Development of the hypothalamic-hypophysial-gonadotrophic activities in fetal rats

Summary Pituitary responsiveness to LHRH and anti-LHRH serum was investigated in fetal rats aged 18.5–22.5 days. Synthetic LHRH injection in utero into fetuses brought about a remarkable depletion of pituitary-LH with a corresponding increase of serum-LH on day 18.5. On the contrary, anti-LHRH serum administration to day-20.5 fetuses caused a significant augmentation of pituitary-LH 1 day later. These data indicate that LH-gonadotrophs respond to LHRH even on day 18.5, and that endogenous LHRH begins to affect LH-gonadotrophs on day 20.5.This work was supported by a grant from Ministry of Education, Science and Culture, Japan. The exert technical assistance of Mr Yoshihito Okamura is gratefully acknowledged.     

Hexobarbital action in rats with flavonoid-deficiency

Summary In flavonoid-deficient rats, hexobarbital-sleeping times are prolonged. The hexobarbital concentrations in brain, liver and plasma are increased. These results are discussed as a consequence of an impaired drug metabolism.     

Development of the hypothalamic-hypophysial-gonadotrophic activities in fetal rats

Pituitary responsiveness to LHRH and anti-LHRH serum was investigated in fetal rats aged 18.5-22.5 days. Synthetic LHRH injection in utero into fetuses brought about a remarkable depletion of pituitary-LH with a corresponding increase of serum-LH on day 18.5. On the contrary, anit-LHRH serum administration to day-20.5 fetuses caused a significant augmentation of pituitary-LH 1 day later. These data indicate that LH-gonadotrophs respond to LHRH even on day 18.5, and that endogenous LHRH begins to affect LH gonadotrophs on day 20.5.     

Persistance of maternally derived3H-estradiol in fetal and neonatal rats

Summary Injection of³H-estradiol into pregnant rats resulted in fetal blood radioactivity 5 times higher than in maternal blood. Significant amounts of³H-estradiol were found in fetal blood 24 h later and in the offspring 5 days after birth.Supported by NIH Grant HD 08844.     

Prolonged action of drugs in rats with flavonoid-deficiency

Summary In flavonoid-deficient Wistar-rats, the action of caffeine, harmine, hexobarbital, morphine and pentobarbital is enhanced. In contrast, the thiopental sleeping time is shortened. These observations may indicate impaired drug metabolism as a consequence of the flavonoid-deficiency state.