Synthesis of sulfated glycosaminoglycans by the three cell types of the rabbit cornea in culture.

Rabbit corneal cells were cultivated for 21 days and then exposed to Na235SO4, a precursor of sulfated glycosaminoglycans (GAG). All 3 cell types of the cornea, the fibroblasts, the epithelial as well as the endothelial cells, synthesize GAG. The fractionation-patterns of the epithelial and endotherlial GAG are almost identical and differ clearly from the one of fibroblastic GAG.     

Excessive proliferation of epithelium of the rabbit cornea

Zusammenfassung Die Schädigung der Basalmembran durch eine Stichverletzung hat eine Proliferation und Einwanderung von Corneaepithelien zur Folge. Eigenartigerweise sind bei diesem Prozess keine Mitosen nachweisbar, was darauf hinzuweisen scheint, dass Amitosen experimentell verfolgt werden können.

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This research was supported in part by grants from the National Research Council (Canada) and the National Cancer Institute of Canada.     

The potency of heparin-like activity of glycosaminoglycans released by human endothelial cells in culture

Summary Glycosaminoglycans isolated from culture medium conditioned by human endothelial cells showed heparin-like antithrombin III cofactor activity measured by Xa inhibition. Their activity was relatively weak, 0.1% of the potency of heparin, but was approximately 5-fold more potent than that of glycosaminoglycans derived from vascular smooth muscle cells.     

The potency of heparin-like activity of glycosaminoglycans released by human endothelial cells in culture

Glycosaminoglycans isolated from culture medium conditioned by human endothelial cells showed heparin-like anti-thrombin III cofactor activity measured by Xa inhibition. Their activity was relatively weak, 0.1% of the potency of heparin, but was approximately 5-fold more potent than that of glycosaminoglycans derived from vascular smooth muscle cells.     

Neuronal types in long-term culture of avian retina

Zusammenfassung Morphologische Befunde an Nervenzellen der Retina von Hühnerembryonen werden mitgeteilt. Mit der Bodian-Silberimprägnation in Langzeit-Gewebekulturen konnten multipolare Ganglienzellen, bipolare und Horizontalzellen, Amakrine und Synapsen identifiziert werden.

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This work was supported by the Medical Research Council of Canada.     

Synthesis and transport of cholesterol in aortic cells: Radioisotopic studies in cell culture

Zusammenfassung Synthese und Transport von Cholesterin in Hühner-Aortazellen wurden untersucht: 1. grösste Inkorporationsrate von C¹⁴-Acetat in der Sterin-Fraktion, 2. verminderte Aufnahme von C¹⁴-Cholesterin in Aortazellen nach ihrer Inkubation mit lipämischem Menschenserum. Der Eintritt von H³-Cholesterin in das Zytoplasma der Aortazellen von Affen wurde autoradiographisch festgestellt.     

Some common features of three types of insect sensilla

Zusammenfassung In den letzten Jahren wurde gezeigt, dass um den distalen Teil der Dendriten von mechano- und chemorezeptorischen Sinneszellen eine Hülle vorkommt. Ihr möglicher Einfluss auf die gemeinsamen elektrophysiologischen Merkmale der Sinnesorgane wird diskutiert.     

Biochemical evidence for two types of noradrenaline storage particles in rabbit iris

Summary Centrifugation techniques were used to determine the subcellular distribution of noradrenaline (NA) and dopamine -hydroxylase (DH) in the rabbit iris. By application of isopycnic and differential gradient centrifugation methods 2 types of NA vesicles could be demonstrated. Of the total particle bound NA about 70% is associated with light and about 30% with heavy vesicles. For both types of vesicles the distribution of DH reflected that of NA.     

Ultrastructural aspects of the cell fusion induced by Visna virus on sheep choroid plexus cell in culture]

Sheep choroid plexus cells infected with low multiplicities of infection of Visna Virus were stellate and had long and thin processes containing filaments and forming cytoplasmic bridges between adjacent cells. Enlargement of the bridges resulted in the formation of multinucleated cells. Some glycoproteins were clustered on filaments outside the cell. The cytoplasmic changes showed : an intensive protein synthesis; numerous mitochondria closely associated with filaments and some lysosomes and numerous vesicules near the plasma membrane.     

Neurosecretory cell types in normal taste bud

Riassunto Nella presente nota vengono esaminate da un punto di vista ultrastrutturale particolari cellule presenti nel calice gustativo normale, per alcuni aspetti assai simili alle cellule del I tipo e contenenti numerose vescicole di tipo neurosecretorio.