东方百合杂交系部分栽培品种的遗传多样性分析

利用125条多态性随机引物对市场上具有代表性的10个东方百合杂交系栽培品种的遗传多样性进行了分析.结果表明：在125条引物中,30条引物能够扩增出多态性片段,引物多态性比例为24%.引物扩增片段数目从3到13条,其中S223可以完全区分10个供试品种.UPGMA法聚类结果表明,10个供试品种之间遗传距离为0.0930～05349,10个供试品种在相似系数0.57处可以分为3类,品种之间遗传变异比较丰富.其中Bernini和Tiber的遗传距离是0.0930,推测两者杂交亲本十分相近;而Aktiva与其它     

洞庭青鲫和洞庭青鲫(♀)×兴国红鲤()杂交F_1代的RAPD分析

以鱼的尾鳍为材料,采用苯酚—氯仿法提取鱼的基因组DNA.对提取的结果进行紫外分光光度、琼脂糖凝胶电泳方法的鉴定,筛选出浓度、纯度好的DNA样品进行进一步的RAPD扩增.采用上海生化工程有限公司的80个引物对洞庭青鲫和洞庭青鲫(♀)×兴国红鲤(♂)杂交F1代(简称“杂交鱼”)进行RAPD分析,筛选出9个引物对洞庭青鲫和杂交鱼进行了遗传多样性分析,这9个引物共检出洞庭青鲫89个位点、杂交鱼82个位点.其中多态性位点数:洞庭青鲫76个,杂交鱼71个分别占其总位点数的85.39%、86.58%.据RAPDdataanalyzer1.3v.exe数据分析程序计算,洞庭青鲫平均遗传相似度为0.6537;杂交鱼的平均遗传相似度为0.6218;两种群间的为0.5505.上述两项指标的初步分析表明,这两种鱼具有较为丰富的遗传多样性.     

洞庭青鲫和洞庭青鲫(?)×兴国红鲤(?)杂交F1代的RAPD分析

以鱼的尾鳍为材料,采用苯酚-氯仿法提取鱼的基因组DNA.对提取的结果进行紫外分光光度、琼脂糖凝胶电泳方法的鉴定,筛选出浓度、纯度好的DNA样品进行进一步的RAPD扩增.采用上海生化工程有限公司的80个引物对洞庭青鲫和洞庭青鲫(?)×兴国红鲤(?)杂交F1代(简称"杂交鱼")进行RAPD分析,筛选出9个引物对洞庭青鲫和杂交鱼进行了遗传多样性分析,这9个引物共检出洞庭青鲫89个位点、杂交鱼82个位点.其中多态性位点数:洞庭青鲫76个,杂交鱼71个分别占其总位点数的85.39%、86.58%.据RAPD data analyzer 1.3v.exe ＜RAPD data analyzer v1.3.xls>数据分析程序计算,洞庭青鲫平均遗传相似度为0.653 7;杂交鱼的平均遗传相似度为0.621 8;两种群间的为0.550 5.上述两项指标的初步分析表明,这两种鱼具有较为丰富的遗传多样性.     

皖西白鹅遗传多样性的RAPD分析

用随机扩增多态性ＤＮＡ（ＲＡＰＤ）技术对皖西白鹅产区内１１个不同地点的２４只个体一进行遗传多样性分析。从３４个随机引物中筛选出的１２个引物对所有个体的总基因组ＤＮＡ进行了ＰＣＲ扩增。共扩增出８７个ＤＮＡ片段,其中的２２个多态性片段,计算了各多态性片段在群体中分布的频率及各个体间的遗传距离指数。同时根据遗传距离指数对它们进行了聚类分析。作为家养动物的个品种,皖西白鹅的遗传多样性程度较高。     

内蒙古3个绒山羊品种RAPD的初步研究

利用随机扩增多态DNA（RAPD）技术,研究了内蒙古地区3个绒山羊品种的DNA多态性,由此推导它们之间的遗传距离和系统发育关系。在所使用的12种随机引物中,有8种随机引物扩增出多态谱带,共检测到32个RAPD标记,其中有21个发生变异。用Nei的片段公享度公式计算了品种间的遗传距离,用UPGMA聚类法构建了系统发育树。结果表明：内蒙古白绒山羊的3个类群彼此亲缘关系较近,分化不明显;内蒙古白绒山羊与罕山白绒山羊聚为一个大类,辽宁绒山羊和乌珠穆沁白绒山羊聚为一个大类,说明内蒙古白绒山羊与罕山白绒山羊有较近的亲缘关系,乌珠穆沁白绒山羊与辽宁绒山羊的亲缘关系较近。     

河南产香茶菜属6种植物的RAPD分析

从70个随机引物中筛选出10个具有较高多态性检测能力的引物,用于RA PD扩增,共扩增出85个条带,其中多态性条带59条,占68.81%.对得出的结果用SPSS12.0进行聚类分析,结果表明:河南产香茶菜不同种、同一种的栽培和野生之间存在明显的遗传分化,其中种间的差异要大于种内.从DNA分子水平上探讨了香茶菜属6种植物之间的遗传关系,为中药材冬凌草的品种鉴别提供依据.     

应用RAPD技术分析热带龙眼四季蜜与常规龙眼品种的遗传差异

为分析不同龙眼品种之间的遗传差异及亲缘关系,以近年从广西引进的四季蜜龙眼和原产广东、福建、广西的9个龙眼品种的叶片为材料,以基因组DNA为模版,使用100条随机引物进行RAPD扩增,其中18条引物扩增出清晰的具多态性的DNA谱带.采用UPGMA法对这18条引物扩增的谱带进行聚类分析发现,遗传距离系数为0.8时,可将10个龙眼品种(系)分为3组,第1组含四季蜜,第2组含灵龙,第3组含其余8个常规龙眼品种,它们是古山二号、储良、友谊、龙优、石硖、松风本、大乌圆和广眼.     

中国鹅5个品种随机扩增多态DNA(RAPD)分析

目的 用随机扩增多态ＤＮＡ（ＲＡＰＤ）技术分析中国鹅的５个品种一皖西白鹅、太湖鹅、浙江白鹅、四川白鹅和狮头鹅的随机扩增多态ＤＮＡ（ＲＡＰＤ）。方法 从３４个１０－寡核苷酸随机引物中筛选出的１４个引物对每一品种的总基因组ＤＮＡ进行了单引物ＰＣＲ扩增,结果 ５个品种共扩增出１７４个ＤＮＡ片段,其中４８个（占２７．６％）在５个品种间表现多态性,根据扩增ＤＮＡ片段的异同计算了各品种间的遗传距离指皖西白鹅和     

辣椒部分栽培种遗传相似性的RAPD分析

通过28个10bp的引物对30个辣椒栽培品种进行PCR扩增，共扩增出111条带，其中多态性条带有90条，占总条带数的81％，平均每个引物产生3．21个多态性条带，依据RAPD扩增结果，利用MEGA软件计算出各品种间的遗传相似性系数，构建Neighbor—Joining树形图，并结合辣椒品种的性状进行分析。结果发现：辣椒品种间的遗传相似性与辣椒果味或者果形之间总体上并无相关性，但在某些小组内，辣味品种内部或甜椒品种内部的遗传相似性高于这两种类型之间的遗传相似性；灯笼形辣椒和角形(羊角形和牛角形)辣椒品种内的遗传相似性也高于这两类品种之间的遗传相似性。试验结果为辣椒种质资源的鉴定以及育种提供了一定的参考依据。     

果蝇突变体的RAPD分析

采用RAPD技术对遗传学模式生物——果蝇的野生型及7种突变体的遗传差异进行比较研究．结果表明,所选用的37种RAPD引物中,有25种引物扩增谱带清晰,扩增总片断284条,单个引物的扩增片段数为6～15条,平均为11．36条．不同果蝇品种间的遗传差异较小,遗传距离（D）为0．0334～0．2089．根据D值,由UPGMA聚类分析绘制了它们的分子进化树。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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Brief Introduction to “Journal of Jilin University( Science Edition) ”

<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has