Serum and liver glutamate oxalacetate transaminase in hepatectomized rats

Zusammenfassung Auf subtotale Hepatektomie (5. Tag) erfolgende Leberregeneration führt zu einer wesentlichen Erhöhung des SGOT-Wertes, mit Anstieg bis zum 10. Tag. Damit parallel verläuft auch die Transaminase-Aktivität der regenerierenden Leber.     

Changes in transglutaminase activity in carbon tetrachloride-damaged rat liver

Summary A significant decrease in transglutaminase (TGase) activity was observed in the cytosol and nuclear fractions of carbon tetrachloride-damaged rat liver. The degree of decrease in TGase activity in the cytosol fraction was closely related to the serum transaminase level. Gel filtration studies revealed that TGase activity in 80 kDa fractions significantly decreased, but that in 160 kDa fractions slightly increased after carbon tetrachloride treatment.     

Changes in protein-bound sulfhydryl group concentration in the liver of rats fed cabbage

Zusammenfassung Eine statistisch signifikante Erhöhung der eiweissgebundenen SH-Stoffe, somit auch der totalen SH-Stoffe in der Leber mit strumigenem Kohl gefütterter Ratten, wurde festgestellt. Im Gehalt nicht eiweissbundener SH-Stoffe (SH-glutathion) in Leber und Blut nach Kohlverfütterung kam es zu keiner Veränderung.     

Changes in transglutaminase activity in carbon tetrachloride-damaged rat liver.

A significant decrease in transglutaminase (TGase) activity was observed in the cytosol and nuclear fractions of carbon tetrachloride-damaged rat liver. The degree of decrease in TGase activity in the cytosol fraction was closely related to the serum transaminase level. Gel filtration studies revealed that TGase activity in 80 kDa fractions significantly decreased, but that in 160 kDa fractions slightly increased after carbon tetrachloride treatment.     

Changes in cholinesterase activity of muscle after crushing the sciatic nerve of rats

Summary The subcellular distribution of cholinesterase (ChE) was studied in the gastrocnemius muscle of rats after strong or weak nerve crushing. The ChE activities of muscle were decreased to a greater extent by strong crushing than by weak crushing. In particular, the ChE activity of the fraction containing sarcoplasmic reticulum was most greatly decreased. These results suggest that the change in the ChE activity of the microsomal fraction most finely reflects the strength of nerve crushing.Acknowledgments. This study was accomplished in Central Research Laboratories, Sankyo Co. Ltd. The authors wish to thank Prof. Tsuneyuki Nakazawa, Department of Neuropsychiatry, School of Medicine, Fujita-Gakuen University, and Dr Yutaka Sakai for their valuable advice and support, Sr Jean M. Michalec for her critical comments, and Miss Hamako Katano, Mr Naoji Mikuni and Miss Yoshie Ishii for their skillful technical assistance.     

Changes in cholinesterase activity of muscle after crushing the sciatic nerve of rats

The subcellular distribution of cholinesterase (ChE) was studied in the gastrocnemius muscle of rats after strong or weak nerve crushing. The ChE activities of muscle were decreased to a greater extent by strong crushing than by weak crushing. In particular, the ChE activity of the fraction containing sarcoplasmic reticulum was most greatly decreased. These results suggest that the change in the ChE activity of the microsomal fraction most finely reflects the strength of nerve crushing.     

Effect of wall-degrading enzymes on protoplasts transaminase activity

Résumé Les enzymes employées pour l'obtention enzymatique des protoplastes de racines (Allium) altèrent les transaminases (EC. 2.6.1.1.) de protoplastes mécaniquement préparés. Cette inhibition transaminasique est partiellement levée lorsque les protoplastes ne sont plus en contact avec ces enzymes. Les effets des enzymes dégradant les parois cellulaires sont discutés relativement aux propriétés biochimiques des protoplastes.     

Sex difference in liver tryptophan pyrrolase activity of starved rats

Zusammenfassung Die Tryptophanpyrrolase (LTP) ausgewachsener weiblicher Ratten nimmt während 4-stündiger Hungerperiode von 1,06µM Kynurenin auf 2,26µM/g/h zu. Sie erreicht nach 24 h wieder Normalwerte, um dann während einer 8tägigen Hungerperiode auf 5,75µM/g/h anzusteigen. Die LTP männlicher Ratten zeigt nur unbedeutenden Anstieg während gleicher Hungerperioden.     

Histochemistry of glutamic-oxaloacetic transaminase in mouse liver during MHV-3 infection

Riassunto Gli autori hanno studiato istochimicamente il comportamento dell'attività GOT nel fegato di topi infettati con virus MHV-3. Alla 48° ora dall'infezione si ha un notevole aumento dell'attività enzimatica, strettamente legato alla necrosi parenchimale. Tale aumento è maggiore nei fegati con necrosi focale che non in quelli con necrosi sub-massiva. Vengono discussi i reperti istochimici e le loro correlazioni con i valori dell'attività GOT nel siero degli animali infettati e con i dati biochimici riportati da altri Autori.

---

---

The skilled technical assistance ofMr. R. Gentile is gratefully acknowledged.

---

In this paper the following abbreviations are used: GOT, glutamicoxaloacetic transaminase; GPT, glutamic-pyruvic transaminase.     

Sarcosine dehydrogenase activity in liver mitochondria of infant and adult rats

Zusammenfassung Die Aktivität der Sarcosinedehydrogenase in Lebermitochondrien 7tägiger Ratten ist um 50% kleiner als in adulten Tieren. Dieser Unterschied ist nicht durch das Elektrontransportflavoprotein limitiert und kann auch nicht durch eine Änderung in dem relativen Wert der löslichen Proteine in den Mitochondrien erklärt werden.     

Starvation-induced adaptation of rat liver tyrosine transaminase and serine dehydrase

Résumé Les activités de la tyrosine transaminase et de la sérine déhydrase sont augmentées dans le foie de rats normaux ou surrénalectomisés soumis au jeûne. Ces augmentations, qui sont supprimées par la puromycine ou le glucose, ne sont dues ni à une sécrétion d'hormones surrénaliennes ni à une accumulation des substrats des enzymes.

---

---

This work was supported by a grant (AM 07 249 02) from the U.S. National Institutes of Health. Technical assistance of MmeYveline Van Heijenoort and MileCristiane Portemer (C.N.R.S.) is gratefully acknowledged.     

Inhibitory effect of fasting on the glucagon-induced increase of liver phosphorylase A activity in rats

Summary The increase of liver phosphorylase A activity observed 1, 2 and 3 min after i.v. administration of 0.1 g kg^–1 glucagon to fed rats was found to be completely absent in 24 h fasted animals, although there is even an exaggerated liver cAMP response to glucagon after fasting.Acknowledgments. We would like to thank Mrs M. Fritzová and Mr F. Kovai for expert technical assistance.