Sympathectomy enhances the substance P-mediated breakdown of the blood-aqueous barrier in response to infrared irradiation of the rabbit iris

Summary Rabbits were subjected to infrared irradiation of the iris 1 month after unilateral cervical sympathectomy. The resulting breakdown of the blood-aqueous barrier was greatly enhanced on the sympathectomized side. In contrast, the response to intravitreally injected substance P (SP) was the same in both eyes. The enhancement of the response to IR irradiation could be abolished by pretreatment with an SP antagonist, (D-Pro², D-Trp^7,9)-SP.This study was supported by grants from the Swedish MRC (04X-1007, 14X-2321), the Medical Faculty of Lund, Ferring Arzneimitel, Kiel (Federal Republic of Germany) and Ferring Pharmaceuticals, Malmö (Sweden).     

A MSH-released serum factor acting on the blood-aqueous barrier of the rabbit eye

Résumé L'injection sous-cutanée de l'hormone synthétique-MSH en dose de 10g/kg peut produire chez le lapin une augmentation de la perméabilité dans l'il. Le sérum qui a été prélevé à ces lapins contient un facteur non-dialysable et de grandes molécules. Injecté par voie intraveineuse à d'autres lapins, ce facteur augmente la perméabilité oculaire.     

Biochemical evidence for two types of noradrenaline storage particles in rabbit iris

Summary Centrifugation techniques were used to determine the subcellular distribution of noradrenaline (NA) and dopamine -hydroxylase (DH) in the rabbit iris. By application of isopycnic and differential gradient centrifugation methods 2 types of NA vesicles could be demonstrated. Of the total particle bound NA about 70% is associated with light and about 30% with heavy vesicles. For both types of vesicles the distribution of DH reflected that of NA.     

Transport of the synthetic peptide DSIP through the blood-brain barrier in rabbit

Summary The synthetic delta sleep inducing peptide (DSIP) passes the blood-brain barrier, since i.v. injection in free moving rabbits (30 nmoles/kg) significantly increases the cortical delta activity and decreases the motor activity during 5 h.Acknowledgment. This work was supported by grants from the Swiss National Science Foundation (Nos 3.871-72, 3.525.75, 3.443.074, 3.780.076), Fonds für Lehre und Forschung der Universität Basel, Merck Sharp and Dohme (USA), Ciba-Stiftung Basel.     

Transport of the synthetic peptide DSIP through the blood-brain barrier in rabbit

The synthetic delta sleep inducing peptide (DSIP) passes the blood-brain barrier, since i.v. injection in free moving rabbits (30 nmoles/kg) significantly increases the cortical delta activity and decreases the motor activity during 5 h.     

The effect of pyridoxine and pyridoxal on the circulatory response of rats to microwave irradiation

Résumé L'administration intraveineuse de pyridoxine et pyridoxal (10 mg/kg) à des rats anesthésiés inhibe l'augmentation du rendement du cocur. En même temps, une hyperthermie est provoquée par l'irradiation du corps entier avec des ondes de longueur minime (2,450 Mc. c.w. 0.08/w/cm²). Cet effet peut être attribué à l'interaction de ces composés d'amines vasoactifs libérés pendant l'irradiation.     

A simple technique for testing the in vitro response of rabbit lymphocytes to PHA and allogeneic cells

Summary Lymphocytes of rabbits can be separated from small quantities of heparinized whole blood using a simple density gradient of Ficoll-Ronpacon 1.09. This separation technique yields a pure suspension of viable cells allowing reproducible results from cultures stimulated either with PHA or allogeneic lymphocytes isolated by the same technique.Supported by the Swiss Science Foundation No. 3.890-0.77.     

A simple technique for testing the in vitro response of rabbit lymphocytes to PHA and allogeneic cells.

Lymphocytes of rabbits can be separated from small quantities of heparinized whole blood using a simple density gradient of Ficoll-Ronpacon 1.09. This separation technique yields a pure suspension of viable cells allowing reproducible results from cultures stimulated either with PHA or allogeneic lymphocytes isolated by the same technique.     

Bayliss myogenic response in the isolated ductus arteriosus of guinea-pig and rabbit fetuses

Summary Isolated ductus arteriosus responds by marked constriction to increases in perfusion pressure. If, however, these increases exceed 90 mm Hg-130 mm Hg, the vessel suddenly dilates and its responsiveness becomes depressed. The importance of these findings in postnatal closure of ductus arteriosus is discussed.     

The effect of some anticholinesterase agents and of hemicholinium on the amount of substance P in rabbit brain and gut

Résumé Les substances anticholinestérasiques s'avèrent capables de réduire la concentration en substance P du cerveau et de l'intestin du lapin. Cependant l'hémicholinium No 3 augmente la concentration de la substance P de l'intestin et diminue celle du cerveau.     

Podocalyxin enhances the adherence of cells to platelets

Podocalyxin (PODXL) is a mucin protein of the CD34 family expressed in kidney glomerular podocytes, vascular endothelium, progenitor bone marrow and tumor cells. It is assumed that PODXL plays an anti-adherent role in kidney podocytes. CHO cells stably expressing human PODXL (CHO-PODXL) or human tumor cells (Tera-1) inherently expressing PODXL showed increased adherence to platelets. The adherence of cells was inhibited (70%) by blockers of platelet P-selectin, prevented by the soluble ectodomain of human PODXL (PODXL-Δ) or by the arginine-glycine-aspartate (RGDS) peptide and partially impeded by inhibition of integrin αVβ3/αVβ5, suggesting a coordinated action of P-selectin and integrins. Colocalization of platelet P-selectin and PODXL expressed on CHO cells was demonstrated by confocal immunofluorescence. No adherence to platelets was observed when PODXL was expressed in glycomutant CHO cells deficient in sialic acid. Received 14 August 2007; received after revision 12 September 2007; accepted 13 September 2007