Effects of aFGF on rat kidney in hypothermic preservation

目的:探讨酸性成纤维细胞生长因子(aFGF)对大鼠离体肾脏的低温保存效果的影响.方法:24只Wistar大鼠随机均分成高渗枸橼酸盐腺嘌呤液(HCA)对照组和aFGF复方保存液试验组:HCA液中含40 μg/L aFGF.采用腹主动脉插管原位灌注法,分别用HCA保存液和aFGF复方保存液灌洗并4℃低温保存肾脏.24、72 h后检测保存液pH值变化,以及肾脏组织丙二醛(MDA)、乳酸脱氢酶(LDH)和谷草转氨酶(GOT)水平的变化,并进行肾脏组织病理学检查.结果:肾脏低温保存24、72 h后,试验组保存液pH值均高于对照组(P＜0.05),肾组织MDA水平均低于对照组(P＜0.01);试验组肾组织LDH及GOT水平在24h和对照组无明显差异,而72 h明显低于对照组(P＜0.05).形态学观察结果显示,实验组肾脏损伤较对照组明显减轻.结论:aFGF能改善肾脏的保存效果,并可延长肾脏的保存时间.     

Effect of age on benzodiazepine-induced behavioural convulsions in rats

The benzodiazepines are a class of drugs used to alleviate anxiety. As such they constitute one of the most commonly prescribed compounds, due in part to their efficacy and safety. The physiological effect of these drugs is probably through interactions with a low affinity benzodiazepine binding site and two (types 1 and 2) higher affinity sites. The ontogenesis of these latter two binding sites in the rat differs, with the type 2 binding site being predominant at birth and the type 1 binding site increasing in number after the second week after birth. The differential development of these two receptor types is important because the immature organism may have different physiological and behavioural responses from the adult. Here we demonstrate an important difference: that a prototypic benzodiazepine, chlordiazepoxide, and a water-soluble benzodiazepine, flurazepam, produce behavioural convulsions in the preweanling rat. The convulsions are antagonized by the benzodiazepine blocker Ro-15-1788. The triazolopyridizine CL-218872, specific to the type 1 receptor, does not share this action. We suggest that this paradoxical convulsant effect of chlordiazepoxide and flurazepam is due to activation of the type 2 receptor in the absence of the type 1 receptor in the immature rat.     

硅藻土强化吸附壳聚糖固定化青霉素酰化酶

文中针对壳聚糖颗粒机械强度不够和比表面积不大的缺点,用无机多孔材料硅藻土(celite)在低压下强化吸附壳聚糖,再用戊二醛使其活化,以此为载体通过共价结合固定化青霉素酰化酶。以酶活和回收率为指标,讨论了活化、固定化及酶反应的不同条件对固定化青霉素酰化酶的酶活和回收率的影响。当戊二醛溶液质量分数为5%、pH值为8.5;酶固定化温度为27℃,固定化pH值为7.6,固定化时间为12～18h时,为最佳固定化条件。固定化酶的酶活可达10000mol/s左右,回收率约为40%。固定化酶的储存稳定性和热稳定性好,载体有良好的强度。     

米非司酮对晚孕大鼠肾超微结构的影响

目的 :探讨米非司酮对晚孕大鼠肾超微结构的影响。方法 :将妊娠 2 0dWistar大鼠 10只 ,随机分实验组与对照组 ,每组各 5只 ,灌胃法饲予实验组米非司酮 (11 5± 1 0 )mg /只 ,对照组每只给予食用麻油 1mL ,分娩后处死大鼠 ,取右侧肾脏作电镜观察。结果 :与对照组相比 ,实验组肾小体无明显改变 ,大部分肾小管上皮正常 ,局部肾小管上皮细胞出现胞质水肿 ,线粒体肿胀 ,嵴断裂 ,肾间质水肿增宽 ,间质细胞肿胀 ,甚至出现胞膜破裂、染色质边聚、核浓缩等坏死性改变。结论 :单剂量米非司酮用于晚孕引产可造成大鼠肾组织损伤。     

芒果苷对自发性高血压大鼠心脑肾组织形态学的影响

观察芒果苷对自发性高血压模型大鼠心、脑、肾等组织形态学特别是炎症损伤的影响,以自发性高血压大鼠(SHR)为研究对象,随机分为模型组、芒果苷高剂量组、芒果苷中剂量组、芒果苷低剂量组和苯那普利组,再以同龄的WKY大鼠作为正常对照。利用光镜、透射电镜观察各组大鼠的心、脑、肾等组织的形态结构。光镜结果显示:脑组织形态学未见明显改变,芒果苷高、中、低剂量组可以改善心肌炎细胞浸润及肾小球萎缩,坏死等。电镜结果显示:芒果苷可以抑制心肌细胞凋亡,改善肌原纤维排列,消除线粒体肿胀,降低系膜细胞及基底膜厚度。说明芒果苷对自发性高血压大鼠心、肾组织形态学具有改善作用,包括对炎症损伤的保护。     

光对菜豆叶枕外植体脱落的影响

通过对生理生化指标的测定,研究了光对菜豆叶枕外植体脱落的影响,证明红光抑制脱落过程是通过抑制离区组织的衰老而实现的,远红光可以促进离区组织的衰老.