Bt毒蛋白基因重组大肠杆菌的杀虫活性研究

本文对Bt毒蛋白基因(cry1Aa)重组大肠杆菌(ECE52)的发酵条件及杀虫活性进行了研究,结果表明该菌在37℃下培养或发酵8～12h较为合适.经超声波破碎的菌体对家蚕4龄幼虫有较强的毒性,用62.5×10-6g/mL的菌体稀释液处理过的桑叶饲喂4龄家蚕幼虫12h,96h内死亡率达100%.当浓度分别为125×10-6g/mL、250×10-6g/mL、500×10-6g/mL时,供试幼虫在72h,48h,24h内全部死亡.饲喂后24h,48h,72h的LC50值分别为0.101×10-3g/mL、0.045×10-3g/mL、0.028×10-3g/mL.     

麻疯树外植体愈伤组织中毒蛋白的Western-blot鉴定

麻疯树外植体愈伤组织中毒蛋白的Western blot鉴定戎　芳1,2,王胜华1,赵小光1,郭　亮1,魏　琴1,唐　琳1,徐　莺1,陈　放1, (1.四川大学生命科学学院,成都610064;2.安徽大学生命科学学院,合肥230039)麻疯树(JatrophacurcasL.)系大戟科麻疯树属植物,耐干旱贫瘠,抗病虫能力强[1,2],主要生长在热带和亚热带地区,在我国西南地区有丰富的资源.麻疯树种子对整体动物,如小白鼠[3]、羊[4]及人,特别是幼儿[5]表现出一定的毒性,且具有致死作用.麻疯树种子含毒成分主要是种子毒蛋白(curcin),该蛋白是一种单链核糖体失活蛋白,具抗癌活性[6],是一种具开…     

麻疯树萜醇Ⅰ的杀虫活性及其对菜粉蝶幼虫PKC活性的影响

研究了麻疯树萜醇Ⅰ对菜粉蝶幼虫的杀虫活性,并利用荧光标记多肽的非同位素检测方法首次检测了取食麻疯树萜醇Ⅰ对菜粉蝶幼虫中肠细胞内蛋白激酶C(PKC)活性的影响.结果显示:麻疯树萜醇Ⅰ对莱粉蝶幼虫无触杀活性,而具有明显的胃毒作用,处理后48h、72h和120h的LC_(50)分别为1.2753,0.8318和0.3991mg/mL.菜粉蝶幼虫对处理叶片的取食量显著少于对照组,24h和48h的拒食中浓度(AFC_(50))分别为0.3789mg/mL和0.5706mg/mL.取食麻疯树萜醇Ⅰ后,菜粉蝶幼虫体重增长显著被抑制.麻疯树萜醇Ⅰ对菜粉蝶幼虫中肠细胞内的PKC具有显著地激活作用,处理后PKC的活性逐渐上升,12h时活性为对照组的4.21倍.麻疯树萜醇Ⅰ对菜粉蝶幼虫中肠细胞PKC的激活从而导致的蛋白质磷酸化作用可能是其杀虫作用的重要机理.     

南瓜多糖对糖尿病小鼠血糖值、SOD、MDA的影响

研究南瓜多糖对四氧嘧啶型糖尿病小鼠的影响.选用昆明种小鼠腹腔注射四氧嘧啶造高血糖模型,选血糖值11.10mmol/L以上小鼠作为糖尿病小鼠随机分组,分别灌胃南瓜多糖低剂量(1mg/kg)、中剂量(3mg/kg)、高剂量(5mg/kg),2周后测血糖变化.结果表明,3组南瓜多糖灌胃剂量对四氧嘧啶型糖尿病小鼠均具有降糖作用,且灌胃剂量为中剂量(3mg/kg)时,血糖值由11.10mmol/L降至3.77mmol/L,其降糖效果最佳.研究结果还表明,灌胃南瓜多糖能提高糖尿病小鼠肝、肾、胰腺组织SOD活性,降低肝、肾、胰腺组织MDA含量.     

一个麻疯树C2H2型锌指蛋白基因JcZFP1的克隆与表达分析

从麻疯树胚乳cDNA文库中克隆得到一个C2H2型锌指蛋白基因,JcZFP1,全长931bp,开放阅读框全长735bp,编码244个氨基酸残基,等电点pI=7.0,分子量PA=27.38kD.C2H2型锌指结构位于第81至103个氨基酸残基中,包括植物锌指蛋白特有的保守氨基酸序列QALGGH.在该基因的顺式作用元件上发现了与生长发育和逆境胁迫相关的启动元件.荧光定量PCR(qPCR)检测发现,JcZFP1基因在麻疯树各器官中的表达量依次为:绿果皮花叶根茎种仁,且幼嫩器官表达量高于成熟器官;对麻疯树幼苗进行低温、干旱胁迫时,JcZFP1基因的表达量明显增加,表明该基因可能在麻疯树的生长发育及逆境胁迫应答过程中起重要作用.     

麻疯树花粉萌发条件分析及percoll分离体系的建立

为了研究离体条件下,不同培养条件对麻疯树花粉萌发的影响,取盛花期麻疯树花粉,分别在不同琼脂浓度、蔗糖浓度、温度、不同时间段下,检测麻疯树花粉萌发情况.结果表明,麻疯树花粉更易在3%的琼脂浓度、10%的蔗糖浓度、25～30℃的条件下萌发,并以此建立了优良的麻疯树花粉萌发培养基(3%琼脂+10%蔗糖+无机液体培养基).进一步研究显示,一天中的不同时间,麻疯树花粉活力不同,具有两个活力高峰期,分别为上午10～11点和下午16～17点.同时,采用Percoll密度梯度离心法对麻疯树花粉进行分离和筛选,结果表明,该方法可以有效的分离麻疯树花粉,不同层花粉活力不同,其中第六层花粉萌发率可高达90%以上,而前五层花粉萌发率均在30%以下.     

麻疯树过氧化物酶和超氧物歧化酶同工酶研究

采用聚丙烯酰胺凝胶垂直板电泳技术,对麻疯树过氧化物酶（POD）和超氧物歧化酶（SOD）同工酶酶谱进行分析,结果表明麻疯树的遗传多样性偏低.以10个麻疯树材料为试材,进行过氧化物酶（POD）、超氧物歧化酶（SOD）同工酶电泳,并应用过氧化物酶（POD）、超氧物歧化酶（SOD）同工酶对10个麻疯树材料,进行亲缘关系的鉴定,10个麻疯树材料遗传距离在0.72~1.0范围内,当遗传距离为0.8时,可以将其分为3类;而通过同工酶的方法来研究麻疯树的遗传多样性有待进一步工作来验证.     

三种常见食用蕈菌对重金属的耐受与富集研究

研究了三种食用蕈菌(金顶侧耳、黄背木耳、柱状田头菇)耐受和富集重金属Cd、Pb和Cr的能力.结果显示:金顶侧耳耐受Cr能力最强(＞800×10-3 g/kg),黄背木耳耐受Pb和Cr浓度超过400×10-3 g/kg;Cd、Pb和Cr的最大累积浓度分别是20.03×10-3 g/kg(柱状田头菇)、7.260×10-3 g/kg(黄背木耳)和31.12×10-3 g/kg(金顶侧耳).黄背木耳基部累积的三种重金属浓度均明显高于耳片,柱状田头菇和金顶侧耳菌盖部分优先累积Cd和Pb,不同浓度条件下,柱状田头菇不同部位累积Cr的能力差异较大.     

间接酶联免疫法检测麻疯树核糖体失活蛋白

建立了基于单克隆抗体的间接非竞争酶联免疫法用于检测麻疯树核糖体失活蛋白的含量.优化单克隆抗体最佳工作浓度为312.5ng/mL,辣根过氧化物酶标记山羊抗小鼠IgG最佳工作浓度为200ng/mL.该方法的线性范围为25~300ng/mL,线性回归方程为y=0.0024x+0.0322,相关系数R2=0.9985,定量检测限25ng/mL,定性检出限3.0336ng/mL.试验证明该方法准确度较高,且具有较好的重复性和特异性,可以用于实际检测麻疯树种仁核糖体失活蛋白的含量.     

麻疯树叶水浸提液对4种农作物的化感作用

采用培养皿滤纸法,研究麻疯树叶水浸提液对莴苣、油菜、玉米和小麦的化感作用.结果表明:麻疯树对作物的化感作用和浸提液浓度、生长发育期、作物种类及器官有密切关系.随麻疯树浸提液浓度增加,化感作用增强;麻疯树对4种作物的种子萌发主要表现为化感抑制作用;对莴苣地上部分的生长具有促进作用,但对其根生长产生抑制作用;其他作物的地上部分和地下部分均受到不同程度的抑制作用,部分体现了"低促高抑"的化感效应;总化感效应是萌发期幼苗期,以及莴苣油菜小麦玉米.因此在麻疯树林区适宜种植玉米.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.