丁鱼岁遗传多样性的随机扩增多态性DNA分析

利用RAPD分子标记技术对15个丁鱼岁养殖个体的遗传多样性进行分析.选用20个随机引物对其基因组DNA进行PCR扩增,有16个引物可以扩增出稳定且清晰的条带,其中S103、S104和S105为多态引物.16个引物共扩增出65个DNA位点,片段大小在200~3 000 bp之间,其中多态性位点6个,多态位点比例为9.23%.个体间遗传距离在0~0.067 4之间,平均遗传距离为0.028 6.结果显示,丁鱼岁养殖群体的遗传多样性水平较低.     

金佛山特有杜鹃花遗传多样性分析

利用ISSR分子标记初步分析了重庆金佛山特有的4种杜鹃花的亲缘关系,从100个引物中筛选出13个能扩增出清晰条带并具有多态性的引物.共扩增出了239条DNA片段,分子量约为200~1500bp.其中多态性片段约为171条,平均每条引物扩增出18.38条DNA片段.同时采用DPS软件计算出4种杜鹃花的Nei’S遗传距离,并用NTSYSpc2.1中的UPGMA法构建了系统树.结果表明4种杜鹃花之间既有相同的遗传背景,又存在一定的差异.4种杜鹃花明显分为两类:树枫杜鹃为一类,疏花美容杜鹃,金佛山美容杜鹃与阔柄杜鹃为一类.     

蜡梅品种的RAPD分析

从200个随机引物中筛选出16个多态性稳定的引物,对38个蜡梅品种进行遗传多样性和亲缘关系RAPD分析.共扩增出154条DNA片段,其中,多态性DNA带98条,占总扩增片段的63.6%.应用Popgene32软件进行Nei相似性系数和遗传距离计算,并利用UPGMA法构建聚类树状图,把供试的38个蜡梅品种分为7个大类,聚类结果与形态分类结果基本一致.结果表明,RAPD标记可用于蜡梅品种鉴定和亲缘关系探讨.     

东方百合杂交系部分栽培品种的遗传多样性分析

利用125条多态性随机引物对市场上具有代表性的10个东方百合杂交系栽培品种的遗传多样性进行了分析.结果表明：在125条引物中,30条引物能够扩增出多态性片段,引物多态性比例为24%.引物扩增片段数目从3到13条,其中S223可以完全区分10个供试品种.UPGMA法聚类结果表明,10个供试品种之间遗传距离为0.0930～05349,10个供试品种在相似系数0.57处可以分为3类,品种之间遗传变异比较丰富.其中Bernini和Tiber的遗传距离是0.0930,推测两者杂交亲本十分相近;而Aktiva与其它     

20个红花品种资源的ISSR分析

目的:利用ISSR标记技术分析国内20个主要红花品种遗传多样性,为红花种质资源选育提供参考资料.方法:选用ISSR分子标记法对不同地理区域的20个红花品种基因组DNA进行PCR扩增.结果:20个ISSR引物共产生382条扩增带,其中多态性带366条,多态性条带比例为95.42%.10个品种间的遗传相似系数分布在0.615 2~0.790 6之间,说明不同红花品种间遗传多样性较为丰富.聚类分析结果表明,利用ISSR技术可将来自不同地区的20个红花品种分为5个主要类群.其中,杞县刺红花单独为一类群.其余四个类群均未表现出品种遗传多样性与地理分布的相关性.     

极危物种——南川木波罗遗传多样性ISSR分析

本研究利用ISSR-PCR分子标记技术分析研究了重庆市七个地区野生生长的46株南川木波罗(Artocarpus nanchuanensis)样品的遗传多样性.利用筛选出的12条UBC引物共扩增出了117条DNA片段,其中多态性片段为113条,多态条带百分率为96.58%.NTSYS~pc2.1软件分析表明:七个地区南川木波罗之间的遗传相似性介于0.062~0.989之间,并用UPGMA法构建了系统树.结果表明该市野生生长的南川木波罗既有相同的遗传背景,又存在较明显的差异.46份材料划分为4类,聚类基本符合地理来源相近的材料聚为一类,呈现出一定的地域性分布规律.     

基于ISSR的披针莲座蕨居群遗传多样性分析

采用ISSR分子标记对披针莲座蕨的6个自然居群97个样品进行了DNA多态性分析.从38个随机引物中筛选出11个有效引物,扩增共产生81条DNA片段,其中79条为多态性条带,多态位点百分比(PPB)为97.5%.与其他蕨类植物相比,披针莲座蕨居群遗传多样性水平较高.居群遗传关系聚类结果与各居群的地理分布基本一致.     

怀地黄85-5品种内遗传多样性的RAPD和ISSR分析

采用CTAB法提取了怀地黄85-5品种16个单株的基因组DNA,使用紫外分析和琼脂糖凝胶电泳检测其纯度和大小,利用RAPD及ISSR分析其品种内遗传多样性.从24个RAPD引物和43个ISSR引物中分别筛选出具有稳定多态性条带的RAPD引物3个和ISSR引物2个,分别对16个单株基因组DNA进行PCR扩增.3个RAPD引物共扩增出7条带,其中多态性条带为4个,多态性比率为57.14%.2个ISSR引物共扩增出17条带,其中多态性条带为11个,多态性比率为64.7%.结果表明,怀地黄85-5品种内存在遗传差异,但遗传多样性比地黄品种间的低.     

31种石斛属植物的RAPD遗传多样性分析

采用随机扩增多态性DNA技术对31种石斛属植物的遗传多样性进行分析.从100条RAPD引物中共筛选出18条引物,18条引物共扩增出1802条带,其中多态性位点有261个,多态性比为99.2％.UPGMA 聚类结果显示,基因型之间的相似系数分布于0.642～0.838之间,31种石斛属植物样品分为7类.聚类分析显示供试样...     

川渝部分地区野生黄鳝RAPD引物筛选及其在绵阳群体的验证

采用RAPD技术对产自四川绵阳、内江、雅安和重庆忠县的24尾野生黄鳝基因组DNA进行引物筛选,40条随机引物中有4条引物扩增出片段。选择在4个地区黄鳝个体间多态性较丰富的RAPD引物A10在绵阳黄鳝群体进行扩增分析。RAPD引物A10在6个绵阳黄鳝个体的扩增,产生比较清晰的条带并且呈现一定的多态性,绵阳黄鳝群体内的平均遗传相似率为0.8947,群体内的平均遗传距离为0.1053,从一定程度反映出绵阳地区黄鳝较低的遗传多样性。本研究所筛选引物可为黄鳝分子标记和遗传多样性的分析、亲缘关系和种质的鉴定以及遗传资源的保护利用提供参考。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with