Corpus luteum function in ageing inbred mice.

Impaired breeding performance of aged female mice was associated with reduced numbers of ovulations and increased mortality of embryos. The amounts of progesterone in the sera, corpora lutea and uterine flushings of these animals were similar to those of young animals when measured by radioimmunoassay.     

Corpus luteum cells grown as monolayers

Zusammenfassung Die Fähigkeit der Corpus-luteum-Zellen als Monolayers zu wachsen wurde untersucht. Die von Schwein und Ratte entnommenen Gelbkörper wurden zerkleinert und mit einer 0,25% igen Trypsinlösung in eine Suspension übergeführt. Die Kulturen wurden bis zum 8. Tag beobachtet. Die wachsenden Luteinzellen zeigen die typische Form und auch die ausgeprägte Aktivität der ⁵-3-OH Steroiddehydrogenase.     

Genotype dependence of monoamine oxidase in inbred strains of mice

Summary MAO activity was found to be influenced by the genotype or strain of mouse up to 20 days of age. The strain differences observed may derive from different rates of brain development. A number of neurological mutations comprizing three pathological classes had no effect on MAO.This work was supported in part by NIH grant No. NS06448 from the National Institute of Neurological Disease and Stroke.     

Pathological changes in inbred strains of mice following early thymectomy and irradiation

Summary Mice subjected to thymectomy and irradiation were found to develop a range of pathological change in various organs. These changes were accompanied by antibodies to a variety of self-components. The pattern of pathological and autoimmune change was found to vary with the strain. This strain-related expression did not appear to be associated with the major histocompatibility complex (H-2).This study was partly supported by the National Health and Medical Research Council of Australia.Author for correspondence and reprint requests.     

Effects of d-amphetamine upon open field behaviour in two inbred strains of mice

Résumé Chez des souris C57BL/10J, on constate une augmentation de l'activité locomotrice durant tous les 7 jours qui suivent leur injection avec la d-amphétamine. Chez des souris BALB/cJ, cette augmentation n'apparaît pas, sauf légèrement après le quatrième jour. Des facteurs génétiques jouent un rôle important dans la détermination de la réponse comportementale à la d-amphétamine.

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This research was funded by National Institutes of Mental Health grant No. MH-18517-01. Facilities were provided by the Maryland Psychiatric Research Center, Baltimore, Maryland.     

Regional brain [Met]-enkephalin in alcohol-preferring and non-alcohol-preferring inbred strains of mice

Summary Scrutiny of the data from these studies reveals that the C58/J alcohol-preferring mice have significantly lower baseline methionine-enkephalin levels in both the corpus striatum and hypothalamus compared to C3H/CHRGL/2 non-alcohol-preferring mice. In other brain regions in these two strains, specifically, pituitary, amygdala, midbrain, and hippocampus, analysis of methionine-enkephalin levels did not show any significant differences. This suggests that the hypothalamus may indeed be a specific locus involved in the regulation of alcohol intake, via the molecular interaction between neuroamines, opioid peptides, as they are influenced by genetics and environment.     

Regional brain [Met]-enkephalin in alcohol-preferring and non-alcohol-preferring inbred strains of mice

Scrutiny of the data from these studies reveals that the C58/J alcohol-preferring mice have significantly lower baseline methionine-enkephalin levels in both the corpus striatum and hypothalamus compared to C3H/CHRGL/2 non-alcohol-preferring mice. In other brain regions in these two strains, specifically, pituitary, amygdala, midbrain, and hippocampus, analysis of methionine-enkephalin levels did not show any significant differences. This suggests that the hypothalamus may indeed be a specific locus involved in the regulation of alcohol intake, via the molecular interaction between neuroamines, opioid peptides, as they are influenced by genetics and environment.     

Differences in teratogenic response and in capacity to repair in embryos of two inbred strains of mice

Zusammenfassung Die Wirkung des Farbstoffes Trypanblau auf die embryonale Entwicklung von 2 Mäusestämmen, des C57B1/6 Stammes und des Swiss-Albino-Stammes, wurde untersucht und die Ergebnisse wurden miteinander verglichen. Embryonen der beiden Stämme reagierten in verschiedener Weise auf Trypanblau. Die Injektion des Farbstoffes führte zum vollständigen Entwicklungsstillstand aller C57B1/6-Embryonen, während bei Swiss-Albino-Embryonen hauptsächlich Kopf-, Gehirn-und Schwanzabnormitäten auftraten. Bei Swiss-Embryonen nahm die Schwere der Abnormität mit fortschreitendem Alter der Embryos beträchtlich ab, obwohl die Anzahl pro Wurf unverändert blieb. Keine Veränderung in der Zahl der Abnormitäten wurde in älteren C57B1/6-Embryonen beobachtet. Die verschiedenartige Reaktion der beiden Stämme auf Trypanblau ist vielleicht darauf zurückzuführen, dass genetisch bedingte Unterschiede in der Fähigkeit, zerstörtes Gewebe während der embryonalen Entwicklung zu reparieren, vorhanden sind.

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Submitted in partial fulfillment of the requirements for the degree of Master of Arts in the Graduate Division of the College of Liberal Arts and Science of the City College of the City University of New York.     

Genetic differences between inbred strains of mice; a new source of variation in high-sulphur keratins

Summary Proteins were extracted by improved techniques from the hair of inbred strains of Peru, C57BL/6 and CBA/Ca mice. The extracts were characterized by amino acid analysis and high resolution polyacrylamide gel electrophoresis, and previously unreported strain differences were observed. Genetic analysis confirmed them.We are grateful to Dr Speakman, Dr J. Findlay and Prof. J. Shire for helpful discussion, the Joint Sequencing Unit (Dept of Biochemistry/Genetics) for carrying out the amino acid analyses, and the mouse house staff. We also thank the S.R.C. for the grant GR/B/62877 which supported this work.     

Genetic differences between inbred strains of mice; a new source of variation in high-sulphur keratins

Proteins were extracted by improved techniques from the hair of inbred strains of Peru, C57BL/6 and CBA/Ca mice. The extracts were characterized by amino acid analysis and high resolution polyacrylamide gel electrophoresis, and previously unreported strain differences were observed. Genetic analysis confirmed them.     

Different action of alpha-methyl-DOPA on sleep and labyrinth learning in 2 inbred strains of mice]

In two inbred mice strains C 57 BR and C 57 BL/6 presenting the same type of sleep, but a different capacity of learning, Alpha-Methyl-Dopa (100 mg/kg) injected after every session, suppresses paradoxical sleep completely for 9 to 11 h. Maze-learning performance is retarded in C 57 BR mice, but facilitated in C 57 BL/6.     

Regional and strain variation in brain 3′∶5′ cyclic adenosine monophosphate of inbred mice

Summary Regional variations were found in cAMP levels in flash frozen mouse brains with the pons and cerebellum having higher levels than the cerebrum. There were also strain variations with CBA/J and BALB/cJ having higher levels than C57B1/6J in the pons and cerebellum.     

Decidua and the control of corpus luteum function, follicular development and pituitary LHRH-responsiveness in pseudopregnant and pregnant rats

The mid-pregnancy rescue of corpora lutea can be mimicked in the pseudopregnant rat by induction of decidual tissue in the uterus: in such rats, around day 10, there is neither luteolysis, nor resumption of follicle-development or increase of the pituitary responsiveness to LHRH. The results suggest that the mid-pregnancy rescue of corpora lutea is caused by a maternal factor.     

Decidua and the control of corpus luteum function,follicular development and pituitary LHRH-responsiveness in pseudopregnant and pregnant rats

Summary The mid-pregnancy rescue of corpora lutea can be mimicked in the pseudopregnant rat by induction of decidual tissue in the uterus: in such rats, around day 10, there is neither luteolysis, nor resumption of follicle-development or increase of the pituitary responsiveness to LHRH. The results suggest that the mid-pregnancy rescue of corpora lutea is caused by a maternal factor.12 February 1987     

Regional and strain variation in brain 3':5' cyclic adenosine monophosphate of inbred mice.

Regional variations were found in cAMP levels in flash frozen mouse brains with the pons and cerebellum having higher levels than the cerebrum. There were also strain variations with CBA/J and BALB/cJ having higher levels than C57B1/6J in the pons and cerebellum.     

Suppression of intrinsic B-cell function in Dengue-infected mice.

Mice infected with Dengue virus show a depressed immune response to lipopolysaccharide (LPS), a helper T-cell-independent antigen, when LPS was administered on day 0, 6 and 12 post infection. Mice injected with inactivated virus failed to show immunosuppression.     

Renewal of noradrenaline in different zones of the central nervous system of inbred mice strains and their recombinants]

A good correlation exists between the learning capacity and norepinephrine metabolism in the neocortex of C57 and Balb inbred Mice strains, as well as their F1 hybrids and seven recombinant inbred strains derived from their cross. The animals with a better learning performance are characterised by low levels of norepinephrine, as well as a slow metabolic rate of this neurotransmitter in the cortex. Such a correlation has not been found to exist in the other cerebral regions studied.     

Cycloheximide-induced ultrastructural changes in the corpus luteum of rats

Résumé Le cycloheximide administré à des rats par voie i.v. modifie l'ultrastructure des cellules du corps jaune: ségrégation du nucléole, formation dans le cytoplasme de gros corps laminés et denses et agrégation des membranes lisses. On n'a pas encore établi si les modifications induites par le cycloheximide relèvent de l'inhibition de la synthèse des protéines ou d'une anomalie fonctionelle.

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Fellow of the Medical Research Council of Canada.     

Mutant expression in inbred lines and crosses

Zusammenfassung In Versuchen mit der Mutanteci ^D vonDrosophila melanogaster wurden die Komponenten der nichtgenetischen Varianz der Merkmalsausprägung bestimmt durch Einkreuzen vonci ^D in verschiedene Inzuchtstämme, Die unabhängige Komponente (V _i ) für die beiden Flügel ist mehrere Male grösser als die gemeinsame Komponente (V _c ). Nach Kreuzung zwischen den Inzuchtstämmen wurde keine Veränderung derV _i -Werte und in den Werten der Merkmalsausprägung keine Annäherung an den normalen Phänotypus festgestellt.