高通量、多组分表面等离子体谐振生化分析系统研究

基于表面等离子体谐振(surface plasmon resonance,SPR)原理,利用图像分析技术和自动进样技术,研制成一种新型高通量、多组分图像SPR生化分析系统;对微阵列SPR敏感芯片进行动态检测,实现高通量、多参数、多组分快速检测和定量分析．以15单元的阵列芯片为例,对兔IgG和人IgG分别与羊抗兔IgG和羊抗人IgG的免疫结合反应进行了实验检测．结果表明,该分析系统可进行实时高通量、多参数检测,具有灵敏度高、免标记等优点,而且阵列芯片中所设置的参比单元可以消除溶液本体折射率和温度变化的影响,提高了测量精度和准确性;另外芯片可以再生重复使用,降低了测试成本．     

基于ARM的印版密度检测系统设计

为及时纠正印刷错误, 提高印刷质量, 构建了一个基于 ARM(Advanced RISC Machines)芯片的印版密度检测系统。 该系统可使印刷过程中的密度检测数字化、 自动化和系统化。 在硬件上采用 IMAGERECEIVER 作为图像采集模块, 使用嵌入式 CMOS(Complementary Metal Oxide Semiconductor)图像采集(OV511+OV7620)芯片的 USB 摄像头作为图像采集设备; PPI-DMA(Pixel Per Inch-Direct Memory Access)作为高速数据传输通道; LCD(Liquid Crystal Display)作为图像显示器; Keyboard 作为人机交互设备。 软件上, 首先对采集图像进行灰度化、二值化, 而后进行网点面积计算, 最终实现印版网点密度检测的功能。 测试结果表明, 该系统能实现印版密度检测。     

基于FPGA平台的实时人眼检测系统设计

采用近红外光源与图像差分相结合的方法在现场可编程逻辑阵列(FPGA)平台上实现了人眼检测系统。利用图像差分可以获得具有亮瞳孔效应、以人脸为主体的目标图像,通过对其进行灰度拉伸、分割、积分投影可以得到人脸位置,在检测到的人脸区域利用改进的复杂度算法实现了人眼检测。经测试,系统的处理速度可达30帧/秒,满足实时性检测的要求。基于近红外光源的方法消除了外界光源的干扰,从而保证了系统的鲁棒性。     

ITO电极图案自动检测系统的总体设计

介绍了一种基于规则的ITO (透明导电薄膜 )电极图案的自动检测系统 ,检测过程主要分为两个步骤 ,第一阶段是对图像进行预处理和图像标记 ,第二阶段根据特定的知识和规则对图像进行学习检测 .并着重讨论了系统的软件组成及实现原理     

电视信号监测与报警系统的设计和实现

提出了使用信号检测和图像分析技术监测电视信号的中断、非法插播和干扰的方法和系统设计。该系统采用FPGA和视频采集卡实现32-64路同步信号时序检测与图像检测,并依此设计出一个电视信号自动监测与报警系统;论文介绍了相应的硬件模块设计和软件流程,精确图像模板的动态更新方法,以及通过台标图像模板实现对电视台标的检测过程;实验结果表明,系统达到实用要求。     

选煤厂自动冲洗控制系统

将PLC与WinCC组态相结合,建立了自动冲洗控制系统。上位机的WinCC组态监控软件实现了冲水过程的图像实时监控,下位机可编程控制器(PLC)实现对自动冲洗系统各个执行机构的控制。应用结果表明,本控制系统使用方便,运行可靠。     

表面等离子体共振谱仪用于糖蛋白检测研究

介绍了一种表面等离子体共振(SPR)生化分析仪,采用角度扫描与强度调制相结合的方法来实现对糖蛋白的实时监测.在糖蛋白的检测实验中,对不同pH的再生液进行对比,pH=3的再生能力达到97%,选择该pH的溶液作为再生液;对同一浓度的糖蛋白进行5次测量,得出相对标准偏差(RSD)为3.83%;通过非糖蛋白与糖蛋白的对比实验,说明本实验方法可以对糖蛋白进行特异性检测;最后对浓度在0.01~1mg/mL的糖蛋白进行检测,并进行了线性拟合和多项式拟合,通过残差分析和相关系数的比较得出RNase B的浓度与归一化光强的关系更符合多项式拟合,因此以多项式拟合曲线作为其标准曲线.实验结果表明该SPR谱仪可以对糖蛋白进行特异性检测,该技术将会在医学方面得到广泛应用.     

表面等离子共振检测葡萄糖浓度的数据处理方法

根据表面等离子共振(SPR)检测葡萄糖浓度时信号和噪声的特点,研究了寻找曲线最低点算法和基线动态调整方法等SPR数据后处理方法.采用加权质心法精确地捕获到了表面等离子共振曲线的最低点,采用固定计算点数的动态基线法调整基线位置,消除了基线附近点值的波动对测量结果的影响.模拟仿真及实验验证结果表明,采用固定点数的动态基线法和加权质心法处理SPR数据,能显著提高SPR检测葡萄糖的分辨率,在葡萄糖浓度检测技术中有很好的应用前景.     

一种新型电子警察的设计

为了有效解决现有电子警察系统中存在的缺陷,提出一种新型电子警察的设计方法.在硬件上基于高速模拟电路、高速DSP和大规模可编程逻辑器件设计高清摄像机,在软件上采用检测区域、虚拟线和背景帧差相结合的检测方法,并结合全天候图像亮度特征信息分析,实现了车辆实时检测与违章记录的有效采集的性能要求.实验结果表明通过新方法设计的系统稳定可靠,实时性强,检测率高,记录有效率高,完全满足应用要求.     

连接条件对GFRTP自冲铆接性能影响的数值研究

采用LS-DYNA软件建立玻璃纤维增强热塑性复合材料(GFRTP)的自冲铆接(SPR)有限元模型,对SPR过程进行数值模拟和实验验证。通过比较应力分布、钉腿张开度及接头强度,分析底座几何形状及铆钉材料对GFRTP工件SPR过程及接头强度的影响。研究结果表明:与金属SPR接头钉腿被下工件完全包裹而形成自锁结构不同,GFRTP工件SPR接头的形成机理是钉腿穿透下工件而向外翻卷,与钉头一起形成机械锁。连接条件对GFRTP工件SPR性能有显著影响。在一定范围内,增大底座凸台倾角有助于提高SPR的自锁性能;增加铆钉材料强度,可有效提升SPR接头的强度。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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