Erythrocyte catalase in liver cirrhosis and in experimental liver injury

Résumé Nous avons trouvé que l'activité de la catalase des erythrocytes chez les cirrhotiques et chez les chiens intoxiqués par CCl₄ est considérablement diminuée, tandis que le numéro globulaire et le taux d'hémoglobine ne représentent que des changements très peu signicatifs.     

Catecholamine content of the arterial walls in experimental hypertension

Zusammenfassung In Hunden mitGoldblatt-Hypertonie ist die Adrenalinkonzentration der Arterien stark erhöht, diejenige von Noradrenalin aber — verglichen mit den Werten der Kontrolltiere — erniedrigt. Die Adrenalinerhöhung in der Arterienwandung ist wahrscheinlich nicht die Ursache des Hochdruckes, da sie auch in Hunden ohne postoperative Blutdruckerhöhung gefunden wurde.     

The synthesis of total and specific glycosaminoglycans during development of experimental liver cirrhosis

Summary During hepatic fibrogenesis induced by long-term administration of thioacetamide, the synthsis of chondroitin 4,6-sulfate and hyaluronic acid was strongly enhanced; the formation of heparan sulfate comprising at least 70% of total liver GAG synthesis and of a keratan-sulfate-like fraction was stimulated 1.7fold. Formation of dermatan-sulfate in liver could not be detected.We are grateful to Prof. W. Kühnel, Department of Anatomy, and to Prof. R. Lindenfelser, Department of Pathology, RWTH Aachen, for critical examination of the histology.     

Centrioles in hepatocytes

Zusammenfassung In der normalen und regenerierenden Rattenleber wurden Zentriolen gefunden. In der normalen Leberparenchymzelle erscheinen sie wahrscheinlich als Diplosomen. Häufig werden mehr als 2 Zentriolen in der einzelnen Leberparenchymzelle beobachtet, jedoch gibt es keine Anhaltspunkte für eine zentrioläre Replikation in den normalen Leberparenchymzellen. Teilhepatektomie tritt kurz nach Bildung von Tochterzentriolen auf. Das Vorkommen von Zilien scheint mit den Zentriolen der Leberparenchymzellen nicht verbunden zu sein.

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This work was supported by grants to Prof.A. R. Currie from the Scottish Hospital Endowments Research Trust, and from the Medical Research Council for the purchase of the Siemens Elmiskop I. The technical assistance of Mr.R. Cardno is acknowledged.     

Isolated rat hepatocytes. Simultaneous study of variations in sialic acid content of glycoconjugated membranes and asialotransferrin uptake]

Hepatocytes isolated from streptozotocin treated Rats bind less asialotransferrin than hepatocytes isolated from normal rats. This decrease is parallel with a decrease in the sialic acid content. Insulin therapy restored simultaneously membrane sialic acid content and asialotransferrin binding capacity.     

Albumin synthesis in regenerating rat liver cells

Riassunto Gli autori hanno studiatoin vitro il problema della sintesi dell'albumina in sezioni di fegato normale e rigenerante di ratto. La quantità di albumina prodotta durante l'incubazione non presenta notevoli variazioni nelle due situazioni studiate. Al contrario l'incorporazione di glicina marcata nella albumina del fegato rigenerante risulta nettamente aumentata rispetto a quella del fegato normale. Tale comportamento viene interpretato come espressione di un ricambio albuminico più elevato nel fegato rigenerante che nel normale.

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This investigation was aided by a grant from C.N.R., Roma, and was made during a tenure by one of us (G. G.) of a Scholarship from C.N.R., Roma.     

Choline accumulation in isolated rat hepatocytes

Summary The accumulation of non-metabolized choline in isolated rat hepatocytes is concentrative in Na⁺ medium, whereas the accumulation does not exceed unity in a Li⁺ medium. Ouabain and 2,4-dinitrophenol inhibited the choline uptake. These results indicate that choline is taken up by rat hepatocytes via a Na⁺-and energy-dependent process, and choline oxidase is not directly connected with the choline transport system.     

Calcium content of experimental granuloma as determined by X-ray fluorescence. Influence of non-steroid anti-inflammatory drugs

Summary The deposit of calcium in experimental granulomas as determined by X-ray fluorescence is notably decreased by orally given indomethacin and diflunisal.     

Microinterferometric characterization of isolated human hepatocytes

Summary Hepatocytes isolated from liver tissue taken by biopsy from 18 patients with hepatic or extrahepatic diseases displayed a weight class-organization similar to that of other animal species. In most cases the cell classes had a period of 108 pg varying in number from 4 to 14; cell weight range was 96–432 pg as a minimum (4 classes) and 108–1536 pg as a maximum (14 classes). In 5 cases cell classes showed a period of 120 pg resulting 7–9 in number; cell weight range was 216–960 pg (7 classes) or 216–1200 pg (9 classes). No correlation was found between sex, age, liver histopathology, disease of the patients and the various parameters measured.This work was supported in part by a grant of the Consiglio Nazionale delle Ricerche, Rome, Italy.     

Autophagy in mouse hepatocytes induced by lysine acetylsalicylate

Summary I.v. administration of lysine acetylsalicylate inces autophagy in mouse liver cells. Single and multiple membrane-bounded vacuoles were found. The latter seems to be an unusual morphological form of the sequestration process. These findings could express a transitory sublethal liver cell injury induced by the drug.Acknowledgments. The authors are indebted to Mrs M. T. Correia and to Mrs M. E. Pereira for technical assistance.     

Lipid accumulation in mouse hepatocytes after morphine exposure

Summary After morphine injection lipid accumulation in mouse hepatocytes begins within 2 h and continues for 24 h when most hepatocytes are filled with lipid droplets. In spite of morphine maintenance the liver recovers as the accumulated lipids are coupled with protein and subsequently transported and released into the perisinusoidal space of Disse.Supported by USPHS Grants GM 15490, 5 SO7 RR05386-16 and DA-01310.     

DBP在难治性癫痫患者脑组织中的异常表达

目的通过观察白蛋白D．位点结合蛋白（DBP）和丝裂原活化蛋白激酶（MAPK）在难治性癫痫（IE）患者脑组织中的表达,探讨其在癫痫的发生、发展中的作用。方法用免疫组织化学及DBP／MAPK荧光双标法检测35倒难治性癫痫患者手术后脑组织标本中DBP、MAPK的表达,并与15例对照组进行比较。结果DBP主要在神经元表达,MAPK在神经元和胶质细胞中均有表达,主要分布于神经元,且IE中表达（DBP0．4737±0．0262、MAPK0．2935±0．0106）高于对照组（DBP0．2476±0．0229、MAPK0．2370±O．0125．P〈0．05）,荧光双标显示DBP与MAPK分布在同一神经元上。结论DBP和MAPK在难活性癫痫患者手术后脑组织标本中表达上调,且共同表达在同一细胞上,提示两者在难治性癫痫的发生发展中可能存在一定的相互作用。     

Effect of spironolactone on p-nitrophenol glucuronidation in isolated rat hepatocytes

The effect of spironolactone (SP) on p-nitrophenol (PNP) glucuronidation was studied in isolated rat hepatocytes with appropriate viability conditions. A significant increase of protein concentration and PNP glucuronidation was found in the hepatocytes from SP-treated rats. Increased enzyme activity apparently was related to the SP dose. The results favor the conclusion that SP may induce PNP glucuronidation in the hepatocyte.