毕赤酵母热致死动力学模型的建立与检验

毕赤酵母作为最成熟的蛋白质过量表达平台微生物之一,广泛应用于酶制剂、功能性大分子等大宗产品的发酵制造.建立毕赤酵母常规温度下(50~60,℃)的热致死动力学模型,有助于指导毕赤酵母发酵制造体系的菌体后处理工艺,增加菌体利用价值.为了建立毕赤酵母在50~60,℃条件下热致死动力学模型,将不同菌体量的毕赤酵母菌液在50、55、60,℃条件下进行不同时间热灭活处理,通过平板培养活细胞计数的方法评价不同处理方式下毕赤酵母的热致死效果,得到的致死曲线用Weibull模型和多元回归方程进行拟合构建热致死动力学模型,并对建立的模型进行数学检验和实用性检验.结果表明:建立的毕赤酵母热致死动力学模型可靠,具有实际使用价值.本研究所建立的模型能较好地模拟不同温度、不同处理时间对不同菌体量的毕赤酵母热致死效果的影响.并可以为毕赤酵母热灭活处理条件的确定提供计算依据.     

多台系统异步可靠性增长模型及其统计分析

在各单台系统Weibull过程的可靠性增长假设条件下,建立了一种多台系统异步可靠性增长模型,并给出了可靠性增长试验数据的统计分析方法,包括模型参数的点估计、区间估计、系统MTBF估计、增长趋势检验及模型拟合优度检验等.较好地解决了不同步可靠性增长试验数据的统计分析问题.     

提高庆大霉素产生菌F-212孢子萌发率的研究

本文研究了三十一种孢子萌发因子对庆大霉素产生菌棘孢小单孢菌F—212的孢子萌发率和芽长的影响。分别进行了单因子、双因子及四因子试验,并通过正交试验找出了促进孢子萌发的最适剂量组合,为进一步提高庆大霉素发酵水平创造了条件。     

Weibull统计特性风速条件下的自然通风实验

采用双参数Weibull统计模型对室外逐秒风速进行了拟合,结合频谱特性分析对实测自然风的典型性进行验证,并考虑到风向对通风效果的影响定义了风向因子参数。通过实际自然风条件下模型比例为1∶10的通风实验,对尺寸因子、形状因子及风向因子等自然风特性参数对自然通风效果的影响进行了研究,并建立了相应的影响模型。研究结果表明:逐秒自然风风速统计分布服从双参数Weibull统计模型;实际自然风条件下,通风效果随Weibull分布的尺寸因子及风向因子的增加而加强,随形状因子的增加而减弱。     

华北落叶松人工林直径分布预测模型构建

【目的】基于不同直径分布预测模型(Weibull分布模型、Gamma分布模型、Lognormal分布模型),构建包含华北落叶松林分因子的直径分布线性混合效应模型,有助于分析直径分布对林分因子动态变化的响应。【方法】利用塞罕坝华北落叶松人工林标准地调查数据,应用最大似然估计法(Maximum Likelihood Estimation, MLE)估计模型参数,通过K-S(Kolmogorov-Smirnov)检验、C-V(Cramer-von Mises)检验、A-D(Anderson-Darling)检验对模型适用性进行检验,基于最优模型构建华北落叶松人工林直径分布线性混合效应模型。【结果】塞罕坝华北落叶松人工林直径分布最优模型为Weibull分布;基于最优模型,构建了包含优势高、断面积、对数密度的线性混合效应模型,当3个参数随机效应方差-协方差结构和误差项结构均为对角矩阵结构[UN(1)]时,模型的拟合效果最好。包含位置、尺度、形状3参数随机效应项模型的决定系数R²分别为0.895、0.888、0.801,均方误差(MSE)分别为5.365、1.724、1.151,均方根误差(RMSE)分别为2.316、1.313、1.073,拟合结果均较好。【结论】线性混合效应模型具有较好的预测直径分布能力,可为精准预测华北落叶松人工林直径分布提供理论依据和技术参数。     

基于3次样条插值的数控机床几何与热复合定位误差建模

通过分析机床整个温升直到热平衡的误差数据,总结误差分布的数学规律,将热误差和几何误差分离,运用基于压紧样条条件下的3次样条插值算法,以线性拟合后的余差为建模数据,建立了数控机床几何与热复合定位误差数学模型.实验结果表明,该数学模型能很好地拟合数控机床定位误差曲线,补偿后数控机床定位精度提高了80%以上.该方法可运用于不同时刻或不同机床温度下的机床定位误差补偿,建模原理明了、过程快速,模型适用性好.     

废弃菌体水解液作氮源发酵产腈水合酶的研究

工业生产中微生物细胞在发酵结束后常被作为废弃物丢弃,容易造成环境污染和资源浪费.对废弃细胞进行破壁处理,废弃菌体水解液可替代酵母膏作有机氮源用于发酵产腈水合酶.通过采用超声波、冻融、热碱3种方法破碎细胞,测定水解液中氨基氮含量的高低,确定选择热碱法为最佳细胞破碎方法.通过正交实验选出最优破壁条件为:N aOH加入量5g.L-1,水解温度100℃,水解时间1.5h.在最佳破壁条件下,细胞破碎液中氨基氮含量为24.5 g· L-1.并考察了酵母膏与水解液的不同配比对腈水合酶发酵酶活的影响,确定了酵母膏与废弃菌体水解液的最佳配比为3:2.实验结果表明:利用热碱法处理废弃菌体的水解液作为氮源部分代替酵母膏,发酵酶活为1 063万U·mL-1,而完全利用酵母膏作为氮源的发酵酶活为1 055万U·mL-1.     

蜡蚧轮枝菌对茶蚜的侵染模型

接种4株蜡蚧轮枝菌Lecanicillium,(Verticillium,) lecanii(菌株V07、V08、V23和V27)不同剂量(2x103、2×104、2×105、2 x106和2 x107孢子- mL-1)孢子悬浮液到茶蚜Toxoptera aurcntii室内种群,观察茶蚜种群在24℃和100% RH培养条件下的感染和发病情况,得到蜡蚧轮枝菌侵染茶蚜的发病指数,用Gompertz模型拟合了病情指数和作用时间及初始接种剂量之间的动态关系.结果表明:表观侵染速率与初始接种浓度相关,同一菌株侵染速率随初始接种孢子浓度的增大而增大.初始接种剂量为2×107孢子.mL-1时,菌株V07对烟粉虱的侵染速率最快.     

区间删失数据下Weibull 比例优势模型的参数估计

基于区间删失数据建模是当前复杂数据分析的热点之一。本研究在两类区间删失数据下建立Weibull比例优势模型,基于极大似然估计给出了模型参数,进一步讨论了估计量的渐近性质。数值模拟验证了模型参数的估计效果,并将提出的模型及方法应用到艾滋病临床试验数据和肺肿瘤试验数据中,给出了感兴趣事件的生存函数曲线,通过与生存函数的非参数极大似然估计比较,表明所提方法具有较好的拟合效果。     

生长曲线模型在隧道监控数据处理中的应用

分别采用Weibull生长曲线模型、对数函数模型、指数函数模型和幂函数模型等4种回归模型对某隧道拱顶下沉的实测数据进行了回归分析,并将各模型预测结果和实测数据进行了对比.结果表明：Weibull生长曲线模型的预测结果与实测数据最为吻合,精度最高,误差最小.因此,采用Weibull模型对隧道围岩变形情况进行回归分析,效果要优于其他3种传统模型.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that