对多糖测定方法的探讨

多糖是一类重要的生物大分子.综述了多糖类的常规检测和利用分子光谱法、色谱分析法、电化学分析法和生物传感器分析法对其进行分析测定的研究进展.     

基于无酶葡萄糖传感器的修饰电极及电化学性能

葡萄糖的含量检测在诸多领域都有着不可取代的作用,传感器技术也在日常生活中普遍使用,因此葡萄糖传感器成为了生物传感器研究中的热点.使用4种纳米过渡金属氧化物材料修饰的碳糊电极进行葡萄糖检测,并研究电解液浓度和修饰材料中Ni O含量的影响.研究表明,高电位处理提高了无酶电化学葡萄糖传感器的性能,为无酶电化学葡萄糖传感器的进一步应用奠定了基础.     

直接电化学酶传感器的研究进展

电化学生物传感器是电化学、电分析化学以及生物学等多学科交叉的产物,是一种全新的检测仪器,具有快速、灵敏和价廉等特点,在食品发酵工业、临床医学、环境监测、军事等领域有着重要的应用价值.简述了电化学生物传感器的基本原理和分类、电流型生物传感器的发展和特点以及直接电子转移的基本原理,并深入探讨了基于纳米材料等各种新型功能材料的直接电化学酶生物传感器的研究进展.     

石墨烯纳米复合材料在电化学免疫传感器中的应用

石墨烯纳米复合材料由于其优异的电化学性质和生物相容性被广泛的应用于制备超灵敏的电化学免疫传感器.通过与其他纳米材料复合,石墨烯的良好导电性等优点被放大,而易聚集、堆叠、生物相容性较差等缺点被克服.因而,对石墨烯的改性工作成为当下研究的热点.本文综述了石墨烯纳米复合材料在构建电化学免疫传感器中应用,包括石墨烯与金属、金属氧化物、高分子聚合物等的纳米复合材料,并对石墨烯纳米复合材料在电化学免疫传感领域的发展方向和前景做出了展望.     

基于电沉积石墨烯/普鲁士蓝/壳聚糖复合薄膜的葡萄糖生物传感器（英文）

在玻碳电极表面通过三步电沉积法制备了石墨烯/普鲁士蓝/壳聚糖复合薄膜葡萄糖生物传感器.通过循环伏安法将氧化石墨烯电化学还原,在电极表面直接得到石墨烯纳米层,在石墨烯纳米层上成功电沉积得到普鲁士蓝纳米粒子和葡萄糖氧化酶-壳聚糖复合薄膜,制备的修饰电极通过电化学方法以及扫描电镜分析了其性能与结构.在最优条件下,该生物传感器表现出了灵敏度高(50.29 mA·L·mol~(-1)·cm~(-2))、检测限低(12μmol·L~(-1))、响应时间短(3 s)等特点.电极响应电流与葡萄糖溶液浓度在0.02～10 mmol·L~(-1)范围内具有较好的线性关系.此外,在对人体血清样本的检测中,该传感器同样表现出优异的性能,对血清中常见物质具有较强的抗干扰能力.     

无机介孔材料在第三代电化学生物传感器中的应用研究

无机介孔材料是一种孔直径在2,50nm的多孔材料。这种材料具有结构规则,机械性能高、化学和热能稳定性好等特点。近年来,随着材料合成技术的提高,不断有新型高负载能力和生物相容性好的介孔材料被开发出来。同时,实验表明,无机介孔材料是固载生物大分子的良好载体。利用无机介孔材料固载氧化还原蛋白质并结合其的直接电子转移,为我们提供了一种新的构建第三代生物传感器的方法。基于无机介孔材料的特点及氧化还原蛋白质的直接电子转移,所构建的第三代电化学生物传感器有望具有较传统电化学传感器更好的性能。将介孔材料分为二氧化硅基和非二氧化硅基2大类,描述了无机介孔材料在第三代电化学生物传感器中的应用。     

先进传感器材料及其应用研究

传感器材料是传感器技术的基础,新材料的开发及应用促进了新型传感器的发展。文章扼要阐述了导电复合材料、纳米金属材料、碳纳米管材料等先进传感器材料在复合式柔性触觉传感器、电化学生物传感器、半导体气敏元件传感器中的应用。     

直接电化学酶传感器的研究进展

电化学生物传感器是电化学、电分析化学以及生物学等多学科交叉的产物,是一种全新的检测仪器,具有快速、灵敏和价廉等特点,在食品发酵工业、临床医学、环境监测、军事等领域有着重要的应用价值。简述了电化学生物传感器的基本原理和分类、电流型生物传感器的发展和特点以及直接电子转移的基本原理,并深八探讨了基于纳米材料等各种新型功能材料的直接电化学酶生物传感器的研究进展。     

酶工程的发展热点生物传感器

生物传感器的开发应归功于酶的参与。在２０世纪６０年代,科学家们将生物大分子酶与各种电化学传感器学结合起来,创造出了新的分析装置“酶电极”,它兼备了酶法分析和电极法的优点,测定迅速而准确。如由美国人首先开发研制的葡萄糖酶电极,是由葡萄糖氧化酶膜和溶氧电极构成的,能够迅速稳定人的血液和尿液中葡萄糖的含量,作为判断糖尿病患者的测试工具。 除酶之外,其他具有识别能力的生物物质如抗体、受体、细胞器等都可用作生物传感器。 生物传感器中的关键元件是生物敏感膜,即载有迅速识别被测物质并与之发生化学反应的活性物质膜…     

石墨烯-贵金属纳米复合材料在小分子电分析及电催化中的应用

综述了近年来利用石墨烯和贵金属纳米复合材料对一些常见小分子的电分析及电催化应用的主要研究进展.包括利用石墨烯-贵金属纳米复合材料构建电化学生物传感器对葡萄糖、过氧化氢等生物小分子的电分析应用,以及利用石墨烯-贵金属纳米复合材料对甲醇、乙醇和甲酸等燃料小分子的电催化应用.最后展望了该类纳米复合材料在电分析和电催化领域的应用前景和研究方向.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that