聚乙二醇-丝素共混膜物理性能的研究

不同相对分子质量的聚乙二醇(PEG)以不同比例混入丝素可形成共混丝素膜，并具有良好的拉伸性、透水、透；气、透光性和一定的生物降解与溶解性。实验结果表明：虽PEG和丝素的可混合性稍差，但在一定的透水、透汽性和较好的柔韧性前提下，可达到创面膜的基本要求。     

壳聚糖—明胶共混膜

用溶液共混法成功地制备了壳聚糖与明胶共混膜,并用红外光谱,Ｘ射线衍射,扫描电镜,透光率,吸水率及力学性能测试对共混膜进行了表征。结果表明,共混膜中壳聚糖分子与明胶分子间存在强的相互作用及良好扔相容性,壳聚糖的引入有利于减小明胶的吸水率,改善其力学性能。     

离子液体中丝素/聚乙烯醇共混膜的制备及表征

采用离子液体氯化1-丁基-3-甲基咪唑（BMIMCl）溶解丝素（SF）,获得BMIMCl/SF溶液,再与聚乙烯醇（PVA）水溶液共混,制备得到PVA/SF共混膜。共混膜经红外光谱、紫外可见光谱、对水接触角、拉力试验等表征。结果表明,溶液共混使共混膜中SF与PVA发生了化学键键合,乙醇处理使共混膜中SF主要以β-折叠结构存在。随着SF含量的增加,共混膜的透光率逐渐变小,亲水性则逐渐增大。     

模板法制备明胶多孔微球

以单分散聚苯乙烯(PS)微粒为致孔剂,采用模板法制备了一种新型的明胶多孔微球,并用扫描电子显微镜(SEM)对其进行了表征.结果表明,通过调节PS微粒的用量和粒径大小可以控制微球和孔径的大小,且微球表面有均匀的孔结构.     

明胶分离膜的制备

首次报道明胶单独用作分离膜材料,对所选用明胶的粘度、粘度下降值、含水量、pH值、冻点等性能指标全面表征,铸膜液组成及铸膜工艺条件是影响分离膜性能的重要因素,通过实验选择了合适的铸膜液明胶浓度、温度和pH值,并对适量Cr_2(SO_4)_3添加到铸膜液可使明胶膜耐水性、强度及抗生物降解性明显提高的原因进行深入分析。     

肝素化丝素／聚氨酯共混膜的制备及其缓释性能

以自制的非水溶性丝素粉体为载体,将肝素与医用聚氨酯同溶液混合,制备出含有肝素的丝素/聚氨酯共混膜,解决了肝素与聚氨酯同溶液共混成膜时团聚的问题,并利用丝素蛋白良好的吸附和缓释功能,使肝素从共混膜中缓慢释放出来,达到相对长效的抗凝血效果.利用ATR-FTIR分析了肝素加入后丝素粉体、丝素/聚氨酯共混膜的红外光谱的变化,并对共混膜中肝素的缓释性能及共混膜在PBs溶液中浸泡48h前后的形貌变化进行了研究.     

多孔丝素材料的结构与性能研究

用CaCl2.CH3CH2OH.H2O三元溶剂溶解蚕丝丝素,通过对丝素溶液冷冻之后的状态,以及冷冻干燥后丝素结构和性质的测试和分析,指出采用冷冻干燥制备再生蚕丝丝素多孔材料时,若冷冻温度低于－20℃,则丝素的结构以无定型为主,含少量的SilkII,若冷冻温度高于－20℃,则又增加了较多的SilkI,采用冷冻干燥法可以制得平均孔径为10－300um,孔密度为1－2000个／mm^2,孔隙率为35％－70％的多孔丝素材料,通过调节冷冻温度和丝素溶液质量分数,达到控制多孔丝素材料的上述表态结构,聚集态结构和透湿性,压缩性,强度,伸长率等物理性能的目的是可能的,将快速冷冻的丝素水溶液真空干燥之前,进行反复解冻－冷冻－则所制得的多孔丝素材料的孔径增大,孔密度减小,同时其压缩率和透湿性有所提高,拉伸断裂强度和在热水中的溶失率下降。     

多孔阳极氧化铝膜制备方法的研究进展

多孔阳极氧化铝膜(Anodic Aluminum oxide,AAO)具有孔洞垂直于铝基底且平行排列的六方连续结构,因其孔间距高度可调,孔径分布均匀,在催化、合成金属纳米线、传感、过滤和仿生等领域都有很高的应用价值。简要介绍了自组织有序多孔AAO膜的结构参数特点,并简述了制备自组织AAO模板不同制备方法的研究进展,力求思路清晰并合乎逻辑。     

以TiAl金属间化合物为支撑体的多孔Ni膜的制备

为了克服传统有机膜与陶瓷膜的一些不足之处,以TiAl金属间化合物为支撑体,用悬浮粒子烧结法在孔径为10．6um的片状支撑体上制备多孔金属Ni膜。考察了浸浆时间、烧结温度等工艺参数对膜表面形貌和孔径大小、分布等膜性能的影响。结果表明,合适的制膜条件是：浸浆时间为60s,烧结温度为500℃。得到了孔径分布比较窄而且表面比较平整的微滤膜,其平均孔径为0．83μm,膜厚约为30μm。     

多孔氧化铝膜的制备

采用阳极氧化法在硫酸、草酸、磷酸等电解液中,在高纯度(99.9%)铝片上制备多孔氧化铝膜.结果表明:电解液的类型将影响氧化铝膜上孔洞的孔径大小;而电解液浓度的大小(硫酸取0.38 M～3.80 M,磷酸取1.75 M～5.20 M,草酸取0.11 M～0.56 M)、氧化电压的高低(硫酸电解液电压10～30 V,磷酸电解液电压30～80 V,草酸电解液电压20～100 V)和氧化时间的长短都会影响氧化铝孔膜的形态.     

蚕丝蛋白/明胶多孔肝组织支架的制备及性能研究

采用冷冻干燥法制备了蚕丝蛋白/明胶多孔支架,并分别在-20℃和-70℃预冻,以观察支架的微孔结构,测量支架的孔隙率、溶胀吸水性及力学性能,研究HepG2细胞在支架中的生长增殖情况.结果表明:随预冻温度降低,支架的孔径减小且微孔分布更加均匀,孔隙率及力学性能均得到提高.细胞培养结果显示:随预冻温度降低,支架的细胞贴壁率减...     

Structure and Property of Silk Fibroin ／ Cellulose Blend Film

Silk fibroin/cellulose blend films were prepared using Nmethylmorpholine-N-oxide (NMMO) as solvent. The effects of different proportions and solid contents on properties of blend films were discussed. The mechanical properties showed that the blend films had preferable moisture permeability and a high strength. The structures of the blend films were investigated by infrared spectrum and X-ray diffraction. The results indicated the occurrence of hydrogen bonds between hydroxyl groups of cellulose and amido groups of fibroin.     

天然丝素制备三维多孔支架

研究盐沥滤法制备三维多孔丝素支架,在丝素多孔支架上培养人成骨肉瘤细胞、成纤维细胞及肝细胞.研究材料的细胞相容性,发现多种动物细胞在盐沥滤法所制备的丝素多孔支架材料上能够很好地黏附和增殖.通过调整丝素水溶液的浓度、溶解丝素溶剂体系、NaCl添加量和NaCl粒径,制备出结构和性质可以调控的三维支架.该结果为丝素多孔支架材料的制备提供了新的研究思路和方法.     

Structure and Properties of Porous Silk Fibroin Materials

In this article the structure and properties of freeze-dried porous silk fibroin materials were tested and analyzed.The results indicated that for porous silk fibroin materials prepared by means of freeze-drying,if freez-ing temperature was below —20℃,the structure of silk fibroin was mainly amorphous with a little silk Ⅱ crystal structure,and if freezing temperature was above —20℃,quite a lot of silk Ⅰ crystal structure formed.Porous silk fibroin materials,with average pore diameter be-     

Physico-Chemical Properties of a Water-Insoluble Silk Fibroin Film

The conventional silk fibroin(SF)films obtained by casting from SF aqueous solution are amorphous,and easily dissolved in water.This defect limits the practical use of SF films in biomedical field.A new water-insoluble silk fibroin film(an SF-FA film)could be simply prepared by casting from SF formic acid solution.The physical properties and moisture permeability of SF-FA films were examined.The results show that the SF-FA film swollen in water exhibits outstanding pliability.The weight loss of SF-FA films ...     

明胶膜的制备及性能

用浸涂的方法制备了明胶膜,测定了膜的机械性能、溶胀性能、对水的敏感性以及膜的含水量,并探讨了涂布温度、成膜液的pH、增塑剂甘油的添加量、交联剂的种类及添加量以及贮存环境湿度对明胶膜性能的影响。研究结果表明:用浸涂方法制备明胶膜时,w=0.12的明胶成膜液适宜的涂布温度为33~38℃;成膜液的pH在7左右时,膜的抗拉强度最大;随着增塑剂甘油用量增加,膜的抗拉强度下降,而断裂伸长率增大;交联剂甲醛和明矾都有明显的交联作用,但交联剂的添加量过多时,不适合用浸涂的方法制备膜;随着贮存湿度的增加,膜的抗拉强度和弹性模量逐渐降低,断裂伸长率和含水量呈递增趋势。     

Preparation of Nano Silver/Silk Fibroin Composite Films

In this study, the outstanding biocompatibility of silk fibroin （SF） and the highly efficient anti-bacterial effect of nano silver （NS） were utilized to prepare SF/NS composite film with anti- bacterial property. The structure and property of the film were characterized. The results showed that the structure of SF in the film was mainly silk I. SF in the film was almost insoluble in water. The tensile strength of film with NS was significantly lower than that of films without NS. When the addition of NS was within the range of 0%-0.6%, the elongation at break had no significant difference. The antibacterial rate of the film on staphylococcus aurens and escherichia coil increased with the amount of NS. The minimum amount of NS in the fdm was O. 1% and the maximum amount was 0.5%.     

Tussah Silk Fibroin Porous Scaffolds Prepared with a Mild Self-assembly Process for Controlled Drug Release

Besides excellent biodegradability and biocompatibility,a useful tissue engineering scaffold should provide favorable surface properties,outstanding mechanical strength and controlled drug release property. In this paper,a mild process to prepare porous tussah silk fibroin（ TSF） scaffolds from aqueous solution was described. The n-butanol was used to control the self-assembly of tussah silk. The scaffolds with different TSF concentrations and the same volume showed differences in pore size and distribution. The maximum porosity of the poprepared porous scaffolds was 80% in this paper. And the pore size of the prepared porous scaffolds with different concentrations was between 10μm and 230 μm. X-ray diffraction（ XRD） analysis revealed that amorphous TSF was crystallized to β-sheet secondary structure upon gelatin. The TSF scaffolds for controlled drug release was studied and the result showed that the time of drug release was significantly longer. The produced TSF scaffolds with sustained drug release have potential application in tissue engineering.     

Silk Fibroin Film Blended with Poly（ ethylene glycol-glycerin）

To improve the toughness of silk fibroin（ SF） films,poly（ ethylene glycol-glycerin）（ PEGG） was synthesized with ethylene glycol and epichlorohydrin. The SF / PEGG blend films were prepared by casting aqueous solution and their structures were characterized. The PEGG was in liquid state at room temperature so it will not be a single phrase at blend film. It crosslinked with SF and made it insolubility in water. The results of X-ray diffraction（ XRD） indicated that the crystallinity of the SF in the blend films decreased with the content of PEGG increasing. The tensile strength and elongation at break of blend films were measured using an instron tensile tester. The results showed that the tensile strength and elongation at break of blend films were high enough for application.After the blend films were stored at room temperature for 100 d,the crystallinity, the tensile strength and elongation at wet state increased. The blend films are superior to SF films in providing excellent flexibility and mechanical properties in both dry and wet states. Based on the fact that SF has good biocompatibility,the SF /PEGG blend film will offer new options in many different biomedical applications.