Release of histamine by cotton dust extracts from human lung tissuein vitro

Zusammenfassung Baumwollextrakt, bei 37° mit zerkleinertem menschlichem Lungengewebe inkubiert, setzt daraus Histamin in ähnlichen Mengen wie Compound 48/80 frei.Die Ergebnisse stützen die Hypothese, dass die Symptome der Byssinosis bei Baumwollspinnerei-Arbeitern mindestens teilweise durch eine Histamin-freisetzende Substanz im Baumwollstaub verursacht werden.     

Release of calcitonin gene-related peptide from a human medullary carcinoma of the thyroid in vitro

Summary The secretion of human calcitonin gene-related peptide was examined in perifusates of medullary carcinoma of the thyroid with a sensitive radioreceptor assay. Calcitonin gene-related peptide was released after the addition of calcium (25–100 mM), in a dose-dependent manner. The results indicate that human medullary carcinomas of the thyroid secrete the calcitonin gene-related peptide as well as calcitonin.     

Release of calcitonin gene-related peptide from a human medullary carcinoma of the thyroid in vitro

The secretion of human calcitonin gene-related peptide was examined in perifusates of medullary carcinoma of the thyroid with a sensitive radioreceptor assay. Calcitonin gene-related peptide was released after the addition of calcium (25-100 mM), in a dose-dependent manner. The results indicate that human medullary carcinomas of the thyroid secrete the calcitonin gene-related peptide as well as calcitonin.     

Release of pre-absorbed cadmium from cultured cells by the addition of copper ion

Summary The biological interaction between cadmium and copper has been studied. Cadmium uptake of KB cells was specifically inhibited by the addition of copper ions to the culture medium. Cadmium which had already been taken up by the cells was released following the addition of copper ions.The authors are grateful to Dr. M. Ishizawa for his helpful discussion.     

Isolation of a protease from the cell-free medium of in vitro cultured mammary carcinoma

Summary Trypsin-like protease with wide spectrum of enzymatic activities have been isolated from cell-free medium from in vitro cultured human mammary carcinoma cells, and from peripheral blood lymphocytes of patients with mammary carcinoma cultured in presence of cell-membrane carcinoma-associated glycoprotein.     

Cultivation in vitro of ciliated epithelium from human nasopharynx

Résumé Des cultures d'épithelium naso-pharyngien ont donné naissance à des couches et des colonnes semblables à celles de l'épithelium in vivo. Cette distribution irrégulière de cellules cilifères, de mucosités, et de cellules polyhédriques ont une action coordonnée qui crée un epithelium auto-détergent qui expulse les corps étrangers.

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This work, under progress, at the University of Singapore is part of the Ph.D. thesis.     

Isolation of a protease from the cell-free medium of in vitro cultured mammary carcinoma.

Trypsin-like protease with wide spectrum of enzymatic activities have been isolated from cell-free medium from in vivo cultured human mammary carcinoma cells, and from peripheral blood lymphocytes of patients with mammary carcinoma cultured in presence of cell-membrane carcinoma-associated glycoprotein.     

Variations in alpha-aminoisobutyric acid transport during in vitro differentiation of cultured myocardial cells from newborn rats]

The transport of alpha-aminoisobutyric acid has been studied as a function of age in heart cell cultures derived from new-born Rats. The specific rate of alpha-aminoisobutyric acid transport decreased slightly once confluency had been reached and then, from day 8 on, increased abruptly. This last phase may be related to the expression of specialized functions encountered in older cultures.     

Chromosome aberrations in in vitro irradiated lymphocytes from human cord blood

Summary An in vitro dose effect curve of dicentric chromosome aberrations in human cord blood lymphocytes has been obtained for 250-kV X-rays. This is compared with a curve prepared in an identical manner using blood from adults. The comparison shows a marginally higher dicentric yield in blood of newborns at doses above about 250 rads.Acknowledgment. We wish to thank Miss P. Orledge of the John Radcliffe Hospital, Oxford, for samples of cord blood, Mr M.J. Corp of the MRC Radiobiology Unit, Harwell, for irradiating the samples and Mr A.A. Edwards of NRPB, for help with statistics. The work was partly supported by Euratom Contract No. 171-76-1 BIO UK.     

Nuclear changes in cultured human dystrophic muscle

Summary Nuclear size in myotubes in cultured human dystrophic muscle has been found to be significantly greater than in normal muscle. These findings, are discussed in relation to the pathogenesis of muscular dystrophy.The authors wish to thank Mrs. L. McGregor and Miss L. Andruzczenko for technical assistance. This work was supported by the Muscular Dystrophy Group of Great Britain. D. V. is in receipt of a scholarship from the State Scholarship Foundation of Greece.     

Heat evolution of cultured human keratinocytes

Summary The heat production of normal and transformed human epidermal keratinocytes precultured in Petriperm ^tm tissue culture dishes was measured calorimetrically. For this purpose, the membrane at the bottom of the culture dish was cut out aseptically and put into a microcalorimeter vessel with the cell layer inwards. A continuous heat output of (83±12) pW/cell was measured for normal keratinocytes from a confluent primary culture. A value of (134±35) pW/cell was obtained when the transformed kerationocyte line SV-K14 was used. The method described in this paper is simple, leads to reproducible results, and can be easily adapted to the calorimetric study of other mammalian cells in vitro.     

Heat evolution of cultured human keratinocytes

The heat production of normal and transformed human epidermal keratinocytes precultured in Petriperm tissue culture dishes was measured calorimetrically. For this purpose, the membrane at the bottom of the culture dish was cut out aseptically and put into a microcalorimeter vessel with the cell layer inwards. A continuous heat output of (83 +/- 12) pW/cell was measured for normal keratinocytes from a confluent primary culture. A value of (134 +/- 35) pW/cell was obtained when the transformed keratinocyte line SV-K14 was used. The method described in this paper is simple, leads to reproducible results, and can be easily adapted to the calorimetric study of other mammalian cells in vitro.