Effect of temperature on the translocation of bacterial DNA inSolanum lycopersicum esc

Résumé Nous avons étudié l'influence de différentes températures sur la translation de l'ADN d'origine bactérienne chez les plantules de tomate. La température n'a aucune influence en elle-même. Néanmoins des variations de température diminuent la dépolymérisation de l'ADN étranger migré dans la plante.

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This work was done under a Euratom/C.E.N. contract, and was supported by grants from the Belgian Fonds de la Recherche Scientifique Fondamentale Collectye.

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Boursier Euratom.

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Boursier au Fonds national suisse de la recherche scientifique.     

Effects of light on the translocation of bacterial DNA inSolanum lycopersicum esc

Résumé Nous avons étudié l'influence de la lumière sur la translation de l'ADN d'origine bactérienne chez des plantules de tomate. La lumière augmente la prise d'ADN étranger. Cet ADN est, par contre, dans un état moins polymérisé que chez les plantules exposées à l'obscurité. Dans les mêmes conditions expérimentales, la lumière n'a aucune influence sur la synthèse de l'ADN endogène, réalisée à partir de petits précurseurs.     

Transcription of bacterial DNA by isolated plant nuclei.

Plant nuclei prepared from protoplasts can be used as a cell-free system for testing their template activity of procaryotic DNA for plant polymerases. We were able to demonstrate that plant polymerases of Petunia hybrida are capable of transcribing linear bacterial DNA, whereas supercoiled DNA could not be used as a template.     

Effect of sodium chloride on the respiratory function of Staphylococcus aureus.

Sodium chloride at concentrations below 0.5 M, enhanced the respiratory activity (O2-consumption) of Staphylococcus aureus under endogenous and sugar-supported conditions, but did not overcome the inhibitory action of sodium azide. Several sugars, including the glucose analogue alpha-methylglucoside, and their metabolites enhanced bacterial O2-consumption, but acetylmethylcarbinol was ineffective.     

Effect of sodium aurothiomalate ‘Myocrisin’ on DNA synthesis in phytohaemagglutinin-stimulated cultures of sheep lymphocytes

Zusammenfassung Das Antirheumatikum Natrium-Aurothiomalat «Myocrisin» hemmt reversibel die durch Phytohaemagglutinin angeregte Synthese von DNS in Kulturen von Lymphozyten des Schafes.     

Effect of pH on active sodium excretion

Zusammenfassung Die aktive Na-Ausscheidung Nareicher quergestreifter Muskeln konnte durch eine mässige Steigerung der Wasserstoffionekonzentration gehemmt werden. Obgleich der intensivere Kohlehydratstoffwechsel an und für sich die Na-Abgabe steigert, wurde dieser Effekt durch die Herabsetzung des pH unterdrückt.

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On leave from Department of Urology, University of Medicine, Budapest (Hungary).     

Interaction of platinum compounds with bacterial DNA

Summary The cis and trans isomer of PtCl₂(NH₃)₂, cis-Pt(cpa)₂Cl₂ and 2 platinum pyrimidine blues have been used in a number of bacterial tests indicative of their interaction with bacterial DNA.This investigation was supported by a grant from C.N.R., Rome. We thank Mrs S. Saincich for skilful technical assistance.     

Effect of sodium chloride on the respiratory function ofStaphylococcus aureus

Summary Sodium chloride at concentrations below 0.5 M, enhanced the respiratory activity (O₂-consumption) ofStaphylococcus aureus under endogenous and sugar-supported conditions, but did not overcome the inhibitory action of sodium azide. Several sugars, including the glucose analoguea-methylglucoside, and their metabolites enhanced bacterial O₂-consumption, but acetylmethylcarbinol was ineffective.     

Regulatory properties of the citrate synthase from Rhodospirillum rubrum.

Citrate synthase, purified 600-fold from Rhodospirillum rubrum, is activated by KCl and inhibited by ATP and NADH; the effect of the latter inhibitor is completely counteracted by AMP and partially counteracted by KCl.     

DNA and its precursors might interact with the food preservatives,sodium sulphite and sodium benzoate

Summary The interaction of sodium sulphite and sodium benzoate with nucleosides and DNA has been studied in vitro. Reduction in UV-absorbance was consistently noticed. However, no new products result from such interaction. It is likely that our previous observations of the effects of the 2 food preservatives on DNA synthesis and mitotis inVicia faba root meristems is not due to direct action of the chemicals at the level of genetic material.We are grateful to Professor P.A. Robbins and Dr. A.S. Herbin of the Department of Chemistry, University of Nairobi for their help.