共查询到20条相似文献,搜索用时 15 毫秒
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Base sequence discrimination by zinc-finger DNA-binding domains 总被引:49,自引:0,他引:49
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M G Peterson J Inostroza M E Maxon O Flores A Admon D Reinberg R Tjian 《Nature》1991,354(6352):369-373
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DNA sequence and expression of the B95-8 Epstein-Barr virus genome 总被引:34,自引:0,他引:34
R Baer A T Bankier M D Biggin P L Deininger P J Farrell T J Gibson G Hatfull G S Hudson S C Satchwell C Séguin 《Nature》1984,310(5974):207-211
The complete (172,282 base pairs) nucleotide sequence of the B95-8 strain of Epstein-Barr virus has been established using the dideoxynucleotide/M13 sequencing procedure. Many RNA polymerase II promoters have been mapped and the mRNAs from these promoters have been assigned to the latent or early/late productive virus cycles. Likely protein-coding regions have been identified and three of these have been shown to encode a ribonucleotide reductase, a DNA polymerase and two surface glycoproteins. 相似文献
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Ineukaryotes,alargenumberofsmallnucleolar RNAs(snoRNAs)accumulatedwithinthenucleolus playimportantrolesinprecursorribosomalRNA(pre RNA)processingandmaturation[1].AllsnoR NAs,withtheexceptionofRNaseMRP,canbe broadlydividedintotwoexpendinggroups,boxC/D andH/ACAsnoRNAs,basedonconservedsequence elements[2].BoxC/DsnoRNAscontaintwocon servedshortsequencemotifs,boxC(UGAUGA)and boxD(CUGA),locatedonlyafewnucleotidesaway fromthe5′and3′ends,respectively,generallyas partofatypical5′3… 相似文献
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CHEN Yancheng YANG Ence LIU Zhenfeng TIAN Zhexian WANG Yiping 《科学通报(英文版)》2006,51(8):934-940
In prokaryotic cells, although the σ54 RNA poly- merase can stably bind to the σ54-dependent promoter without the enhancer-binding proteins (EBPs), it re- mains as a closed complex which is silent for transcrip- tion[1]. When binding to the enhancer-lik… 相似文献
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Frazão C McVey CE Amblar M Barbas A Vonrhein C Arraiano CM Carrondo MA 《Nature》2006,443(7107):110-114
RNA degradation is a determining factor in the control of gene expression. The maturation, turnover and quality control of RNA is performed by many different classes of ribonucleases. Ribonuclease II (RNase II) is a major exoribonuclease that intervenes in all of these fundamental processes; it can act independently or as a component of the exosome, an essential RNA-degrading multiprotein complex. RNase II-like enzymes are found in all three kingdoms of life, but there are no structural data for any of the proteins of this family. Here we report the X-ray crystallographic structures of both the ligand-free (at 2.44 A resolution) and RNA-bound (at 2.74 A resolution) forms of Escherichia coli RNase II. In contrast to sequence predictions, the structures show that RNase II is organized into four domains: two cold-shock domains, one RNB catalytic domain, which has an unprecedented alphabeta-fold, and one S1 domain. The enzyme establishes contacts with RNA in two distinct regions, the 'anchor' and the 'catalytic' regions, which act synergistically to provide catalysis. The active site is buried within the RNB catalytic domain, in a pocket formed by four conserved sequence motifs. The structure shows that the catalytic pocket is only accessible to single-stranded RNA, and explains the specificity for RNA versus DNA cleavage. It also explains the dynamic mechanism of RNA degradation by providing the structural basis for RNA translocation and enzyme processivity. We propose a reaction mechanism for exonucleolytic RNA degradation involving key conserved residues. Our three-dimensional model corroborates all existing biochemical data for RNase II, and elucidates the general basis for RNA degradation. Moreover, it reveals important structural features that can be extrapolated to other members of this family. 相似文献
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Specific binding of the transcription factor sigma-54 to promoter DNA. 总被引:11,自引:0,他引:11
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RNA polymerase II elongation through chromatin 总被引:21,自引:0,他引:21
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Transient cyclical methylation of promoter DNA 总被引:3,自引:0,他引:3
Kangaspeska S Stride B Métivier R Polycarpou-Schwarz M Ibberson D Carmouche RP Benes V Gannon F Reid G 《Nature》2008,452(7183):112-115