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1.
采用B5和MS相组合的BM基本培养基和适宜浓度的激素(2,4-D,6-BA和GA3)能解除红豆杉种胚的休眠.单因子试验和不同组合的正交试验结果显示:单独使用2,4-D0.1mg/L,6-BA2mg/L和GA32mg/L均在一定程度上促使红豆杉种胚的萌发,萌发率分别为70%,55%和28.6%;最佳正交组合为6-BA1mg/L+2,4-D0.1mg/L+AC4g/L,萌发率达81%. 相似文献
2.
荞麦组织培养中的植株再生 总被引:1,自引:0,他引:1
用荞麦无菌苗的幼茎和幼叶作外植体分别接种在MS补加不同激素组合的培养基上培养获得再生植株。实验结果表明,适宜诱导愈伤组织的激素组合为2.4-D4.0mg/L+KT0.2mg/L,适宜愈伤组织生长的激素组合为2.4-D1.0mg/L+KT1.0mg/L。诱导芽的较适宜的组合为6-BA3.0mg/L+IAA0.1mg/L,不定芽接种到含MS+IBA2.0mg/L+KT0.1mg/L的MS培养基上就能生 相似文献
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骆驼刺高效离体植株再生体系的建立 总被引:4,自引:1,他引:3
通过对络驼刺(Alhagi pseudalhagi Desv)不同外植体来源、不同培养基及激素配比的比较,建立骆驼刺培养高效再生植株实验体系。表明骆驼刺下胚轴切段在含1.5 ̄2.0mg/L2,4-D和0.5 ̄1.0mg/L 6-BA的MS培养基中100%诱导愈伤组织,在含1.5mg/L 6-BA和1.0mg/L NAA的MS培养基上愈伤组织分化成苗,转至含2.0mg/L IBA和0.2mg/L NAA的MS培养基上成根得到完整再生植株,组织学观察表明植株再生主要为器官发生途径,染色体稳定遗传。 相似文献
5.
雪兔子叶的组织培养及植株再生 总被引:1,自引:0,他引:1
以雪兔子无菌苗的幼叶为外植体,接种到MS补加NAA(1.0-2.0)mg/L、6-BA(0.1-1.0)mg/L的培养基上诱导愈伤组织,其中以MS+NAA2.0mg/L+6-BA0.5mg/L的诱导效果最好,经6周培养即可形成大量的黄绿色愈伤组织,诱导率可达100%,经2-3次继代培养后,将生长发良好的愈伤组织转接到MS附加6-BA(0.5-2.0)mg/L、IAA(0.05-0.1)mg/L、N 相似文献
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以巴西铁(Dracaena sanderiena)茎段为接种材料,MS为基本培养基,吲哚乙酸(IAA)、茶乙酸(NAA),2,4-二氯苯氧乙酸(2.4-D)、6-苄氨基嘌呤(6-BA)、6一糠基氨基嘌呤(6-FA)、吲哚丁酸(IBA)、生根粉(ABT1)、玉米素(ZT)为附加成分,进行诱导、分化、生根试验。诱导结果表明:IAA不起作用,2,4-D有较好的促进作用,0.5mg/L(2,4-D)+2mg/L(6-BA)组合,诱导率高达90%。分化结果表明:NAA、6-BA效果较好,6-FA作用不明显,lmg/LNAA+4ms/L(6-BA)+2ms/LZT组合,分化率最高.达81.1%。生根结果表明:MS+0.5mg/LNAA+0.5mg/L(6-FA),MS+0.4mg/LNAA+2mg/LIBA,MS+0.2mg/LNAA+0.1mg/L(6-FA)+ABT1三种培养基均发根,生根率70%以上,根粗,但有点脆,若培养基中加入活性碳产生的根则细而韧。 相似文献
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建兰簇生原球茎的诱导及分化诸因素研究 总被引:2,自引:0,他引:2
以建兰的腋芽为外植体,诱导出了簇生原球茎.B5和MS培养基均适宜,在1~5mg/LNAA和0.5~3mg/LBA的范围内,激素含量高,有利于簇生原球茎的诱导.激素浓度高于4mg/L,能形成假根茎.假根茎和簇生原球茎均能分化出营养芽和花芽.花芽在连续光照下,可在试管内开花.椰乳亦是原球茎诱导的促进物质,以8%~15%的浓度为宜.1mg/LBA和0.6mg/L4PU-30能促进原球茎的分化.活性炭对防止褐化,促进根系生长是有用的 相似文献
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风信子外植体植株再生系统的研究 总被引:8,自引:1,他引:7
风信子叶片及鳞片直接诱导下定芽的培养基为MS附加6-BA2.5mg/L、NAA2.0mg/L、IAA0.5mg/L和MS附加6-BA2.5mg/L、NAA2.5mg/L。不定芽发育成小鳞茎的培养基为MS附加GA0.2mg/L、IAA0.5mg/L。鳞茎移栽成活率为90%,从而建立了风信子植株再生系统。 相似文献
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油菜萝卜胞质不育系恢复材料Ad-6(F4)测交二代恢复株TCF2,在MS附加5mg/L 6-BA+0.5mg/L NAA,3mg/L,6-BA+0.2mg/L NAA,3mg/L 6-BA|0.3mg/L NAA+600mg/L水解5mg/L-6-BA+0.3mg/L NAA+5mg/L AgNO3对TCF2诱导丛生芽有较好的效果。 相似文献
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播娘蒿子叶及下胚轴诱导再生植株的研究 总被引:1,自引:0,他引:1
用揪娘嵩种子,在附加6-BA0.5~1.0mg/L+NAA0.1mg/L的MS培养基上获得无菌苗,幼苗的下胚轴在含6-BA0.1mg/L+NAA0.5mg/L的MS培养基上的出愈情况最佳,将来自下胚轴的愈伤组织培养了含有6-BA2.0mg/L+NAA0.2mg/L的MS培养基上分化出的丛生芽状态最好,最佳生根培养基为1/2MS+IBA0.5mg/L+NAA0.05mg/L。 相似文献
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Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness. 相似文献
12.
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly. 相似文献
13.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。 相似文献
14.
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well. 相似文献
15.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation. 相似文献
16.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death. 相似文献
17.
Miao Maosheng 《华南师范大学学报(自然科学版)》2014,(6)
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula- 相似文献
18.
Yang Weitao 《华南师范大学学报(自然科学版)》2014,46(6):137-138
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re- 相似文献
19.
Bernard Kirtman 《华南师范大学学报(自然科学版)》2014,(6)
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that 相似文献
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《上海师范大学学报(自然科学版)》2014,(5):550
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with 相似文献