Ultracentrifugation of yeast cells in a gradient of acrylamide gel and sucrose

Zusammenfassung Dichtegefälle-Ultrazentrifugation, verbunden mit Photopolymerisation von Akrylamid, ist geeignet um lebende, unverletzte Zellen unbeweglich in Bänder zu trennen, insofern sie sich punkto Gewicht, Volumen, Dichte oder Beweglichkeit im Dichtegefälle unterscheiden. Verschieden ernährte Hefezellen-Gruppen (Candida albicans) zeigten verschiedene Bandenverteilungen.     

Tissue- and stage-specific RNA patterns in Drosophila as revealed by gradient formamide gel electrophoresis

The in vitro labeled RNAs in the accessory glands from male adults, and in the salivary glands and fat body from developing larvae of Drosophila melanogaster were separated on linear gradient acrylamide gels in formamide. The patterns appear to be tissue- and stage-specific, and several lines of evidence indicate that the visualized radioactive bands include both hnRNA and messages.     

Tissue- and stage-specific RNA patterns inDrosophila as revealed by gradient formamide gel electrophoresis

Summary The in vitro labeled RNAs in the accessory glands from male adults, and in the salivary glands and fat body from developing larvae ofDrosophila melanogaster were separated on linear gradient acrylamide gels in formamide. The patterns appear to be tissue- and stage-specific, and several lines of evidence indicate that the visualized radioactive bands include both hnRNA and messages.Acknowledgments. The financial support by the Swiss National Science Foundation and the George and Antoine Claraz-Schenkung is gratefully acknowledged. I thank Prof. Max Birnstiel for providing me with the sea urchin histone mRNA and Prof. Martin Billeter for providing me with the rabbit globin mRNA as RNA markers.     

Separation of serum proteins in different amphibian species by polyacrylamide gel electrophoresis

Zusammenfassung Mittels Polyacrylamid-Gel-Elektrophorese wurden die Serumproteine von 11 Amphibienarten in 15 bis 20 Fraktionen aufgetrennt. Das elektrophoretische Muster wurde mit demjenigen des menschlichen Serums verglichen und erwies sich als artspezifisch.

---

---

This investigation was supported by grants from the Schweizericher Nationalfonds zur Förderung der wissenschaftlichen Forschung and the Georges und Antoine Claraz-Schenkung.     

Rapid quantitative evaluation of serum LDH isoenzyme patterns after agar gel electrophoresis

Resumen Las isoenzimas de la LDH se separan por electroforesis en gel de agar, y luego de colorear con una sal de tetrazolio, se cuantifican empleando una técnica rápida de elución; los resultados no difieren significativamente de los obtenidos por cuantificación densitométrica.     

Use of arrow-root powder in starch gel electrophoresis

Summary The arrow-root powder is used in electrophoresis as an alternative to potato starch. Its use is quite economical and this starch can be easily hydrolysed in any laboratory.The author is indebted to Dr M. M. Goil, Prof. & Head of the Department and Dr R. K. Sharma, Professor of Zoology, for their invaluable suggestions and help in various ways.     

Identification of human serum proteins binding iron,copper, and thyroid hormones by starch gel electrophoresis

Résumé On a analysé au moyen d'électrophorèse sur gel d'amidon les protéines qui, dans le sérum humain, lient le fer, le cuivre et l'hormone thyroïdienne, en utilisant comme témoins des protéines purifiées et la radioactivité. Le fer est lié par la -globuline qui, dans certaines familles, paraît se diviser en deux composants. Le cuivre est lié par la céruloplasmine, qui émigre dans la position ₂ rapide. La protéine principale liant la thyroxine est la préalbumine.     

Density gradient electrophoresis as a new tool in virology

Résumé Les avantages de la méthode d'électrophorèse en gradient de densité pour des travaux en virologie sont analysés.

---

---

The authors express their gratitude to ProfessorS. Gard (Karolinska Institutet, Stockholm), Dr.A. Polson (Medical School, Cape Town), and Dr.W. Bernhard (Institut du Cancer Gustave Roussy, Villejuif) for helpful criticism.We are indebted to Dr.P. Vigier (Laboratoire Pasteur de l'Institut du Radium) for Rous immune serum and to Dr.H. L. Febvre (Institut Gustave Roussy) for Rous tissue culture supernatant.     

Demonstration of polysome disaggregation due to dimethylnitrosamine by acrylamide gel polymerization after centrifugation

Résumé La désagrégation des polysomes du foie provoquée par des agents toxiques peut être mise en évidence par polymérisation, après centrifugation, d'un gradient de saccharose auquel des monomères d'acrylamide ont été additionnés. Ce procédé simple et souple donne de bons résultats et exige moins d'instrumentation que les méthodes habituelles.     

Analysis of aminotransferase in liver and serum by gel filtration

Zusammenfassung Mit der Gel-Infiltrationsanalyse von Serum und Leberfraktion normaler und CCI₄-exponierter Ratten wurde die Herkunft der Serum-Aminotransferase untersucht, was zur Annahme führte, dass seine Aktivitätssteigerung bei der Leberschädigung von der überstehenden Leberfraktion abhängt.     

Identification of proteins in human prostate tumor material by two-dimensional gel electrophoresis and mass spectrometry

Protein patterns in cells collected from benign prostatic tissues and prostate carcinomas were analyzed using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Polypeptide expression was evaluated by computer-assisted image analysis (PDQUEST). Proteins expressed by prostate tumors were identified via in-gel digestion and subsequent matrix-assisted laser desorption/ionization mass spectrometry. In addition to cytoskeletal and mitochondrial proteins, a 40-kDa protein was identified as prostatic acid phosphatase (PAP). PAP expression decreased approximately twofold between benign and malignant tissue. Increased expression of heat shock protein 70 and decreased expression of tropomyosin 1 were also observed in the malignant tissue. The analysis of prostate material by two-dimensional gel electrophoresis and mass spectrometry shows that particular proteins are of interest as markers of disease. Received 18 December 2000; accepted 4 January 2001     

A rapid micro radial electrophoretic method of protein separation on cellulose acetate membranes

Summary A new rapid micromethod for protein separation under a radial electric field is described. As many as 12 rabbit serum samples could be separated in 4–6 min.The authors are thankful to Dr P.K. Ramachandran, Defence Research and Development Establishment, Gwalior, for the sustained interest and helpful suggestions.     

Inhibition of respiration ofAspergillus oryzae by adsorption of the mycelium on cellulose acetate fibres

Résumé L'intensité de respiration d'Aspergillus oryzae est fortement diminuée par adsorption du mycélium à un filtre d'acétate de cellulose soigneusement nettoyé. Cet effet suggère que certaines substances d'une importance capitale dans le métabolisme sont éliminées de la surface des hyphes.