河套蜜瓜ACC氧化酶基因cDNA部分片段的克隆和序列分析

以河套蜜瓜(Cucumis melo L．ev Hetao)成熟果实为材料，用硫氰酸胍法提取总RNA，以oligo(dT)15为引物，反转录合成cDNA，利用一对甜瓜ACC氧化酶(1-aminocyclopropane-1-earboxylate oxidase，ACO)基因cDNA的特异性引物进行聚合酶链式反应(PCR)扩增。获得了预期大小的cDNA片段．将此片段克隆于pUCl9质粒中，进行DNA序列分析．序列分析结果表明该片段为545bp，编码甜瓜ACO的第126至第306个氨基酸．该河套蜜瓜ACO基因cDNA序列与Andes甜瓜、Cantaloup charentais甜瓜和哈密瓜的相应序列进行比较，其核苷酸序列与Andes甜瓜的同源性为99．4％；与Cantaloup charentais甜瓜和哈密瓜的同源性均为99．6％。与其对应的氨基酸序列的同源性均为99．4％．     

草鱼生长激素基因的克隆及原核表达研究

应用RT-PCR技术从草鱼脑垂体总RNA中克隆草鱼生长激素cDNA(cGH)，全长669bp，含开放阅读框633bp，编码210个氨基酸，分子量为23．6kDa，等电点为6．28；与已报道的草鱼GH有12个碱基、3个氨基酸残基的差异，同源性为98％．将草鱼cDNA定向插入原核表达载体pGEX-4T-1，构建了重组草鱼GH基因质粒pGEX-GcGH，经IPTG诱导，pGEX-GcGH在大肠杆菌中可表达49．6kDa的融合蛋白．     

苜蓿花叶病毒复制酶基因的克隆、序列分析及其植物表达载体的构建

以侵染苜蓿的苜蓿花叶病毒中国分离株(Alfalfa mosaic virus Chinese isolate，A1MV—Ch)RNA2为模板，利用人工合成的特异性引物，进行反转录及PCR扩增，分两段分别扩增了复制酶基因的5′端1．32kb和3′端及其非编码区的1．23kb cDNA序列．用限制性内切酶切割PCR产物后，与pUCl9重组，转化大肠杆菌DH5α，筛选重组质粒，用限制性内切酶分析及PCR鉴定，得到分别含有5′端序列和3′端序列的重组质粒。已由上述两种重组质粒构建了含有完整复制酶基因的重组质粒．进行了全序列测定，并与国外报道的A1MV-425株系的相应序列相比较，其复制酶基因编码区核苷酸序列同源性达97．8％，推测的氨基酸序列的同源性达97．6％，3′端非编码区核苷酸序列同源性为98．2％．并将全长复制酶基因与植物表达载体pROKⅡ重组，得植物转化载体pAlMV—FL．     

牦牛催乳素受体基因部分片段的克隆与序列分析

扩增出牦牛催乳素受体（尸碰尺）基因部分保守序列,为研究牦牛乳腺组织中朋三尺基因表达水平奠定基础．提取牦牛乳腺组织总RNA,根据NCB1的奶牛Jp月￡只基因cDNA序列的保守区设计特异性引物,采用RT-PCR技术扩增牦牛尸胜尺基N,结果获得577bp的片段,将该片段连接于pMD18-Simple质粒中,转化大肠杆菌,菌液PCR鉴定阳性克隆子,测序并分析氨基酸序列．分析序列与氨基酸与其他物种同源性,结果表明该序列与水牛、奶牛、绵羊、人、小鼠的尸RLR基因mRNA的对应序列的同源性分别为97．40％、99．13％、93．41％、71．07％、60．20％,编码的氨基酸同源性分别为97．40％、100％、95．83％、82-38％、72-22％．     

苹果褪绿叶斑病毒库尔勒香梨分离物外壳蛋白基因的序列分析

利用RT-PCR，获得苹果褪绿叶斑病毒库尔勒香梨分离物外壳蛋白基因的cDNA克隆。序列分析结果表明，库尔勒香梨分离物外壳蛋白基因由582个核苷酸组成，编码一个由193个氨基酸组成的蛋白质。与已报道的P863、P205、A-Ball分离物序列比较，核苷酸序列同源性为80．0％～86．1％，推导的氨基酸序列同源性为84．9％～89．6％。     

小拟南芥几丁质酶基因cDNA的克隆与序列分析

通过合成1对特异引物，应用RT-PCR技术从小拟南芥总RNA中经反转录克隆出几丁质酶基因，该cDNA基因全长985bp，含有1个963bp的开放阅读框(ORF)，编码321个氨基酸，推测分子量为35．53kD，序列同源性分析表明，该基因与拟南芥几丁质酶基因有93％的同源性。     

诸葛菜EPSPS基因5’端的克隆和序列分析

烯醇式丙酮基莽草酸—3—磷酸合成酶(5—enolpyruvylshikimate-3-phosphate synthase,EPSPS)是植物和微生物莽草酸途径中的一个必需合成酶，植物中该基因的超量表达或该基因中某些氨基酸突变可以产生对除草剂草甘膦(g1yphosate)的耐性．采用5’—RACE技术从诸葛菜(0rychophragmus violaceus)叶中克隆出一条长743bp的cDNA片段．序列分析结果表明：该cDNA与其它植物的EPSPS基因具有相当高的核苷酸序列同源性。其所编码的氨基酸序列与欧洲油菜(Brassica napus)同源性最高为90％，与拟南芥(Arabidopsis thaliana)、玉米(Zeamays)、西红柿(Lycopersicon esculentum)、烟草(Nicotiana tabacum)和水稻(Oryza sativa)的同源性分别为88％，81％，64％，62％和71％．     

青岛文昌鱼DAD1样蛋白基因的克隆和同源性分析

dud1基因为一种内源性细胞凋亡抑制子基因，在发育中可能执行着重要功能．我们通过对青岛文昌鱼18小时神经胚cDNA文库进行测序，获得文昌鱼dud1样基因的2个cDNA片断，经拼接首次得到含有完整读框的文昌鱼dud1样基因的cDNA序列，其演绎的氨基酸序列与人、鼠、鸡、非洲爪蟾、果蝇、线虫、扁豆、番茄的DAD1蛋白的同源性分别为73．5％、73．5％、67．5％、70．9％、67．5％、55．5％、41．9％、41．8％．结果证实青岛文昌鱼dud1样基因在进化上具有高度的保守性，并且在进化上更接近于脊椎动物．     

青岛文昌鱼AmphiCKS1样蛋白基因的克隆和同源性分析

通过对文昌鱼神经胚cDNA文库进行测序，获得编码文昌鱼AmphiCKS1样蛋白基因的cDNA序列，将演绎的氨基酸序列与多种脊椎动物和无脊椎动物的同源物进行比较。其同源性与人或家鼠的为77％，非洲爪蟾的为73．4％，果蝇的为77％，帽贝的为75％，线虫的为47．9％，酵母的为31．1％。结果证实在进化上介于脊椎动物和无脊椎动物之间的文昌鱼，由Amphicks 1基因所编码的AmphiCKS 1样蛋白更接近于脊椎动物；同时说明青岛文昌鱼Amphicks 1基因在进化上具有较高保守性。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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