Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain

Ha-Ras augments c-Jun-mediated transactivation by potentiating the activity of the c-Jun activation domain. Ha-Ras also causes a corresponding increase in phosphorylation of specific sites in that part of the c-Jun protein. A Ha-Ras-induced protein kinase cascade resulting in hyperphosphorylation of the c-Jun activation domain could explain how these oncoproteins cooperate to transform rat embryo fibroblasts.     

Hedgehog signalling activity of Smoothened requires phosphorylation by protein kinase A and casein kinase I

The Hedgehog (Hh) family of secreted proteins governs cell growth and patterning in animal development. The Hh signal is transduced by the seven-transmembrane protein Smoothened (Smo); however, the mechanism by which Smo is regulated remains largely unknown. Here we show that protein kinase A (PKA) and casein kinase I (CKI) regulate Smo cell-surface accumulation and activity in response to Hh. Blocking PKA or CKI activity in the Drosophila wing disc prevents Hh-induced Smo accumulation and attenuates pathway activity, whereas increasing PKA activity promotes Smo accumulation and pathway activation. We show that PKA and CKI phosphorylate Smo at several sites, and that phosphorylation-deficient forms of Smo fail to accumulate on the cell surface and are unable to transduce the Hh signal. Conversely, phosphorylation-mimicking Smo variants show constitutive cell-surface expression and signalling activity. Furthermore, we find that the levels of Smo cell-surface expression and activity correlate with its levels of phosphorylation. Our data indicate that Hh induces progressive Smo phosphorylation by PKA and CKI, leading to elevation of Smo cell-surface levels and signalling activity.     

国际大科学研发协同中的文化冲突与合作模式创新研究

国际大科学的发展促进了世界各国之间的科学合作研发,整合各国研发优势和先进技术,共同研发与探索,但鉴于各国存在地域、文化、社会等方面的差异,在合作研发过程中,不可避免的存在各种问题。基于国际大科学研发协同的背景,探讨参与大科学项目中各国存在的文化冲突问题以及合作过程中采取的合作模式,深入研究国际合作中的文化冲突,提出国际大科学背景下研发协同中,消除文化冲突,创新合作模式的对策建议。     

Interaction of c-Abl and p73alpha and their collaboration to induce apoptosis.

c-Abl, a non-receptor tyrosine kinase, is activated by agents that damage DNA. This activation results in either arrest of the cell cycle in phase G1 or apoptotic cell death, both of which are dependent on the kinase activity of c-Abl. p73, a member of the p53 family of tumour-suppressor proteins, can also induce apoptosis. Here we show that the apoptotic activity of p73alpha requires the presence of functional, kinase-competent c-Abl. Furthermore, p73 and c-Abl can associate with each other, andthis binding is mediated by a PxxP motif in p73 and the SH3 domain of c-Abl. We find that p73 is a substrate of the c-Abl kinase and that the ability of c-Abl to phosphorylate p73 is markedly increased by gamma-irradiation. Moreover, p73 is phosphorylated in vivo in response to ionizing radiation. These findings define a pro-apoptotic signalling pathway involving p73 and c-Abl.     

基于连续精细化分层广播编码的接收机协作策略及性能分析

在广播系统中用户间协作能有效改善系统的期望失真性能,但是在传统的协作方式(放大转发,解码转发)中,由于中继转发了对目标节点无用的信息,会造成系统额外的转发功率开销.针对此问题,提出基于连续精细化分层广播编码的接收机协作策略,利用连续精细化分层广播编码的结构特点,中继可以选择目的节点所需要的信息进行转发,在达到与解码转发协作方式相同的系统期望失真性能的同时,降低了中继节点的转发功率.     

针灸穴位治疗眩晕63例疗效观察

眩晕是临床上常见的症状。作者运用针灸疗法对63例眩晕症患者进行治疗,取得了较好的疗效,治愈率为79．4％,好转率为15．9％,总有效率为95.2％。本文对眩晕的诊断标准、辩证施沽原则、效疗评定标准和疗程作了统一规定,另外,对一例典型病例作了详细的报告,最后对眩晕的发病机理和经验作了更深入的阐述。     

华庆地区长6_3储层成岩作用与成岩相研究

三叠系延长组长63段是鄂尔多斯盆地华庆地区重要的勘探目的层,也是典型的低孔-低渗储层.文章对长63砂岩的成岩相进行了初步研究,划分出碳酸盐胶结成岩相、绿泥石胶结成岩相、硅质充填及次生加大胶结成岩相、长石溶蚀成岩相.分析了成岩相与储层发育的关系及其区域分布规律,为进一步的岩性———地层油藏的成藏、规律分析和储层预测提供了依据.     

Al63-xCu25Fe12Yx稀土准晶组织性能研究

研究了在雾化制备Al63-xCu25Fe12Yx稀土准晶粉末,结果表明制备的准晶粉末细小且近似球形,添加少量稀土的准晶粉末更细小;850 ℃×4小时热压后准晶中除少量Y的稀土化合物相外,基本上是准晶相;添加少量的稀土后,准晶的硬度和耐磨性均有一定程度提高.     

促进知识互补效应的我国产学研合作科研创新激励机制研究

基于知识互补视角,将委托-代理分析引入到产学研合作科研创新激励机制的问题研究当中,探究影响产学研合作活动行为选择的主要激励因素,提出相应的针对性措施和建议,以增强合作活动中对互补性知识提供方的激励强度与自由程度。     

黄芪注射液治疗肺心病急性加重期73例疗效观察

目的:探讨黄芪注射液静脉注射治疗肺心病急性加重期的疗效.方法:对照组67例,采用常规的抗感染、平喘、强心及利尿等治疗;治疗组73例,在常规治疗的基础上同时加用黄芪注射液40mL溶于5%葡萄糖250mL中静脉滴注,每日一次,10d为一疗程.结果:治疗组和对照组的有效率分别为91.78%和74.63%(P<0.01);两组治疗后心输出量(CO,L/minm2)、心脏指数(CI)、左室射血分数(LVEF)、左室短轴缩短率(FS)、每搏输出量(SV)、二尖瓣快速充血期和心房收缩期血流速度(E/A)均较治疗前明显改善(P<0.05,P<0.01),但治疗组更优于对照组(P<0.05).两组动脉血气治疗后均有改善,但治疗组改善程度优于对照组(P<0.05).结论:黄芪注射液对肺心病急性加重期具有较好疗效.     

浙江加强与两院合作中科院杭州科技园开园

4月8日,中国科学院杭州科技园隆重开园。中国科学院杭州科技园于2002年11月1日由中国科学院上海分院、杭州市科技局、杭州高新技术产业开发区管委会以及杭州机床集团有限公司共同创建。目前,中国科学院与其他单位合作,在科技园内设立了半导体照明研发中心、数控机床研发中心、能源环保研发中心,并对进入产业化阶段的科研成果进行中期研究开发,其中在研项目7个。目前计划入园的高科技企业12家,孵化企业2家。科技园的开园,意味着中科院与地方共建的首家以市场机制运作的科技园区将正式投入运行。■浙江加强与两院合作 中科院杭州科技园开园…     

高校产学合作教育问题与对策研究

作为一种特色的育人模式,产学合作教育通过安排学生到生产一线顶岗实习,培养学生较强的分析问题和解决问题能力,从而达到全面提高大学生综合素质的目的。研究分析高校产学合作教育过程中出现的问题,进而探索相对应的解决措施,提供理论上的支持,有助于高校产学合作教育的顺利开展和高素质综合型人才的培养。     

肺压宁胶囊治疗慢性阻塞性肺病73例疗效观察

目的:观察纯中药复方制剂———肺压宁胶囊(CapFYN)对慢性阻塞性肺病(COPD)的长期疗效。方法:选取确诊的COPD患者73例,分为治疗组42例,对照组31例。两组基础治疗相同(均不使用氨茶碱),治疗组用肺压宁胶囊每次4粒,每天3次;对照组用硝苯吡定片剂10mg每天3次,对比观察治疗20周前后患者的咳嗽、咳痰、神疲、乏力等症状,舌腹静脉瘀血征,肺功能(1s用力呼气容积/用力肺活量即FEV1/FVC)以及平均肺动脉压的组内和组间变化,并计算它们的危险度(Risk)、相对危险度(RR)、相对危险度减少(RRR)和绝对危险度减少(ARR)值,以评价治疗效果。结果:随访率分别为98%和99%;神疲、乏力症状、瘀血征、肺功能、肺高压4项结果的RR值均>1,症状的RRR为-0 476,余3项的RRR值均<-0 5,4项的ARR值依次为26 11%、43 80%、24 29%、22 39%;两组患者肝肾功能及血、尿常规均无明确改变。结论:肺压宁胶囊是一种长期服用方便、安全,能改善病人症状、瘀血征和气道气流阻塞及降低肺动脉高压的纯中药复方制剂,可作为COPD患者综合治疗的一种辅助方药。     

探讨民族汽车工业中产学研的合作模式

近年来,自主创新逐渐被社会各界重视并成为民族企业生存发展的生命源泉,各个行业尤其是民族汽车工业掀起了产学研合作高潮。文章介绍了江淮汽车在双边或多边产学研合作的实际运作模式,分析了"全程参与"合作模式的良好效果和推广意义,用理论和实例相结合的方法阐述了产学研合作在民族汽车工业的运用。     

High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor.

Nerve growth factor (NGF) interacts with two different low-affinity receptors that can be distinguished by affinity crosslinking. Reconstitution experiments by membrane fusion and transient transfection into heterologous cells indicate that high-affinity NGF binding requires coexpression and binding to both the low-affinity NGF receptor and the tyrosine kinase trk gene product. These studies reveal a new growth factor receptor-mediated mechanism of cellular differentiation involving trk and the low-affinity NGF receptor.     

Altered tyrosine 527 phosphorylation and mitotic activation of p60c-src.

The tyrosine kinasee activity of p60c-src, the protein product of the c-src gene, increases during mitosis; this may be important in initiating at least some of the cellular changes that occur during this phase of the cell cycle. Although there is evidence that p60c-src is phosphorylated at several sites during mitosis, phosphorylation in vitro does not increase its kinase activity. We now report that the kinase activity of a p60c-src mutant with residue tyrosine 527 changed to phenylanine does not change during the cell cycle, suggesting that changes in the phosphorylation state of this residue may be responsible for the activation of p60c-src at mitosis. Although changes in phosphorylation at Tyr 527 cannot be detected with the wild-type protein we find that phosphorylation at Tyr 527 of a mutant with reduced kinase activity decreases threefold during mitosis. On the basis of these results we suggest that activation of p60c-src at mitosis results from decreased phosphorylation on Tyr 527, and that p60c-src may be or may activate the kinase that phosphorylates Tyr 527.