Studies on the reaction between Laccase ando-methoxyphenol by microcalorimetry

The reaction between Laccase ando-methoxyphenol have been studied by LKB-2107 batch microcalorimetry system. Thermodynamic parameters Δ _r H _m , ΔG _o , ΔG _T ^≠ and kinetic parameters (K _m ,k ₂) have been determined. The process of the reaction has been analyzed from changes in energy by using the transition state theory. Two methods for enhancing catalytic power of Laccase are proposed. The results shown that formation of an enzyme-substrate complex is “anticatalytic”. The enter and sole source of catalytic power is the stabilization of transition state; reactant-state interactions are by nature inhibitory and only waste catalytic power. Supported by the National Natural Science Foundation of China Wang Tianzhi: born in 1968. Ph D     

漆酶与各种类型底物反应性能的微量热法研究

在厌氧条件下,用ＬＫＢ－２１０７Ｂａｔｃｈ微量热系统研究了在２９８．１５Ｋ０．１ｍｏｌ／Ｌ磷酸盐缓冲溶液（ｐＨ７．４）中漆树漆酶与５种底物的反应,测定了各反应的反应焓变△Ｈｍ米氏常数Ｋｍ,酶底复合过程的吉布斯自由能△Ｇ０复合物中电子转移速率常数ｋ２,以及漆病对各种底物的催化活性ＥＡ．讨论了相同的实验条件下,各种酶底复合物的稳定性,底物官能团及其位置对酶反应中的电子转移速率和非酶反应中自由基聚合形式     

Effect of surfactant on the catalyzed oxidation of 2,6-dimethylphenol by rhus vernicifera laccase

Rhus vernicifera laccase-catalyzed oxidation of 2,6-dimethylphenol (DMP) was studied in the presence of 12 surfactant (Sf). C₁₆H₃₃N⁺ (CH₃)₃ Br⁻ (CTAB) etc 5 cationic Sf and C₁₂H₂₃SO₄Na (SDS) etc 3 anionic Sf can promote the reaction in the different extent. The promotion of CTAB is the largest among them. The affected factors of the reaction—concentration of CTAB and DMP, pH, temperature, laccase quantity and reaction kinetics are inverstigated further. The nonionic Sf-Tween-20, Tween-60, Tween-80 and Triton X-100 have not promotion to the reaction. Ji Licai: born in 1945, Associate professor     

生命金属离子对漆酶催化活性的影响

以５,６二氯２,３二氰基氢醌为底物,在ｐＨ＝４．４０条件下,用分光光度法考察了１１种生命金属离子（Ｎａ＋、Ｋ＋、Ｃａ２＋、Ｍｇ２＋、Ｍｎ２＋、Ｆｅ２＋、Ｃｏ２＋、Ｃｕ２＋、Ｃｒ６＋、Ｎｉ２＋、Ｚｎ２＋）存在下的漆酶催化氧化反应,发现Ｆｅ２＋离子对漆酶的催化活性显示出抑制作用,探讨了其抑制特征、抑制位点和作用方式．结果表明,Ｆｅ２＋离子对漆酶催化活性抑制属竞争性抑制过程,Ｆｅ２＋离子的抑制作用是通过还原漆酶反应产物半醌自由基阴离子来完成的．Ｆｅ２＋离子漆酶复合物的解离常数（Ｋｉ）经测定为１７．５×１０－６ｍｏｌ·Ｌ－１．     

漆树漆酶的催化氧化作用Ⅺ原儿茶酸酯类的漆酶酶催氧化

用分光光度法对原儿茶酸甲酯、正丁酯、正辛酯底物在不同ｐＨ条件下的离解和漆酶催化氧化反应进行了考察，测定了它们在ｐＨ＝８．０时的反应速度常数．结果表明，不为漆酶所喜爱的亲水性底物（原儿茶酸），经过适当疏水化处理，可以有效地改善其反应性和提高漆酶的催化活性．ＥＳＲ结果证明，这些底物的漆酶催化反应都经历了半醌自由基阴离子阶段；酯基的存在不能保证底物顺利地氧化成相应邻醌，用二苯乙二胺只能捕获到少量羟基化的邻醌     

Studies on reduced extent method for inhibited thermokinetics of enzyme-catalyzed reaction

The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced, and then the criteria for distinguishing inhibition type was given and the methods for calculating kinetic parameters,K _M,K _i andv _m were suggested. This theory was applied to inverstigate the inhibited thermokinetics of laccase-catalyzed oxidation ofo-dihydroxybenzene bym-dihydroxybenzene. The experimental results show the inhibition belongs to reversible competitive type,K _M=6.224×10⁻³ mol·L⁻¹,K _i=2.363×10⁻² mol·L⁻¹. Xiong Ya: born Sep. 1961, Ph. D. graduate student. Curent research interest is in biothermochemistry research Supported by the National Natural Science Foundation of China     

Study on the action of sodium selenite on the mitochondria metabolism of<Emphasis Type="Italic">Carassius auratus</Emphasis> hybrid carps liver by microcalorimetry

By using an LKB-2277 bioactivity monitor and ampoule method, the fundamental thermogenesis curves of the metabolic process of liver mitochondria fromCarassius auratus hybrid carps and the toxic effect of Na₂SeO₃ on it were studied at 28°C. From the thermogenesis curves, the thermokinetic equations were established under different condition. The kinetics show that Na₂SeO₃ has toxic action on the metabolism process ofCarassius auratus hybrid carps liver mitochondria. Foundation item: Supported by the National Science Foundation of China (29973030, 30170010 and 5020310), Chenguang Project of Wuhan and National Postdoctor Foundation of China Biography: Li Xi (1968-), female, Associate pofessor, research direction: biothermochemistry and biomaterials.     

Studies inin situ PCR detected by the BrdU antibody technique

Using the BrdU antibody technique followed by an immuno-chemical staining (BAT), the amplification of DNA fragments specific to human Y chromosome on cell specimen slides was efficiently detected. Whether direct BrdU incorporation into PCR products orin situ hybridization with PCR products on slides, the amplified target DNA fragments of specimen were visualized by BAT under the microscope. The availability of BAT and differences in the sensitivity and efficiency between BAT and dig-11-dUTP labeling in cellin situ PCR were discussed. Zhang Xiyuan: born in Oct. 1935, Professor, Current research interest in Cellular and Molecular Biology Supported by the National Natural Science Foundation of China     

Microcalorimetric study on the antibiotic activity of clarithromycin and erythromycin

By using LKB-2277 Bioactivity Monitoring System, the heat effect changes in the process of inhibitory action of clarithromycin and erythromycin onEscherichia coli at 37°C were determined. Quantitative analysis showed that relationship between antibiotic concentrationc and rate contantk ofEscherichia coli growth, and half inhibitory ratio concentration IC₅₀: clarithromycin:k=0. 030 03–1. 1736×10⁻³ c, 8. 45 mg ·L⁻¹; erythromycin:k=0.031 08–8.4657×10⁻⁴ c, 14. 45 mg·L⁻¹. As a result of the microcalorimetry experiments, it not only indicated that antibacterial activity of clarithromycin was stronger than that of erythromycin, but also reported the changeable features of thermodynamics of the bacterial cell in biological, biochemical and metabolic process under different drug action. Foundation item: Supported by Natinal Natural Science Fundation of China (29973030), Natural Science Fundation of Hubei Province (98J052) and Post-doctoral Science Fundation of China Biography, SHEN Xue-song (1956-), Associate professor Research direction: biothermochemistry.     

用GC法研究叔丁基氯的水解反应

研究用ＧＣ测定叔丁基氯水解反应的方法。该测定方法不需分离提纯，而是根据混合物中反应物叔丁基氯和生成物叔丁醇的不同保留时间以及不同峰面积，直接从ＧＣ图上就可算出ｔ－ＢｕＣｌ和ＢｕＯＨ的相对含量，进一步计算其反应速度常数和活化能。该测定方法操作简便，重现性好。     

Electrochemical redox of hemoglobin at poly(o-phenylenediamine) film modified electrodes or promoted byo-phenylenediamine

Electrochemical redox of hemoglobin at poly(o-phenylenediamine) coated glass carbon electrodes or at bare glass carbon electrodes promoted byo-phenylenediamine have been investigated. The electrochemical characters of poly(o-phenylenediamine) films have also been studied by using cyclic voltammetry and potential-step chronoamperometry as well as current-step chronocoulometry techniques. A possible catalyzing mechanism for hemoglobin is proposed. Supported by the National Natural Science Foundation of China Ye Baoxian Born in July 1958. Ph. D., Associate professor, precent communication address is College of Chemistry and Chemical Engineering, Zhengzhou University, Zhengzhou, 450052     

Effects of ubiquitin-proteasome pathway on mouse sperm capacitation, acrosome reaction and in vitro fertilization

Chlortetracycline (CTC) fluorescence patterns were used to study changes in the patterns B and AR of mouse sperm after incubation with reagents that would block the UPP. They were the monoclonal antibody against ubiquitinated proteins—UCP1; the polyclonal antibody against ubiquitin—anti-Ub, and a special inhibitor against proteasome—ALLN. Furthermore, we treated the capacitated sperm or the eggs with these reagents separately and tested whether the normal in vitro fertilization was blocked or not. Results illustrate that UCP1, anti-Ub, and ALLN have little effects on sperm capacitation and acrosome reaction, but they do inhibit fusion of mouse sperm with eggs, which suggests that UPP play an important role in mouse in vitro fertilization.     

丙二酸与光活性脂环酮的成环反应研究

在乙酸酐和少量硫酸存在下，丙二酸与具光学活性脂环酮反应缩合成Ｍｅｌｄｒｕｍ酸的类似物．除（－）－薄荷酮生成相应产物产率可达３２％外，（Ｒ）－（十）－樟脑、（Ｓ）－（－）－樟脑和（Ｒ）－葑酮的反应产率仅有１～２％．（Ｒ）－诺蒎酮在这反应条件下发生重排反应，生成丙二酸（４’－亚异两基）亚环己酯，产率为３３％．     

Characterization of type<Emphasis Type="Italic">p</Emphasis> Banach spaces by the weak law of large numbers

For weighted sums of the form where {a _nj , 1 ⩽j⩽k _n ↑∞,n⩾1} is a real constant array and {X _aj , 1≤j≤k _{n, n}≥1} is a rowwise independent, zero mean, random element array in a real separable Banach space of typep, we establishL ^r convergence theorem and a general weak law of large numbers respectively, conversely, we characterize Banach spaces of typep in terms of convergence inr-th mean and probability for such weighted sums. Foundation item: Supported by the National Natural Science Foundation of China (No. 10071058) Biography: Gan Shi-xin (1939-), male, Professor, research direction: martingale theory, probability limiting theory and Banach space geometry theory.     

Studies on a series complexes of copper (II), zinc (II), cobalt (III) with Schiff base derived fromd-glucosamine and salicylaldehyde

Copper (II) zinc (II) cobalt (III) complexes with the Schiff base derived from salicylaldehyde andd-glucosamine were synthesized. These compounds abbreviated as SG, CuSG, ZnSG, CoSG were characterized by elemental analyses, IR, UV-Vis,¹H NMR and MS spectrum. ESR spectrum, magnetic susceptibility measured by Evans method for CuSG had also been done. It is suggested that the complexes in solution have pseudotetrahedral structure. It was found that SG would occur a photochemical reaction if a beach of 355 nm ultravisible light shone on its aqueous solution. The reaction was in further research. Supported by the National Natural Science Foundation of China Ye Yong: born in 1970, Ph. D graduate student     

葛根总黄酮振荡行为的微量量热法研究

采用微量量热法对中药活性成分葛根总黄酮振荡体系进行了研究,测定了葛根总黄酮在不同浓度、不同温度下的振荡体系的热功率-时间曲线,得到了它的诱导期、振荡周期,根据动力学理论计算了它的反应级数和表观活化能,并建立了诱导期、振荡周期与浓度和表观活化能之间的非线性关系式．     

Synthesis of layered LiMnO2 byin situ oxidation-intercalation and a study of the reaction mechanism and electrochemical performance

Using Mn(OH)₂ as precursor, LiOH as lithiating agent and (NH₄)₂S₂O₈ as oxidant, layeredo-LiMnO₂ was obtained by a novel method—in situ oxidation-intercalation under mild conditions (80 °C). The product was characterized by XRD, ICP, TEM and⁷Li-NMR. The results reveal that orthorhombic LiMnO₂ with high purity and good crystallinity can be obtained by this method. During electrochemical tests, a LiMnO₂/Li cell shows an initial reversible capacity of 208 mAh · g⁻¹ and a reversible capacity of 180 mAh · g⁻¹ after 30 cycles at room temperature.     

Two-parameter vector-valued martingales and geometrical properties of Banach spaces

We obtained a number of inequalities and laws of large numbers for two-parameter vector-valued martingales. In the other direction we characterizedp-smoothness andq-convexity of Banach spaces by using these inequalities and laws of large numbers for two-parameter vector-valued martingales. Biography: CHENG Ri-yan (1968-), male, Master, now works in Department of Mathematics, Fujian Normal University, Fuzhou 350007, China, His current research interest is in random processes.