Effects of puromycin on rat embryos in vitro

Summary Somite-staged rat embryos were exposed to varying concentrations of puromycin for 48 h in vitro. Medium concentrations below 0.92 M had no significant effects, while concentrations above 1.84 M were lethal. Between these extremes, there were concentration dependent increases in the incidence of malformations in a close relationship to growth retardation.     

Separation of germ layers in presomite rat embryos

Résumé Les feuillets du cylindre-Æuf du rat ont été disjoints par une solution composée de deux enzymes: 0,5% de trypsine et 2,5% de pancréatine. La séparation a été achevée par une aiguille de tungstène. Les feuillets ainsi détachés retiennent leur pouvoir de croissance et de différenciation comme homogreffes, sous la capsule rénale.     

Effects of puromycin on rat embryos in vitro

Somite-staged rat embryos were exposed to varying concentrations of puromycin for 48 h in vitro. Medium concentrations below 0.92 microM had no significant effects, while concentrations above 1.84 microM were lethal. Between these extremes, there were concentration dependent increases in the incidence of malformations in a close relationship to growth retardation.     

Weight of rat embryos after X-ray irradiation

Résumé Les embryons de rat ont été irradiés par les rayons X à la dose de 100 r du cinquième au dixième jour de la gestation. Tous les embryons ont été fixés au boulin le quinzième jour de la gestation et ont été pesés après le passage dans l'alcool.Les embryons irradiés, comme c'est déjà connu, sont plus légers que les témoins, mais il y a aussi une différence significative entre les divers groupes irradiés.     

Effects of beta-hydroxybutyrate on rat embryos grown in culture

In order to investigate further the relationship between maternal diabetes and fetal malformation, rat embryos were grown in vitro in the presence of beta-hydroxybutyrate, one of the ketone bodies produced by diabetics. At 10 mM, beta-hydroxybutyrate produced minor abnormalities and at 20 mM it produced major abnormalities in rat embryos.     

Effects of streptozotocin on early rat embryos grown in culture

Summary Addition of 1 mg/ml streptozotocin to serum in which 10-day rat embryos are cultured reduces their growth and viability. There is therefore a risk that administration of this drug to pregnant animals to induce diabetes could also have direct, deleterious effects on the embryos.     

Effects of streptozotocin on early rat embryos grown in culture.

Addition of 1 mg/ml streptozotocin to serum in which 10-day rat embryos are cultured reduces their growth and viability. There is therefore a risk that administration of this drug to pregnant animals to induce diabetes could also have direct, deleterious effects on the embryos.     

Autoradiographic studies on the rat embryos in the pre-implantation stages

Zusammenfassung Die Rattenblastocyste synthetisiert, kurz bevor sich die Membrana pellucida auflöst, Nukleinsäuren, wie aus der Aufnahme von Uridin durch die Zellkerne elektronenmikroskopisch und autoradiographisch gezeigt wurde. Bei verzögerter Implantation wird Nukleinsäure in geringerem Ausmass aufgebaut.     

Abnormal development of cultured rat embryos in rat and human sera prepared after vitamin A ingestion

Summary Human and rat sera were assayed for teratogenic activity using a whole rat embryo culture technique. Sera prepared from blood withdrawn 1–5 h after the ingestion of vitamin A capsules caused developmental retardation and craniofacial abnormalities. Control sera permitted normal growth and differentiation.Financial support for this project was provided by Department of Energy contract EVO 3139 (Office of Health and Environmental Research), USA (Scientific contribution 940, Storrs Agricultural Experiment Station), and by the Medical Research Council, U.K.     

Human serum as a culture medium for rat embryos

Summary A comparison was made between the development of post-implantation rat embryos in human serum and rat serum. Protein synthesis (growth) and somite number (differentiation) were retarded in human serum and there was an increased frequency of neural tube defects. Male and female human sera supported development equally well.     

Human serum as a culture medium for rat embryos

A comparison was made between the development of post-implantation rat embryos in human serum and rat serum. Protein synthesis (growth) and somite number (differentiation) were retarded in human serum and there was an increased frequency of neural tube defects. Male and female human sera supported development equally well.