丛柳珊瑚Euplexaura rhipidalis中细胞毒活性二萜成分分析

为了寻找结构新颖的海洋活性成分,对一种采自中国西沙海域丛柳珊瑚Euplexaura rhipidalis进行分离和纯化,并对获得的西松烷二菇化合物的抗肿瘤活性进行筛选,以探明该种丛柳珊瑚的活性组成成分.先利用正反相硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备HPLC等方法进行分离和纯化,再利用NMR、MS等波谱方法,并与文献对照确定了3个高氧化度的西松烷二萜类化合物,分别为eorulloidolide A（1）,（1R,3S,4S,7E,11E）-3,4-epoxycembra-7,11,15-triene（2）,（-）-corulloidolide E（3）;细胞毒活性模型评价结果发现,化合物3表现了较强的细胞毒活性,对5种癌细胞肝癌HepG2,Hep3B,乳腺癌阿霉素耐药株MCF-7/ADR,前列腺癌PC-3he,结肠癌HT-116的IC50值都低于10 μg/mL.表明西松烷二萜类化合物是该丛柳珊瑚E.rhipidalis的主要活性化合物类型之一.     

刺五加根茎活性成分对蛋白酪氨酸磷酸酶1B的抑制作用

采用正相硅胶柱色谱、 C-18反相柱色谱和半制备型高效液相色谱（HPLC）等多种层析方法分离、 纯化刺五加根茎乙酸乙酯层中的降糖活性成分, 得到10个化合物, 通过核磁共振氢谱(¹H-NMR)、 核磁共振碳谱（¹³C-NMR）、 无畸变极化转换技术(DEPT)、 质谱（MS）等方法分析各化合物的光谱数据, 并与相关文献比较, 确定所得化合物的结构, 对其进行活性测试. 结果表明： 化合物1~5为贝壳杉烷型二萜类化合物, 化合物6~8为松脂烷型二萜类化合物, 化合物9,10为木脂素类化合物; 化合物1,5,7,8对蛋白酪氨酸磷酸酶1B(PTP1B)具有明显的特异性抑制活性, 化合物2,4,9对蛋白磷酸酶（PP1）具有较好的抑制活性,  化合物9,10对牛痘H1相关磷酸酶（VHR）的抑制活性作用较低.     

民族药骚羊古化学成分的研究

用硅胶柱色谱等方法,从贵州省民族药骚羊古(Pimoinella candolleana Weight et Arn.)全草中分离出5个化合物,根据化合物的一维、二维核磁共振波谱等解析,分别鉴定为三十二烷(Ⅰ)、β-谷甾醇(Ⅱ)、豆甾醇(Ⅲ)、熊果酸(Ⅳ)和1-羟基-2,3,5-三甲氧基(口山)酮(Ⅴ),化合物Ⅰ～Ⅴ均首次从该植物中分得.此外,还对化合物Ⅴ的核磁共振二维谱进行了解析,确定的各个1H和13C的归宿.     

四数九里香的化学成分研究

采用硅胶柱层析色谱和重结晶方法对四数九里香(Murraya tetrameraHuang in Acta Phytotax.Sin.)的非挥发油成分进行分离和纯化,通过理化性质和波谱技术鉴定化合物结构。结果分离并鉴定出5个化合物:补骨脂素、槲皮素3-O-β-D-吡喃葡萄糖苷、咖啡酸、正三十一烷、正三十四烷醇。5个化合物均为首次从该植物中分离得到。     

民族药骚羊古化学成分的研究

用硅胶柱色谱等方法,从贵州省民族药骚羊古(PimoinellacandolleanaWeightetArn )全草中分离出5个化合物,根据化合物的一维、二维核磁共振波谱等解析,分别鉴定为三十二烷(Ⅰ)、β-谷甾醇(Ⅱ)、豆甾醇(Ⅲ)、熊果酸(Ⅳ)和1-羟基-2,3,5-三甲氧基酮(Ⅴ),化合物Ⅰ～Ⅴ均首次从该植物中分得。此外,还对化合物Ⅴ的核磁共振二维谱进行了解析,确定的各个1H和13C的归宿。     

东革阿里树皮的化学成分研究

利用溶剂提取和正、反相色谱法、Sephadex LH-20凝胶柱色谱等技术对泰国产东革阿里(Eurycoma longifolia)树皮的化学成分进行分离纯化.采用核磁共振、质谱等波谱技术鉴定化合物的结构.从泰国产东革阿里树皮提取物中分离得到8个化合物,分别鉴定为铁屎米酮(1),铁屎米酮-N(3)-氧化物(2),9-羟基铁屎米酮(3),9-甲氧基铁屎米酮(4),9,10-二甲氧基铁屎米酮(5),东哥内酯(6),宽缨酮(7),豆甾-4,22-二烯-3-酮(8).化合物1~5为β-咔巴啉类生物碱,化合物6~7为苦木萜类成分,化合物8为甾体化合物,除化合物5和6外,其他6个化合物均为首次从该植物树皮中分离得到.     

还阳参化学成分的分离及结构鉴定

研究还阳参Crepis tectorum Linn.根的化学成分。采用柱层析、高效液相色谱等方法进行分离和纯化,通过理化、核磁共振等现代波谱技术鉴定化合物结构。从还阳参根石油醚部分分离得到3个化合物,分别鉴定为二十六烷醇(Ⅰ)、β-谷甾醇(Ⅱ)、蒲公英醋酸酯(Ⅲ);从其乙酸乙酯部分分离得到2个化合物,鉴定为咖啡酸(Ⅳ)和伪蒲公英甾醇乙酸酯(Ⅴ)。     

丹参(Salvia miltiorrhiza)中一对具有新奇结构的立体异构体的结构测定

为了进一步弄清丹参的化学成分，应用化学和色谱分离方法从丹参中分离到一个白色粉末固体，经综合运用先进的波谱分析手段，包括１Ｈ－１ＨＣＯＳＹ、１Ｈ－１３ＣＣＯＳＹ、ＣＯＬＯＣ、ＮＯＥ等二维核磁共振谱确定了它们的化学结构为一对具有螺环缩酮内酯结构的新二萜类立体异构体化合物．     

民族药骚羊古化学成分的研究

用硅胶柱色谱等方法，从贵州省民族药骚羊古(Pimoinella candolleana Weight et Arn.)全草中分离出5个化合物，根据化合物的一维、二维核磁共振波谱等解析，分别鉴定为三十二烷(Ⅰ)、β-谷甾醇(Ⅱ)、豆甾醇(Ⅲ)、熊果酸(Ⅳ)和1-羟基-2，3，5-三甲氧基咄酮(Ⅴ)，化合物Ⅰ～Ⅴ均首次从该植物中分得。此外，还对化合物V的核磁共振二维谱进行了解析，确定的各个^1H和^13C的归宿。     

忧遁草的化学成分研究

采用溶剂萃取、硅胶色谱柱、C18反相色谱柱、LH-20凝胶色谱柱、高效液相色谱等方法分离纯化,通过核磁共振波谱和质谱及旋光仪等仪器鉴定忧遁草化合物的结构.从忧遁草的95%乙醇提取液中分离鉴定9个化合物,分别鉴定为黑麦草内酯(1),异黑麦草内酯(2),3-羟基-9甲氧基紫檀烷(3),蚱蜢酮(4),(-)evofolin B(5),3-羟基-1-(4-羟基-3,5-二甲氧基苯)-1-丙酮(6),吲唑(7),吲哚-3-甲醛(8),entadamide A(9).其中化合物3-6,9为首次从忧遁草中分离得到.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that