L'évolution de la symbiose chez les Isoptères

Summary An attempt is made to retrace the evolution of symbiotic associations in Blattopteroidea, with particular reference to Isoptera. It seems probable that double symbiosis occured in the common ancestor of Blattids and Termites: intracellular microorganisms of bacteriocytes, xylophagous intestinal Flagellates. Where as actual Blattids, exceptingCryptocercus, have only retained the former, the primitive Termites, exceptingMastotermes, have only kept the latter. Evolved Termites (fam. Termitidae) have in turn lost the symbiotic Flagellates, but have acquired a rich and complex bacterial flora. — Some of these microorganisms, mixed with their aliments, certainly aid digestion (breakdown of cellulose), whilst others, localised at a particular level of the alimentary canal and not found in the alimentary mass, presumably play a different rôle. Furthermore, Protozoa are constantly associated with certains Termites (xylophagous Amoebae, humus-feeder Ciliates of the genusTermitophrya) and may play some rôle in the process of digestion. Finally, an association occurs between fundgus-growing Termites (subfam. Macrotermitinae) and mushrooms (Termitomyces).     

The C4-dicarboxylate transport system ofRhizobium meliloti and its role in nitrogen fixation during symbiosis with alfalfa (Medicago sativa)

TheRhizobium meliloti C₄-dicarboxylate transport (Dct) system is essential for an effective symbiosis with alfalfa plants. C₄-dicarboxylates are the major carbon source taken up by bacteroids. Genetic analysis of Dct^– mutant strains led to the isolation of thedct carrier genedctA and the regulatory genesdctB anddctD. The carrier genedctA is regulated in free-living cells by the alternative sigma factor RpoN and the two-component regulatory system DctB/D. In addition, DctA is involved in its own regulation, possibly by interacting with DctB. In bacteroids, besides the DctB/DctD system an additional symbiotic activator is thought to be involved indctA expression. Further regulation ofdctA in the free-living state is reflected by diauxic growth of rhizobia, with succinate being the preferred carbon source. The tight coupling of C₄-dicarboxylate transport and nitrogen fixation is revealed by a reduced level of C₄-dicarboxylate transport in nitrogenase negative bacteroids.     

Evidence that two species of aphid ingest food through an open stylet sheath

Zusammenfassung Myzus persicae undAcyrthosiphon pisum nehmen beim Saugen an Saccharoselösungen Kohlenstoffteilchen bis zu einem Durchmesser von 0,5–1 auf (ungefähre Weite des Nahrungskanals der Stechborsten). Demnach muss die um die Stechborsten ausgebildete Speichelscheide am Ende offen sein. BeiA. pisum konnte beobachtet werden, dass grössere Kohlenstoffteilchen an der Stechborstenspitze das Saugen deutlich behindern, was durch Bewegungen der Stechborsten und erneute Speichelabgabe behoben werden konnte.

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The contribution of this author was made while on sabbatical leave from the Waite Institute, University of Adelaide (South Australia), and supported in part by Public Health Service Training Grant No. 5T1-AI-218, from the National Institute of Allergy and Infectious Diseases (USA).     

Function and evolution of nodulation genes in legumes

Root nodule (RN) symbiosis has a unique feature in which symbiotic bacteria fix atmospheric nitrogen. The symbiosis is established with a limited species of land plants, including legumes. How RN symbiosis evolved is still a mystery, but recent findings on legumes genes that are necessary for RN symbiosis may give us a clue.     

Chronic mercury vapor poisoning of aphids

Summary Exposure of green peach aphids,Myzus persicae (Sulz.), to an atmosphere containing mercury vapor resulted in a curtailment ofembryogenesis and larviposition by adults, and in the development by larvae and adults of a cuticular darkening of their legs, head capsule, antennae, cornicles and cauda. Mortality of affected larvae resulted from molting difficulties, particularly by last-instar alatiform female and male larvae. Greenbugs,Schizaphis graminum (Rond.), and pea aphids,Acyrthosiphon pisum (Harr.), responded to mercury vapor exposure in similar ways.     

A peculiar fatty acid, (Z,Z)-9,12,17-octadecatrienoic acid,identified in the phospholipids of the pea aphid,Acyrthosiphon pisum (Harris) (homoptera: aphididae)

A peculiar fatty acid previously detected in the phospholipids of the pea aphid,Acyrthosiphon pisum, is identified as (Z,Z)-9,12,17-octadecatrienoic acid. It is the first report of this compound in the literature. Comparison of fatty acid profiles of phospholipids between normal and aposymbiotic pea aphids shows that aphid symbionts are not responsible for the biosynthesis of this unusual fatty acid.     

Morphological,biochemical and molecular changes during ectomycorrhiza development

Summary An ectomycorrhiza, a specialized root organ, is the result of a complex interaction leading to a finely-tuned symbiosis between a plant and a compatible ectomycorrhizal fungus. Ultrastructural observations combined with cytochemical and biochemical studies reveal that structural and metabolic changes in the symbiont cells lead to the final phenotype of the active ectomycorrhiza. In the present review these changes are interpreted as changes in gene expression and discussed within the context of ectomycorrhiza development. Recent genetic data indicate that the continued vegetative growth of the ectomycorrhizal hyphae and the root tissues, and their ability to switch to symbiotic organ formation, is basically controlled by developmentally critical genes. The activity of these symbiotic genes during the differentiation of ectomycorrhizas is associated with extensive changes in the concentration of particular polypeptides and protein biosynthesis. The present state of knowledge about the developmental biology of ectomycorrhizas allows only speculation about the events during their development.Puisant mes forces aux sources des galaxies En buvant la sève des arbres M. Jonasz     

Two classes of metabolites fromTheonella swinhoei are localized in distinct populations of bacterial symbionts

The marine spongeTheonella swinhoei (lithistid Family Theonellidae, Order Astrophorida) has yielded many important, bioactive natural products, most of which share structural features with bacterial natural products. The presence of microbial symbionts inT. swinhoei has been reported, and it was originally suggested that the cytotoxic macrolide swinholide A and many of the bioactive cyclic peptides fromT. swinhoei were all produced by symbiotic cyanobacteria. By transmission electron microscopy, we found four distinct cell populations to be consistently present inT. swinhoei: eukaryotic sponge cells, unicellular heterotrophic bacteria, unicellular cyanobacteria and filamentous heterotrophic bacteria. Purification and chemical analyses of each cell type showed the macrolide swinholide A to be limited to the mixed population of unicellular heterotrophic bacteria, and an anti-fungal cyclic peptide occurred only in the filamentous heterotrophic bacteria. Contrary to prior speculation, no major metabolites were located in the cyanobacteria or sponge cells.     

Cellulase genes from the parabasalian symbiont <Emphasis Type="Italic">Pseudotrichonympha grassii</Emphasis> in the hindgut of the wood-feeding termite <Emphasis Type="Italic">Coptotermes formosanus</Emphasis>

Cellulase genes of Pseudotrichonympha grassii (Hypermastigida: Eucomonymphidae), the symbiotic flagellate in the hindgut of the wood-feeding termite Coptotermes formosanus, were isolated and characterized. The nucleotide sequences of the major cellulase component in the hindgut of C. formosanus were determined based on its N-terminal amino acid sequence. The five isolated nucleotide sequences (PgCBH-homos) had an open reading frame of 1350 bp showing similarity to catalytic domains of glycoside hydrolase family (GHF) 7 members, and primary structure comparison with GHF7 members whose tertiary structures are well-characterized revealed the overall similarity between PgCBH-homo and the catalytic domain of a processive cellulase Cel7A (formerly CBHI) from the aerobic fungus Trichoderma reesei. Functional expression of PgCBH-homos in Escherichia coli, using the carboxymethylcellulose-Congo red assay, demonstrated the actual cellulolytic activity of PgCBH-homo. RT-PCR showed that PgCBH-homos were expressed, from the three flagellates in the hindgut, specifically in P. grassii. Received 10 July 2002; accepted 26 July 2002 RID="*" ID="*"Corresponding author.     

Infection of frog tadpoles (Amphibia) by insect parasitic nematodes (Rhabditida)

Summary Infective stage juveniles ofNeoaplectana carpocapsae (Steinernematidae) andHeterohabditis heliothidis (Heterorhabditidae) were able to penetrate through the alimentary tract of young tadpoles ofHyla regilla (Hylidae) andXenopus laevis (Pipidae) and enter the body cavity. Some infectives ofN. carpocapsae were able to release their symbiotic bacterium,Xenorhabdus nematophilus inside the host and in two cases, the nematodes developed into adult females before they perished. Tadpole mortality was associated with foreign bacteria entering the penetration holes made by the invading nematodes. The infective stage juveniles of both nematodes frequently encountered a host defense reaction upon reaching the tadpole's infective stage juveniles of both nematodes frequently encountered a host defense reaction upon reaching the tadpole's coelom.     

Using artificial systems to explore the ecology and evolution of symbioses

The web of life is weaved from diverse symbiotic interactions between species. Symbioses vary from antagonistic interactions such as competition and predation to beneficial interactions such as mutualism. What are the bases for the origin and persistence of symbiosis? What affects the ecology and evolution of symbioses? How do symbiotic interactions generate ecological patterns? How do symbiotic partners evolve and coevolve? Many of these questions are difficult to address in natural systems. Artificial systems, from abstract to living, have been constructed to capture essential features of natural symbioses and to address these key questions. With reduced complexity and increased controllability, artificial systems can serve as useful models for natural systems. We review how artificial systems have contributed to our understanding of symbioses.     

Conversion of phenylalanine to toluene and 2-phenylethanol by the pine engraverIps pini (Say) (Coleoptera,Scolytidae)

Summary The pine engraver,Ips pini (Say), was found to produce toluene and 2-phenylethanol when boring into fresh pine logs. The hypotheses that phenylalanine is a precursor of these compounds and that beetles without their symbiotic microorganisms can perform these conversions were confirmed by treating wild and axencially-reared males and females topically with L-phenyl-d₅-alanine. Extracts of these beetles invariably contained deuterio-toluene, and extracts of males contained deuterio-2-phenylethanol as well.     

The Spectacle de la Nature in Eighteenth-Century Spain: From French Households to Spanish Workshops

This paper analyzes the Spanish appropriation of one of the great French eighteenth-century best-sellers, the Spectacle de la Nature (1732--1750) by the abbé Antoine Nöel Pluche. In eight volumes, the abbé discussed current issues in natural philosophy, such as Newtonianism, the origin of fossils, artisan techniques, natural history, machines, gardening or insect-collection in a polite-conversation format. It was translated into English (1735), Dutch (1737), Italian (1737), German (1746) and Spanish (1753). But the four Spanish editions were very different from their European counterparts. In Spain, it was delivered in 16 carefully printed and extensively commented volumes. In Pluche's original, there was a concern for the young gentleman's education, new pedagogical methods and an enthusiastic defence of experimental knowledge. However, Le Spectacle in Spain was conceived as a useful tool for modernizing the country, it served political and propagandist goals, defended Spanish culture and science (in particular with respect to American flora, fauna and geography) and the Jesuit contribution to science and aimed to harmonize experimental knowledge and scholastic tradition. The analysis of the more than 1500 footnotes, prefaces, some readers’ comments and other questions related to the format gives insight on how it was appropriated.     

Competitiveness and communication for effective inoculation byRhizobium,Bradyrhizobium and vesicular-arbuscular mycorrhiza fungi

After a short summary on the ecology and rhizosphere biology of symbiotic bacteria and vesicular-arbuscular (VA) mycorrhiza fungi and their application as microbial inocula, results on competitiveness and communication are summarized. Stress factors such as high temperature, low soil pH, aluminium concentrations and phytoalexins produced by the host plants were studied withRhizobium leguminosarum bv.phaseoli andRhizobium tropici onPhaseolus beans. Quantitative data for competitiveness were obtained by usinggus ⁺ (glucoronidase) labelled strains, which produce blue-coloured nodules. ForPhaseolus-nodulating rhizobia, a group specific DNA probe was also developed, which did not hybridize with more than 20 other common soil and rhizosphere bacteria. Results from several laboratories contributing to knowledge of signal exchange and communication in theRhizobium/Bradyrhizobium legume system are summarized in a new scheme, including also defense reactions at the early stages of legume nodule initiation. Stimulating effects of flavonoids on germination and growth of VA mycorrhiza fungi were also found. A constitutive antifungal compound in pea roots, -isoxazolinonyl-alanine, was characterized.     

Biochemistry of frog ribonucleases

Frogs have unique pyrimidine base-specific RNases, with structures similar to those of turtle, iguana and chicken RNases. Among the four frog RNases discussed here, three from Rana pipiens, R. catesbeiana and R. japonica oocyte cells show anti-tumour activity, and the latter two show lectin activity towards sialic acid-rich glycoproteins. In this review, (i) we compare their unique primary structures with respect to the locations of their disulphide bridges, three-dimensional structure, base specificity and heat stability as compared with RNase A, and (ii) we summarize current knowledge about the mode of action of lectin and the antitumour activities of the three frog RNases.     

Interaction ofRhizobium loti strain and host on the biosynthesis of unusual amino acids in leguminous plants

Summary The effect of differentRhizobium loti strains on the biosynthesis of 2,3-diamino-butanoic acid and 2,4-diamino-3-methyl-butanoic acid in root nodules ofLotus tenuis, Anthyllis vulneraria andLupinus densiflorus has been investigated. Results suggest that biosynthesis isRhizobium strain dependent, that the bacteroid is the site of synthesis of the compounds and that their biosynthesis is confined to the symbiosis.     

Aseptic rearing of the aster leafhopper,Macrosteles fascifrons (Stål), on a chemically defined diet

Summary The normal symbiotic aster leafhoppers,Macrosteles fascifrons, were reared under aseptic conditions on the holidic diet through 5 successive generations with normal rates of development, survival and reproduction. Thus the artificial diet is completely adequate for normal insects and dependence on gut organisms is not part of the physiology of this species.     

Multi‐step forecasting for long‐memory processes

In this paper we present results of a simulation study to assess and compare the accuracy of forecasting techniques for long‐memory processes in small sample sizes. We analyse differences between adaptive ARMA(1,1) L‐step forecasts, where the parameters are estimated by minimizing the sum of squares of L‐step forecast errors, and forecasts obtained by using long‐memory models. We compare widths of the forecast intervals for both methods, and discuss some computational issues associated with the ARMA(1,1) method. Our results illustrate the importance and usefulness of long‐memory models for multi‐step forecasting. Copyright © 1999 John Wiley & Sons, Ltd.     

A new parsimonious recurrent forecasting model in singular spectrum analysis

Singular spectrum analysis (SSA) is a powerful nonparametric method in the area of time series analysis that has shown its capability in different applications areas. SSA depends on two main choices: the window length L and the number of eigentriples used for grouping r. One of the most important issues when analyzing time series is the forecast of new observations. When using SSA for time series forecasting there are several alternative algorithms, the most widely used being the recurrent forecasting model, which assumes that a given observation can be written as a linear combination of the L?1 previous observations. However, when the window length L is large, the forecasting model is unlikely to be parsimonious. In this paper we propose a new parsimonious recurrent forecasting model that uses an optimal m(<L?1) coefficients in the linear combination of the recurrent SSA. Our results support the idea of using this new parsimonious recurrent forecasting model instead of the standard recurrent SSA forecasting model.