瓦松的CAM活性与NAD—苹果酸酶的关系

对专一性ＣＡＤ植物瓦松的ＣＡＭ活性与ＮＡＤ－ＭＥ的关系进行了初步研究，结果表明，瓦松的ＭＡＤ－ＭＥ有明显的昼夜调节特性，其活性昼高夜低；同时，该活性还存在季节性变化，在７月酶活性可达最高峰。其酶的最适ＰＨ为７，最适温度为５５℃。     

瓦松和长药景天NAD－苹果酸酶的比较研究

通过对专性ＣＡＭ植物瓦松（Ｏｒｏｓｔａｃｈｙｓｆｉｍｂｒｉａｔｕｓ）和兼性ＣＡＭ植物长药景天（Ｓｅ－ｄｕｍｓｐｅｃｔａｂｉｌｅ）的ＮＡＤ－苹果酸酶进行比较研究，在反应系统含５ｍｍｏｌ／ＬＭｎＣｌ＿２，５０μｍｏｌ／ＬＣｏＡ，５ｍｍｏｌ／Ｌ　ＤＴＴ，ｐＨ值为７ｈ，其苹果酸饱和曲线均为双曲线，且瓦松的ＮＡＤ－苹果酸酶对苹果酸极为敏感。光对该酶活性无明显影响。该酶对温度较敏感，其最适温度为５５℃。晴天的酶活明显比阴天高；即使在阴天，瓦松的酶活也较高。在同一温度条件下，瓦松的酶活高于长药景天。     

瓦松和长药景天NAD—苹果酸酶的比较研究

通过对专性ＣＡＭ植物瓦松和兼性ＣＡＭ植物长药景天的ＮＡＤ－苹果酸酶进行比较研究，在反应系统含５ｍｍｏｌ／Ｌ ＭｎＣｌ２，５０μｍｏｌ／Ｌ ＣｏＡ，５ｍｍｏｌ／Ｌ ＤＴＴ，ｐＨ值为７ｈ，其苹果酸饱和曲线均为双曲线，且瓦松的ＮＡＤ－苹果酸酶对苹果酸极为敏感。光对该酶活性无明显影响。该酶对温度较敏感，其最适温度为５５℃。晴天的酶活明显比阴天高；即使在阴天，瓦松的酶活也较高。在同一温度条件下，瓦松的酶活高     

木霉4131菌株纤维素酶的分离纯化和部分性质研究

绿色木霉（Ｔｒｉｃｈｏｄｅｒｍａ Ｖｉｒｉｄｅ）突变菌株４１３１＾［１］产生的纤维素酶经过ｓｅｐｈａｄｅｘＧ－７５和ＤＥＡＥ－ｓｅｐｈａｄｅｘ－Ａ－２５两种层析分离,分离得到具有内切α－葡聚糖酶（ＣＭＣａｓｅ）活性的纯组分,提纯后酶的比活力提高到原来的２６．２倍;回收率为３２．８％,分子量为５６９００,等电点为４．０,最适反应温度为５５℃,最适ｐＨ为４．５,金属离子Ｋ＾＋,Ｎａ＾＋,Ｍｇ＾２＋对其     

几种沙生植物光合碳代谢途径关键酶的研究

研究了分布于甘肃省临泽地区的沙拐枣、珍珠、霸王、泡泡刺、胡杨等５种沙生植物的光合酶：果糖－１．６－双磷酸酯酶（ＦＤＰａｓｅ）、１．５－二磷酸核酮糖羧化酶（ＲＵＢＰｃａｓｅ）、磷酸烯醇式丙酮酸羧化酶（ＰＥＰｃａｓｅ）、ＮＡＤ－苹果酸脱氢酶（ＮＡＤ－ＭＤＨ）活性的季节变化。结果表明，珍珠和沙拐枣的ＦＤＰａｓｅ酶活性远低于其它几种材料；珍珠、霸王、沙拐枣的ＰＥＰｃａｓｅ活性明显高于其它两种材料；珍珠、沙拐枣的ＮＡＤ－ＭＤＨ活性明显高于其它三种材料。可见，珍珠、霸王是ＣＡＭ植物，沙拐枣是Ｃ４植物，胡杨是Ｃ３植物，泡泡刺有可能在干旱协迫下由Ｃ３向Ｃ４或ＣＡＭ途径转化。     

绿色乳杆菌α－羟基戊二酸脱氢酶的纯化和特性研究

本文对绿色乳杆菌（Ｌ．Ｖｉｒｉｄｅｓｃｅｎｓ）Ｂ１７５产生的α－羟基戊二酸脱氢酶用硫酸铵盐析；ＤＥＡＥ—纤维素柱层折；超滤浓缩；苯基琼脂糖凝胶（ＰｈｅｎｙｌＳｅｐｈａｒｏｓｅ）疏水层析等方法纯化，使酶的纯度提高１１７倍。通过ＨＰＬＣ和等电聚焦测得该酶的分子量约为６６，０００，等电点为４．２。酶催化作用的最适ｐＨ５．５，最适温度为６０℃，对α—酮戊二酸和ＮＡＤＨ的Ｋｍ值分别为０．１４×１０（－３）Ｍ和０．０３×１０（－３）Ｍ。     

植物钙调素的分离纯化及其特性研究

本文以烟草愈伤组织和花椰菜这花序为材料，分离纯化钙调素。所得ＣａＭ的纯度用ＳＤＳ－ＰＡＧＥ鉴定；生物活性用其对牛心肌环腺苷酸磷二酯酶（ＰＤＥ）的激活作用测定（ＣａＭ）的产量用酶联免疫吸附法（ＥＬＩＳＡ）测定。并比较了上Ｐｈｎｅｙｌ－ＳｅｐｈａｒｏｓｅＣＬ－４Ｂ柱前对ＣａＭ溶液的不同处理。纯化的烟草愈伤组织和菜花ＣａＭ，具有牛脑ＣａＭ所特有的一些性质。它对牛心肌ＰＤＥ有明显激活作用，在含有Ｃａ＾２＋     

DEPENDENCEOFFLUXCREEPTIMEt0ONSAMPLESIZEFORYBa2Cu3O7

ＤＥＰＥＮＤＥＮＣＥＯＦＦＬＵＸＣＲＥＥＰＴＩＭＥｔ０ＯＮＳＡＭＰＬＥＳＩＺＥＦＯＲＹＢａ２Ｃｕ３Ｏ７ＤｉｎｇＱｉＸｕＪｉａｎｊｉａｎ（ＩｎｓｔｉｔｕｔｅｏｆＡｐｐｌｉｅｄＰｈｙｓｉｃｓ，ＤｅｐａｒｔｍｅｎｔｏｆＰｙｓｉｃｓ，ＮａｎｊｉｎｇＵｎｉｖ...     

HARMONIC AC SUSCEPTIBILITY FOR TEXTURED YBa_2Cu_3O_7

ＨＡＲＭＯＮＩＣＡＣＳＵＳＣＥＰＴＩＢＩＬＩＴＹＦＯＲＴＥＸＴＵＲＥＤＹＢａ２Ｃｕ３Ｏ７ＧｅＹｏｎｇ１）ＤｉｎｇＳｈｉＹｉｎｇ２）（１）ＤｅｐａｒｔｍｅｎｔｏｆＰｈｙｓｉｃｓ,ＮａｔｉｏｎａｌＬａｂｏｆＳｏｌｉｄＳｔａｔｅＭｉｃｒｏｓｔｒｕｃｔｕｒ...     

THE INITIAL－BOUNDARY VALUE PROBLEM FOR THE SYSTEM OF THE SECOND ORDER COMPLEX EQUATIONS

ＴＨＥＩＮＩＴＩＡＬ－ＢＯＵＮＤＡＲＹＶＡＬＵＥＰＲＯＢＬＥＭＦＯＲＴＨＥＳＹＳＴＥＭＯＦＴＨＥＳＥＣＯＮＤＯＲＤＥＲＣＯＭＰＬＥＸＥＱＵＡＴＩＯＮＳＱｉｕＦｅｎｇ（邱峰）；Ｇｕｏｙｕｚｈｕ（郭玉柱）（ＢｅｉｊｉｎｇＮｏｒｍａｌＵｎｉｖｅｒｓｉｌｙ，...     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that