larvae show peculiarities among the so-called heleomyzoid flies. Larvae were found in wet vegetable material (mostly pieces of poplar bark) of a buzzard nest and their shape is of a saprophagous type. Females are macro-oviparous, with a maximum of 15 to 17 eggs laid in one batch. Adults live mostly on the surface the of the nest, they copulate there, and they find shelter in the nest itself. No direct contact with adults or nestlings within the nest was observed. It is suggested that overwintering occurs as pupae (pharate adults) underground, beneath the nest. However, if N. cana lives exclusively in the nests of raptors, adults would have difficulties in finding a new nest, and it is probable that they are transferred to new nests attached to the birds.     

Immature stages of Chinavia musiva (Berg, 1878): a unique pattern in the morphology of Chinavia Orian, 1965 (Hemiptera,Pentatomidae)1

Specimens of Chinavia musiva were collected in Parque Estadual do Espinilho (Barra do Quaraí County, RS, Brazil). Insects were reared under controlled conditions (24±1°C; RH 70±10%; 12h L:12h D), and fed on green beans (Phaseolus vulgaris L.). Eggs of C. musiva differ from the pattern observed in other species of the genus due to chorion colour and surface, and aero‐micropylar processes, number and shape. Exclusive features of the species in the nymphal stage are head and thorax dull; variegated pattern of abdomen, and openings of the scent glands in conspicuous tubercles. Developmental time was, on average, 52 days; females laid more frequently egg masses with 14 or 42 eggs. Females collected from the field as nymphs exhibited higher reproductive performance than females reared on green beans as nymphs and adults, suggesting that wild hosts could be essential for the species life cycle.     

Pre-ovipositional and ovipositional behaviour of Lasioseius ometes (Oudemans) and Hypoaspis kargi Costa (Acari: Dermanyssiae: Ascidae,Laelapidae) with notes on egg protection strategies in Mesostigmata

Egg deposition by the soil mites Lasioseius ometes (Oudemans, 1903) and Hypoaspis kargi Costa, 1968 was observed using continuous video-monitoring. The process in both species was found to consist of four distinct phases. In Phase 1, both species engage in pre-ovipositional activity prior to the egg being extruded from the genital opening. In L. ometes, the female often selects a substrate depression for egg laying and examines the depression carefully with the gnathosoma and legs I before oviposition. In H. kargi, egg deposition sites are chosen without regard for substrate depressions. Phase 1 lasted approximately 3 minutes for both species. Phase 2 involved egg extrusion and holding of the egg beneath the gnathosoma. Phase 2 lasted approximately 20 times longer in L. ometes (5 minutes) than in H. kargi. Alteration of the egg surface in the form of thorn-like outgrowths was recorded in L. ometes. Egg deposition occurs in Phase 3, and in L. ometes the egg is generally placed at the deposition site using the chelicerae. Hypoaspis kargi also uses the chelicerae for egg manipulation, but also adjusts orientation of the egg with legs I. The sticky nature of egg surface in H. kargi may provide a means for the female to effectively cover the exposed egg with a protective layer of soil particles. Egg deposition in both species occurred in less than 90 seconds. Phase 4 involves egg covering, but behaviour of the female during this last phase differs markedly between the two species. In L. ometes, the female tends to remain close to the egg, whereas in H. kargi the female moves away from the egg to search for substrate particles appropriate for its covering. The average time for Phase 4 in H. kargi was approximately four times longer (80+ minutes) than in L. ometes.     

Chorion surface ultrastructure of loach Misgurnus anguillicaudatus: adaptation to the environment and correlation with the reproductive strategy

The ultrastructure of the egg chorion surface in the loach Misgurnus anguillicaudatus was investigated using scanning electron microscopy (SEM). A single micropyle, which belonged to type II, was found at the animal pole of an M. anguillicaudatus egg. Several sinistral grooves which originated at the micropyle probably served for sperm guidance. The egg envelope had numerous pores with bud-like fillings and a regular arrangement like honeycomb. Lots of small knobs were situated on the zona radiata at the vegetal pole. Water contact triggered egg stickiness within about 10 seconds. The egg surface became smooth after treatment with trypsin solution. The features of the micropyle, pores and stickiness were correlated with the reproductive strategy of the loach and are important for egg identification.     

The developmental sequence of Argulus foliaceus (Crustacea: Branchiura)

We describe and illustrate the complete development of Argulus foliaceus L. A metanauplius is followed by nine juvenile stages. Eggs were detected in the thorax of the female at the 11th stage. Comparisons are made between the development of A. foliaceus and the other freshwater species of Argulus currently found in the UK.     

Schistosoma curassoni Brumpt, 1931 in sheep and goats in Senegal

A survey of 5722 sheep and 1752 goats at the abatoir in Dakar, Senegal showed that the overall prevalence of schistosomiasis was 2·1%. Of the 112 animals where identification of the schistosome species was possible, all were infected with Schistosoma curassoni, and 2·7% had mixed infections with S. bovis. The adult worms of S. curassoni are described, and on the basis of egg morphology this species is shown to be distinct from S. bovis and S. mattheei. Eggs of S. curassoni measured 146 μm ± 16·8 × 63·3 μm ± 4·5 from sheep and 149·4 μm ± 13·2 × 62·8 μm ± 4·9 from mouse liver, and appear to be indistinguishable from the eggs of S. haematobium Guede Chantier, Senegal which measure 153·1 μm ± 11·1 × 62·4 μm ± 12·1 from mouse liver. However, S. curassoni differs from S. haematobium in that it develops more quickly than S. haematobium in hamsters, uterine eggs becoming visible at least 20 days earlier; the adult worms of S. curassoni are nearly double the size of S. haematobium in hamsters at 70 days post infection.

Also, S. curassoni develops well in sheep and cattle unlike S. haematobium, but will not develop to maturity in baboons like S. haematobium.     

Pattern of shell occupation by the hermit crab Pagurus exilis (Anomura,Paguridae) on the northern coast of São Paulo State,Brazil

The purpose of this study was to determine the shell occupation pattern of Pagurus exilis using the percentage of shell types that were occupied and the morphometric relationship between hermits and occupied shells. Specimens were collected monthly from July 2001 to June 2003. A total of 1737 individuals were collected, occupying 19 gastropod shell species. Buccinanops gradatum (82%) was the most occupied shell, followed by Natica isabelleana (6.4%), Stramonita haemastoma (3.2%), and Cymatium parthenopeum (2.2%). There was a differential shell utilization pattern between sexes; males occupied larger and heavier shells while the non‐ovigerous females occupied the lightest ones. Pagurus exilis occupied more bare shells instead of incrusted ones. Buccinanops gradatum was the most occupied shell species as a function of its availability in the survey, and this occupation is strongly associated with the higher internal volume presented by these shells.     

On the status of Arcitalitrus dorrieni (Crustacea: Amphipoda) on the island of Colonsay,Inner Hebrides

The presence of the landhopper Arcitalitrus dorrieni (Hunt) is reported on the island of Colonsay, Inner Hebrides. It is widely distributed there amongst deciduous leaf litter in forested areas but also occurs in bracken litter and scrub grassland. The species is an introduction from Australia, doubtless transported inadvertently among plants brought to the gardens at Colonsay House. It is, however, not restricted to the ornamental plantings but has colonized native woodland. Maximum population density recorded under leaf litter was 680 m². Amphipods avoid waterlogged or dry situations preferring to burrow in moist soil under litter from deciduous trees. Coniferous woodlands are more sparsely colonized. Rate of spread (minimum) from the original locus is calculated at c. 25 m/year. Arcitalitrus may prefer to eat sycamore leaves (cf. oak, beech). Ovigerous females are reported for May but no evidence exists for a release of young any earlier in the year. Immature amphipods seem to live more covert lives in the soil beneath the superficial litter layer. Eggs are relatively large and few in number. Amphipods may avoid ants but may have a mite living in phoretic or ectoparasitic association.     

Field studies of Cybocephalus nigriceps nigriceps (J. Sahlberg) (Coleoptera: Cybocephalidae) in Israel

Summary

Cybocephalus nigriceps nigriceps (J. Sahlberg) reproduces in Elat (the Arava region of Israel) during spring and summer. Eggs and larvae are present on date palms during these seasons only, whereas adults occur in the grove throughout the year. The adult population reaches its peak in mid-summer and decreases considerably during spring and autumn. Two main locations in which adults occur during the seasons, were found on date palm trees.

Due to the lack of reproduction and activity during autumn and winter, the predator takes almost no part in the biological control of the parlatoria date scale in these seasons.     

New insights on the external features of egg capsules and embryo development in the squid Loligo vulgaris

The embryonic development of the squid Loligo vulgaris was observed from 183 egg masses collected from special devices deployed throughout Cabrera National Park (Baleares Islands, western Mediterranean Sea). Sequence alignment analysis of the cytochrome oxidase I gene revealed that all embryos belonged to L. vulgaris. In total, 549 egg capsules were examined. Viable egg capsules (n = 420) were classified into one of five maturation stages according to the primary external features. The length of the viable egg capsules varied between 40 and 170 mm, and increased with embryonic development. The non-viable capsules (n = 129) were categorized into four groups: I (Ginger root), non-viable II and III, and empty egg capsule (IV). The percentage of non-viable capsules (i.e. grades I, II and III) was 92.25%. Empty capsules accounted for 7.75% of the total non-viable egg capsules. Embryonic development was classified into a second scale of eight stages. Egg capsule stage and embryonic stage were significantly related (n = 420; p < 0.001), facilitating the determination of the embryo developmental phase based on the outward appearance of the egg capsules. The embryo development stage based on the external features of the egg capsules might constitute an innovative tool for in situ embryological data collection. This new method is neither time consuming nor invasive, and could be helpful in fishing cruises, for scuba diving visual census in natural habitats and for laboratory culture. Slight variability in the developmental embryonic stages within egg capsules from the same egg mass was identified. The origin of this asynchrony is discussed. Chronological appearance of organs was similar to that of the six loliginid species previously examined. However, some developmental changes in the timing or rate of events (heterochronies) were observed: Hoyle’s organ was formed earlier in L. vulgaris and the appearance of ventral chromatophores was slightly delayed (2 days) compared with the other species considered.     

Reproduction,development and parental care in two direct‐developing flatworms (Platyhelminthes: Polycladida: Acotylea)

Variations in reproductive modes, egg production, and the effects of parental care on hatching success are compared between Pleioplana atomata and Imogine zebra. Pleioplana atomata transfers sperm via true copulation, whereas I. zebra dermally impregnates spermatophores onto the dorsal surface of partners. Pleioplana atomata lays up to 750 large eggs over a 6‐week period, and I. zebra individuals lay up to 1346 small eggs in 12 days. Female fecundity is positively correlated with body size in both species. Developmental time lines are temperature‐dependent, and juvenile worms hatch after 3 and 6 weeks for I. zebra and P. atomata, respectively. Covering of egg masses by the adult is observed for both species and although this parental care is not necessary for egg development or hatching, it plays a significant role in the hatching success of P. atomata. In I. zebra, parental covering of egg masses may play a role in eggshell development.     

Two new Encarsia species (Hymenoptera: Aphelinidae) reared from eggs of Cicadellidae (Hemiptera: Auchenorrhyncha) in Argentina: an unusual new host association

Males of two undescribed Encarsia species were reared from eggs of two cicadellid species during a study of egg parasitoids of corn leafhopper, Dalbulus maidis (DeLong and Wolcott) in northwestern Argentina. Samples were collected during the summer of 2004–2007 using sentinel eggs, and several males of a new species of Encarsia (Hymenoptera: Chalcidoidea: Aphelinidae) were obtained. A single male of a closely related Encarsia species, also previously undescribed, was reared from an egg of another cicadellid on maize, Plesiommata mollicella (Fowler). These are the first records of any Encarsia species reared from eggs of Cicadellidae. The two new species are described as Encarsia dalbulae Polaszek and Luft Albarracin sp. nov., and Encarsia mollicellae Polaszek and Luft Albarracin sp. nov.     

Water temperature at oviposition sites of Rana luteiventris in northeastern Oregon

Water temperature at which Columbia spotted frog ( Rana luteiventris ) eggs were deposited and developed was determined at 18 oviposition sites in northeastern Oregon in 2000. Mean daily water temperature on the day of initial egg deposition ranged from 7.6° C to 16.0° C with a mean of 9.6 ° C. Maximum water temperatures on the 1st day of egg deposition ranged from 9.8° C to 20.2° C and averaged 15.5° C. Eggs were not deposited on days when maximum water temperature was below 9.4° C. Duration of egg deposition ranged from 1 to 20 days at the 18 sites. Embryos hatched after 12 21 days. Mean and maximum daily water temperatures were significantly correlated with number of days to hatching. Key words: Columbia spotted frog, embryonic temperature tolerance, northeastern Oregon, Rana luteiventris, ranid.     

Natural history of the Lined Seedeater Sporophila lineola (Aves: Thraupidae) in southeastern Brazil

The Lined Seedeater (Sporophila lineola) is a migratory species that inhabits a variety of open habitats in South America. We studied the breeding biology and territorial behaviour of a colour-banded population of the species in the Universidade Federal de Viçosa, Campus Florestal (1–19,8808ºS, –44,4136ºW), during two breeding seasons (2014/2015 and 2015/2016), which spans from December to April. We monitored 74 nests of this species. The nest is a low cup supported between a fork. Nests are mainly built with grass stems and rootlets, with spider-web used to hold the material together and to bind the nest to its supporting plant. Females are solely responsible for nest building and incubation, which is synchronic, and also for feeding nestlings to a large extent. Males are responsible for defending the territory, which corresponds to a small portion of the home range restricted to the nest environs, and also feed the nestlings. Mean clutch size is two eggs (78% of nests monitored), with clutches of three (20.3%) and four (1.7%) eggs also observed. Eggs are whitish, covered with dark brown blotches and spots to a variable extent. Mean incubation period, considered as the period between the onset of incubation and hatching of the first egg, is 11 days. Nestling period, considered as the period between hatching of the first egg and fledging of the last young, is 10 days. During the first breeding season, the simple percentage of successful nests was 34.1%, while the Mayfield success was 29.8%, with slightly higher values observed during the second breeding season, with 39.4% and 35.7%, respectively. We recorded, for the first time, three cases of polygamy in the species. We also recorded breeding site fidelity for the first time in the species, with males returning to the same territory owned in a previous breeding season.     

Symbiotic associations between hermit crabs and bryozoans from the Otago region,southeastern New Zealand

Thirteen species of bryozoans (six cyclostomes and seven cheilostomes) occurring off the Otago Peninsula (southeastern New Zealand) form symbiotic, possibly mutualistic, associations with hermit crabs. For all but one of these bryozoan species, such an association has not been reported previously. At most only 3 of the 13 bryozoans are obligate symbionts of hermit crabs. Associations are apparently initiated when a bryozoan larva settles on a gastropod shell occupied by a juvenile hermit crab and develops to form a colony which encrusts the whole shell and then continues to grow out beyond the shell aperture in the form of a helicospiral tube. The tube-building bryozoan colony grows in step with the crab, and tube development appears to be controlled by crab morphology and activity. Of the six species of hermit crabs found occupying bryozoan tubes, four were regular tube occupants, although they could also be found in other shelter types. Hermit crabs occupying bryozoan tubes very rarely indulged in shell exchanges or shell fights. There appear to be no clear cut pairwise correlations between hermit crab species and tube-forming bryozoan species. The following bryozoan species are described for the first time: Heteropora parapelliculata, Borgiola otagoensis, Disporella gordoni, Osthimosia monilifera and O. socialis.     

On systematic relations of Oomorphus concolor (Sturm) Col., Chrysomelidae), with descriptions of its larva and of an aberrant Cryptocephaline larva from Australia

The phylogenetic significance of morphological characters used to diagnose Bactericera, Rhinopsylla, Paratrioza, Allotrioza, Klimaszewskiella, Eubactericera, Phylloplecta and Sinitrioza is examined. There is no evidence that the genera or subgenera, in any of their present or past definitions, constitute natural units. The six former together, however, form a monophyletic group, defined by a combination of adult, larval and egg characters. Phylloplecta and Sinitrioza, which share similar adult characters with Bactericera, differ in the larval and egg morphology. The following new synonymies are established: Bactericera (=Rhinopsylla pro parte, = Paratrioza, = Allotrioza, = Klimaszewskiella, = Eubactericera pro parte) and Phylloplecta (= Sinitrioza). A list of the 121 species included in Bacerticera is provided and 75 new combinations are introduced (60 in Bactericera, 4 in Phylloplecta, 9 in Trioza and 1 in Kuwayama). Bactericera equisetifolii sp. n. and B. vellae sp. n. are described from Israel and Spain respectively, and B. loginovae, B. perrisii and B. rossica are redescribed. The following new species level synonymies are proposed: B. perrisii (= B. maritima) and B. gobica (= P. sinica, = P turcamanica). Variation of genal cone length in B. kratochvili is discussed and the presence of two biological races is noted in B. modesta. Keys are provided for the adults of the European species associated with Salix, and the last instar larvae of the West palaerctic species; the latter are also illustrated. Information on life cycles and host plants is summarised and briefly discussed. Differences in the hindwing venation, which were recently used to erect the families Rhinopsyllidae and Rhinocolidae are shown to be trivial. The two families are therefore synonymised with the Triozidae and Psyllidae respectively.