大足蝠八种组织乳酸脱氢酶（LDH）同工酶比较研究

本文用聚丙烯酰胺不连续凝胶盘电泳,对大足蝠8种组织的LDH同工酶进行了报道。大足蝠心肌、肾、脑、血清、睾丸、胸肌、大腿肌组织的LDH同工酶的5条主带,以H亚基的活性最高,肝组织的5条主带以M亚基的活性最高。值得注意的是,在血清的谱带中,在LDH_3和LDH_4之间,出现了一条额外的LDH_e带,在心肌、肝LDH中,还分别出现了一条向阳极泳动比LDH_1还快的带,暂称为LDH_0带。     

藏系绵羊心肌、骨骼肌MDH和LDH活力及LDH同工酶谱

为了探索草地藏系绵羊高原适应性的分子机制,对草地藏系绵羊骨骼肌、心肌中乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)活力进行测定,并采用聚丙烯酰胺凝胶电泳分析了其心肌、骨骼肌、肝脏中LDH同工酶谱.结果显示:藏系绵羊心肌MDH/LDH活力比值极显著高于骨骼肌(P<0.01,LDH同工酶的表达在心肌、骨骼肌、肝脏中表现出组织特异性,LDH在肌肉中总活力最高,在电泳凝胶上表现为5条酶带,其中LDH5相对活力最强.     

兴国红鲤成体组织中四种同工酶的研究

采用聚丙烯酰胺凝胶垂直平板和盘状电泳，分析了兴国红鲤的脑、肝、肌、心、肾、眼六种新鲜组织及血清中的乳酸脱氢酶（LDH）、苹果酸脱氢酶（MDH）、酯酶（EST）及葡萄糖－6磷酸脱氢酶（G6PD）等四种同工酶系统的酶带及其血清蛋白带。结果表明，不同组织中四种同工酶谱带是不相同的，存在着明显的组织特异性。血清蛋白分析显示雌雄鱼体中蛋白带有显著差别。     

酵母两种形态菌体的MDH和SOD同工酶的电泳分析

用不连续ＰＡＧＥ对酵母两种形态菌体中ＭＤＨ同工酶（苹果酸脱氢酶）和ＳＯＤ同工酶（超氧化物歧化酶）进行电泳分析,发现在ＧＹＲ中培养１２ｈ时（多细胞占主体）的ＭＤＨ同工酶酶带比ＧＹＰ中的多３条;ＳＯＤ同工酶酶带则多４条。     

铁胁迫对大豆POD和EST同工酶的影响

采用垂直聚丙烯酰胺凝胶电泳,研究了2个品种的大豆--浙春3号和1601在不同时期、不同浓度铁胁迫下过氧化物酶(POD)同工酶和酯酶(EST)同工酶的变化情况.结果表明,浙春3号的2种同工酶酶谱的变化基本一致,随铁浓度的增加酶带数和强度都有所增加;而1601的2种同工酶,在高浓度铁处理时,酶带数和强度都减少.2个大豆品种不同时期的POD和EST同工酶酶谱对铁胁迫的反应也有差异.不同品种大豆的同工酶酶谱的变化,与其对铁胁迫的抗性有关.     

人工养殖下棘胸蛙的LDH同工酶冬眠前后的变化

利用聚丙烯酰胺垂直板电泳法,研究了平原地区人工养殖的棘胸蛙在冬眠前、中、后三个期间中,肝、肾、心、肌四种组织的乳酸脱氢酶酶谱,发现乳酸脱氢酶同工酶酶谱在不同组织中有差异,且在冬眠前后有一定的变化;初步探讨了季节对LDH同工酶的影响。     

贵州5种蝾螈几种组织中乳酸脱氢酶同工酶(LDH)的电泳分析

采用聚丙烯酰胺垂直板凝胶电泳技术 ,分析了贵州疣螈 ,细痣疣螈 ,无斑肥螈 ,蓝尾蝾螈和尾斑瘰螈若干种组织的LDH同工酶 ,结果表明 5种蝾螈种属差异明显 :贵州疣螈 ,细痣疣螈的LDH为 5谱带型 ,而无班肥螈 ,蓝尾蝾螈和尾斑瘰螈的LDH则为非五谱带型 ,各种的LDH亚基构成和迁移率均有所不同。依据 5个种的LDH同工酶的种属差异 ,对它们的系统关系进行了讨论。     

Population status, threats and conservation of the Yangtze finless porpoise

The Yangtze finless porpoise （Neophocaena phocaenoides asiaeorientalis） is currently limited to the middle and lower reaches of the Yangtze River from Yichang to Shanghai, China, and the adjoining Poyang and Dongting Lakes. Its population size has decreased remarkably during the last several decades due to the heavy impact of human activities, including overfishing of prey species, water development projects that cause attendant habitat loss and degradation, water pollution, and accidental deaths caused by harmful fishing gear and collisions with motorized vessels. It was estimated that the number of remaining individuals was down to approximately 1800 in 2006, a number that is decreasing at a rate as high as 5% per year. Three conservation measures - in situ and ex situ conservation and captive breeding have been applied to the protection of this unique porpoise since the early 1990s. Seven natural and two ＂semi-natural＂ reserves have so far been established. Since 1996, a small group of finless porpoises has been successfully reared in a facility at the Institute of Hydrobiology of the Chinese Academy of Sciences; three babies were born in captivity on July 5, 2005, June 2, 2007 and July 5, 2008. These are the first freshwater cetaceans ever born in captivity in the world. Several groups of these porpoises caught in the main stream of the Yangtze River, or rescued, have been introduced into the Tian＇e-Zhou Semi-natural Reserve since 1990. These efforts have proven that, not only can these animals survive in the area, they are also to reproduce naturally and successfully. More than 30 calves had been born in the reserve since then, with one to three born each year. Taking deaths and transfers into account, there were approximately 30 individuals living in the reserve as of the end of 2007. Among eight mature females captured in April 2008, five were confirmed pregnant. This effort represents the first successful attempt at off-site protection of a cetacean species in the world, and establishes a solid base for conservation of the Yangtze finless porpoise. A lesson must be drawn from the tragedy of Chinese River Dolphin （Lipotes vexillifer）, which has already been declared likely extinct. Strong, effective and appropriate protective measures must be carried out quickly to prevent the Yangtze finless porpoise from becoming a second Chinese River Dolphin, and save the biodiversity of the Yangtze River as a whole.     

大银鱼Protosalanx hyalocranius(Abbott)不同组织LDH同工酶、EST同工酶的初步研究

报道了应用聚丙烯酰胺凝胶垂直板电泳(PAGE)研究大银鱼Protosalanxhyalocra nius(Abbott)胚胎发育不同时期及成体肝脏、骨骼肌、肾脏、眼等几种组织器官中LDH同工酶(乳酸脱氢酶同工酶)电泳图谱、EST同工酶(脂酶同工酶)电泳图谱.结果表明:大银鱼LDH有三条酶带;EST有两条酶带.分析表明LDH同工酶、EST同工酶在活力和组织分布方面均具有特异性.     

万寿菊两用系POD同工酶分析及主要观赏性状综合评价

采用聚丙烯酰胺凝胶电泳（PAGE）技术分析了10个万寿菊雄性核不育两用系的POD同工酶,并对其6个主要观赏性状进行了综合评价。结果表明：同系内除小花外不育株与可育株间POD同工酶酶谱差异很小,而系间差异较大;6个主要观赏性状综合表现最优的为4,′其次为3,′最差的为1′。     

过氧化物酶同工酶在南瓜分类中的应用

采用垂直平板聚丙烯酰胺凝胶电泳技术对南瓜属6个不同品种南瓜的过氧化物酶（POD）同工酶进行了分析研究，并采用聚类分析法对其亲缘关系进行比较，结果表明：6个不同品种南瓜根POD共分离出10条酶带，其中E2，E3，E4和E6分离清晰，活性较强，为6个品种所共有。     

四个杂交粳稻的蛋白质、赖氨酸、直链淀粉含量和MDH活性测定

本文分析比较了正在大面积推广种植的杂交粳稻寒优１０２７以及３个新选育的杂交粳稻８优１６１、寒优１９５和寒优３５６的蛋白质含量、赖氨酸含量、赖氨酸相对合量、直挂淀粉含量和苹果田脱氢酶（ＭＤＨ）活性．结果显示了３个新选育的杂交粳稻分别在不同的方面胜过对照寒优１０２７．寒优１９５的直链淀粉合量（１２．１％）明显低于对照寒优１０２７（１７．０％），赖氨酸的相对合量（３．６８％）高于对照寒优１０２７（３．１８％），统计分析两方面的差异都达极著水平（Ｐ＜０．０１）；８优１６１的蛋白质含量（９．６９％）比对照寒优１０２７（８．７８％）高１１．１％，统计分析差异达极显著（Ｐ＜０．０１）；寒优３５６４白质含量（９．１５％）比对照寒优１０２７高．出５．５％，两者差异也达极显著（Ｐ＜０．０１），而且寒优３５６在苗期的ＭＤＨ活性高于对照寒优１０２７，统计分析两者差异显著（Ｐ＜０．０５），这表明寒优３５６在生长后期的每生日穗总粒数，每穗实粒数和每实粒重可能会超过对照寒优１０２７．建议有关部门在今后的水稻生产中，可根据各自不同的需要，推广利用不同的杂交粳稻．     

陈旧性腰椎间盘突出症治疗前后对血清5-HT及5-HIAA的影响

目的：推拿“六法”及硬膜外给药治疗,对陈旧性腰椎间盘突出症患者血清5－HT及5HIAA含量的影响。方法：运用荧光分光光度法测试血清5－HT及5－HIAA含量。结果：治疗后患者血清中5－HT及5－HIAA浓度明显回降。结论：说明上述中西医结合法治疗可以减少5－HT的合成代谢,同时增强了它们的分解代谢。