类胡萝卜素在光合细菌P4株中生物合成及紫外线防护

从光合细菌Ｐ４株提取的类胡萝卜素的主要成分为β－胡萝卜素，提取率约为０．３９％．菌体中类胡萝卜素的生物合成受环境中多种因素的影响．弱光、厌氧、较高温度有利于类胡萝卜素的生物合成，培养液中Ｆｅ３＋离子浓度在５ｍｇ／Ｌ左右对其合成有明显的促进作用，碳源、氮源对类胡萝卜的生物合成影响不大，但对菌体生长速率有明显影响，从而也影响到获得类胡萝卜素的绝对量．从Ｐ４菌体中提取的类胡萝卜素对真核细胞有良好紫外线防护作用，当培养液中其含量仅为０．２μｇ／Ｌ时，保护率接近６０％．     

红酵母sp.8生物合成类胡萝卜素的研究

研究了红酵母（Ｒｈｏｄｏｔｏｒｕｌａ ｓｐ．８）利用霉菌的玉米粉水解液合成类胡萝卜素的过程。结果表明，黑曲霉ＡＱ７２的玉米粉发酵液适于红酵母Ｒ．ｓｐ 的生长及类胡萝卜素的合成。在６０ ｇ／Ｌ玉米粉为底物，经黑曲霉水解后，摇瓶震荡培养红酵母８４ ｈ，生物量干细胞达１３．４ ｇ／Ｌ，色素含量为３．４２ ｍ ｇ／Ｌ。经高效液相色谱测定，其色素中，含量最高的是β－胡萝卜素，达２．６７ ｍ ｇ／Ｌ。该研究为生物合成天然色素代替人们忌讳的化学合成色素提供了新的途径     

光合细菌类胡萝卜素发酵的研究

首先，优化并确定了光合细菌培养基配方：以乳酸钠为碳源，谷氨酸钠为氮源，用酵母膏来替代微量元素和生长因子。在此基础上进一步确定了最佳发酵工艺条件：ｐＨ值７．０，温度３０ ℃，光照度８００ ｌｘ，微好氧培养，接种量１０％ 。在此条件下，经发酵试验取得较好的结果为：菌体干重为４．２ｇ／Ｌ，类胡萝卜素得率为２６．５ ｍ ｇ／Ｌ。     

整函数与基样条的关系

主要结果是：对ｆ∈Ｌ２（Ｒ），有ｌｉｍＥ（ｆ，π／σ，ｍ∩Ｌ２（Ｒ））２（Ｒ）＝Ｅ（ｆ，σ∩Ｌ２（Ｒ））２（Ｒ），且存在ｆ０∈Ｌ∞（Ｒ），使得ｌｉｎＥ（ｆ０，π／σ，ｍ∩Ｌ∞（Ｒ））∞（Ｒ）＞Ｅ（ｆ０，σ∩Ｌ∞（Ｒ））∞（Ｒ），其中π／σ，ｍ为ｍ－１次的以｛（１／２）ｊπσ－１（ｍ＋１）｝ｊ∈Ｚ为节点的基样条的全体，σ表示指数σ型的整函数在Ｒ的限制的全体，其中σ＞０。     

应用均匀设计方法改进光合细菌类胡萝卜素生产

应用均匀设计的方法，对光合细菌球形红杆菌７－１菌株的类胡萝卜素生产的培养基组成进行优化，得到的优化条件为：乳酸钠１．２％；谷氨酸钠０．７％；硫酸镁１００ｍｇ／Ｌ；硫酸铁１５ｍｇ／Ｌ；酵母膏０．２％，在此条件下，类胡萝卜素产量比优化前提高６１％．     

甘蓝型油菜萝卜质不育系恢复材料Ad-6(F4)测交二代恢复株TCF2的快繁及生根

油菜萝卜胞质不育系恢复材料Ａｄ－６（Ｆ４）测交二代恢复株ＴＣＦ２,在ＭＳ附加５ｍ ｇ／Ｌ６－ＢＡ＋ ０．５ｍ ｇ／Ｌ ＮＡＡ,３ｍ ｇ／Ｌ ６－ＢＡ＋ ０．２ｍ ｇ／Ｌ ＮＡＡ,３ｍ ｇ／Ｌ ６－ＢＡ＋ ０．３ｍ ｇ／Ｌ ＮＡＡ＋６００ｍ ｇ／Ｌ水解乳蛋白以及５ｍ ｇ／Ｌ６－ＢＡ＋ ０．３ｍ ｇ／ＬＮＡＡ＋ ５ｍ ｇ／ＬＡｇＮＯ３ 的不同培养基上诱导丛生芽,结果表明５ｍ ｇ／Ｌ６－ＢＡ＋ ０．３ｍ ｇ／ＬＮＡＡ＋ ５ｍ ｇ／ＬＡｇＮＯ３ 对ＴＣＦ２ 诱导丛生芽有较好的效果．小芽生根培养基为１／２ＭＳ效果最好．     

光合细菌中类胡萝卜素对脂质过氧化抑制作用研究

光合细菌中的类胡萝卜素有许多不同类型,从Ｒｄ．ｓｐｈａｅｒｏｉｄｅｓＰ４株中提取的类胡萝卜素主要是β－胡萝卜素,而从Ｒｄ．ｃａｐｓｕｌａｔａＮ３株中提取的主要是蕃茄红素．在两个重要离体系统中（即Ｆｅ２＋－牛血清蛋白－亚麻油酸和Ｆｅ２＋－脑组织匀浆）,这些类胡萝卜素具有显著的抗脂质过氧化作用,当系统中类胡萝卜素浓度为０．２ｍｇ／Ｌ时,它们对脂质过氧化抑制率接近或超过８０％．进一步研究表明,它们能明显地抑制邻苯三酚的自氧化速率,这证明它们对超氧阴离子也具有很好的淬灭作用．     

马铃薯酪氨酸酶的部分纯化及理化性质的研究

马铃薯酪氨酸经粗提、丙酮沉淀和酸铵分级后，得到了纯化１．８倍，活力回收４２．５％，比活力为４９．７Ｕ／ｍｇ蛋白的部分纯化酶，并对其理化性质进行了研究。结果表明，以多巴为底物，酶的Ｋｍ为５．７ｍｍｏｌ／Ｌ，Ｖｍ为１６．２μｍｏｌ／Ｌ?ｍｉｎ，最适ＰＨ为８．２，最适温度为４０℃，ＨＳＯ＾－３Ｆ＾－、苯甲酸、叠氮化钠、过氧化氢为其抑制剂，Ｖｃ、苯肼等对该酶的半抑制浓度为μｍｏｌ／Ｌ水平。Ｃｕ＾２＋在低浓     

类胡萝卜素产生菌紫色非硫菌1S—10的培养优化条件研究

对一株类胡萝卜素产生菌紫色非硫菌１Ｓ－１０的培养条件进行了优化。结果表明：培养基中的碳源、氮源、培养温度、光照度等对该菌生物量和类胡萝卜素含量均有影响。在确定的优化条件下，细胞生物量为２．８７ｇ／Ｌ培养基，类胡萝卜素含量为４８．５６ｍｇ／ｇ干菌体。     

层状金属氧化物的离子交换性能研究

本文利用ＸＲＤ，ＳＥＭ，ＤＴＡ及ＢＥＴ等现代仪器，研究了Ｋ－Ｚｎ－Ｔｉ层状金属氧化物（ＬＭＯ）经ＨＡＣ，ＮＨ４Ｃｌ交换前后的基本性能。结果表明：该ＬＭＯ具有良好的离子交换性能，其面网间距由０．７８ｎｍ分别增加到０．８６ｎｍ和０．８８ｎｍ．同时，比表面积由２７ｍ２／ｇ分别增加到３０ｍ２／ｇ和５９ｍ２／ｇ，可望作为大孔分子筛的制备原料。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

正~~