Sperm chymotrypsin-like enzymes of different inhibitor-susceptibility as lysins in ascidians

Summary Inhibitory effects of three peptidyl phenylalaninals on fertilization and on chymotrypsin-like enzyme activity of sperm in three species of ascidians were examined. The results suggest that a sperm chymotrypsin-like enzyme is indispensable for the fertilization in each of the ascidians, and that these enzymes have different susceptibilities to inhibitors.12 November 1986Acknowledgments. We are indebted to Dr M. Hoshi of Tokyo Institute of Technology for his helpful discussion. This work was supportd by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan.     

Isolation of different thermophilic enzymes fromBacillus stearothermophilus

Zusammenfassung Es wird eine Reinigungsmethode beschrieben, die es erlaubt, aus einemB. stearothermophilus Zellaufschluss fünf thermophile Enzyme in reiner Form zu isolieren und weitere zwei Enzyme stark anzureichern.     

On the presence of different histidine decarboxylating enzymes in mammalian tissues

Zusammenfassung Säugetiergewebe enthalten mindestens zwei verschiedene, Histidin-decarboxylierende Fermente. Das eine, wahrscheinlich mit Dopadecarboxylase identisch, scheint für die Histaminbildung unter physiologischen Bedingungen ohne Bedeutung zu sein. Das andere ist in Mastzellen und wachsenden Geweben lokalisiert.

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This work has been supported by grants from the Swedish Medical Research Council.     

Manipulating the activity of immobilized enzymes with different organo-smectite complexes

Summary Enzymes may be immobilized on hydrophobic surfaces of organo-smectite complexes. The immobilized enzyme may be active, partially active, or inactive depending on the nature of the organic surface. These materials may be useful as models for enzymes in natural systems, and in medicine and industry.This work was partially supported by the National Science Foundation under Grant No. CHE-8306583. Journal Article No. 11603 of the Michigan Agricultural Experiment Station.     

Vanadium biochemistry: The unknown role of vanadium-containing cells in ascidians (sea squirts)

Summary This article reviews several new developments in vanadium biochemistry, as elucidated from studies of ascidians. A hypothesis correlating ascidian blood cell function to anaerobiosis, via two prominent redox constituents, namely vanadium(III) and the tunichromes, a family of metal ion complexing/reducing hydroquinonoid peptides, is presented.     

Vanadium biochemistry: the unknown role of vanadium-containing cells in ascidians (sea squirts)

This article reviews several new developments in vanadium biochemistry, as elucidated from studies of ascidians. A hypothesis correlating ascidian blood cell function to anaerobiosis, via two prominent redox constituents, namely vanadium(III) and the tunichromes, a family of metal ion complexing/reducing hydroquinonoid peptides, is presented.     

Intracellular enzymes in renal lymph as a measure of anoxic injury of the kidney

Zusammenfassung Nachweis, dass 30-minutige Nierenischämie zum Austritt von Enzymen des Zellplasmas aus den Zellen führt. Nach 120 min Nierenischämie kommt es auch zum Austritt von lysosomalen und mitochondrialen Enzymen aus den Zellen.     

Activity of phospholipases A and some lysosomal enzymes in rat testes at different stages of hypervitaminosis A

Summary Excess of vitamin A induces decrease of neutral phospholipase A₁ and A₂ activity in rat testes homogenates on the 4th day, and increase of -galactosidase activity on the 8th day of treatment. It is suggested that phospholipase A activity decrease is of great importance in development of testicular disorders, caused by disbalance of vitamin A.     

Activity alterations of metabolic enzymes in the anterior pituitary of female rats during acute and chronic starvation,as well as after refeeding

Summary The activity of glycolysis and hexose monophosphate shunt decreases while the activity of some oxydative enzymes and acid phosphatase increases in the anterior pituitary of adult female rats during starvation. The alterations depend on the severity of starvation. The polypeptide hormone production also decreases. A close relationship exists between the metabolic activity of the gland and its endocrine function.     

Collagenolytic enzymes in human serum

Zusammenfassung Mit Hilfe des synthetischen Kollagenasesubstrates PZ-Pro-Leu-Gly-Pro-D-Arg wurden im humanen Serum kollagenolytische Enzyme nachgewiesen, deren Spezifität der Clostridiopeptidase A entspricht. Die Spaltungsgeschwindikeit zeigt in Abhängigkeit von pH ein Optimum bei pH 7,2 und bei pH 8,0. Das spricht für die Existenz zweier Enzyme. Zur quantitativen Bestimmung der Enzymaktivitäten wurde eine Methode ausgearbeitet, mit der eine Reihe von normalen humanen Seren analysiert wurde.     

Sperm bioassay for detecting acute toxicity of chemical pollutants

Summary A starfish sperm bioassay for detecting chemical pollutants is described. KCN and HgCl₂ inhibited O₂ consumption of spermatozoa by 50% at 0.07 ng/ml and 0.3 ng/ml respectively.I am grateful to Dr B. Hart for advice and supervision.     

Histochemistry of lysosomal enzymes in juxtaglomerular cells

Zusammenfassung In den Granula juxtaglomerulärer granulierter Mäusenierenzellen wurde eine saure Phosphatase und eine-Glykuronidase-Aktivität nachgewiesen. (Methoden für Arylsulphatase und unspezifische Esterase waren negativ.) Es wird der Zusammenhang mit der Lysosomenart der Granula diskutiert.