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1.
獐毛愈伤组织耐盐性研究及耐盐变异体的筛选   总被引:4,自引:0,他引:4  
獐毛(Aeluropus littoralis var.sinensis)匍匐茎茎尖来源的原始型愈伤组织,测定其耐范围为0.3%~1.5%。盐胁迫下,脯氨酸及K^+、Na^+、Ca^2+离子在渗透调节中起重要作用。用直接筛选的方法,将原始型愈伤组织放在含2%NaCL的选择培养基上连续筛选,获得耐盐性进一步提高的愈伤组织耐盐变异体,在选择培养基上,变异型愈伤组织鲜重相对生长率显大于原始型愈伤组织,  相似文献   

2.
以小麦幼穗和幼胚为外植体,诱导出愈伤组织,通过在培养基中逐渐增加选择剂(NaCl:Na2SO4=4:1)方法,获得耐0.8%至2.0%选择剂的耐盐愈伤组织,其内源脯氨酸含量显著高于相同盐渍条件下的对照组.培养基中选择剂浓度达到1.6%耐盐细胞仍能保持细胞及细胞器正常形态,液泡化程度低,细胞核大,有的细胞中出现二或二个以上核仁.在培养基中选择剂超过1.2%,绝大多数细胞处于坏死或类坏死状态.耐盐愈伤组织在培养基中选择剂不超过2.0%,生长良好,可以再生植株,其后代在0.3%NaCl土壤中仍表现耐盐性.  相似文献   

3.
以小麦幼穗和幼胚为外植体,诱导出愈伤组织,通过在培养基中逐渐增加选择剂(NaCl:Na2SO4=4:1)方法,获得耐0.8%至2.0%选择剂的耐盐愈伤组织,其内源脯氨酸含量显著高于相同盐渍条件下的对照组,培养基中选择剂浓度达到1.6%耐盐细胞仍能保持细胞及细胞器正常形态,液泡化程度低,细胞核大,有的细胞中出现二或二个以上核仁,在培养基中选择剂超过1.2%,绝大多数细胞处于坏死或类坏死状态,耐愈伤组  相似文献   

4.
以糯稻H1701为材料,通过胚培养诱导愈伤组织,继代培养并分化,实验结果表明,NaCl对胚性愈合伤组织的诱导和生长有不同程度的抑制作用,NaCl浓度越高,抑制越明显,转人发化培养基后以后,除含10g/L及以上浓度NaCl的诱导培养基中愈伤组织分化为零外,其余均能分化出苗,其中,含NaCl2g/L的诱导培养基中诱导出愈伤组织分化率最高。  相似文献   

5.
非盐生植物棉花和盐生植物滨藜的盐害机理   总被引:5,自引:0,他引:5  
在短时间(4~7d)内,用等渗的NaCl和NaCl并在根际加压(NaCl+P)分别处理非盐生植物棉花和盐生植物滨藜后,两者幼苗的叶相对扩展率、蒸腾率、木质部汁液透势及叶组织中的K^+含量均降低,而叶细胞质膜透性、木质部汁液和叶组织中Na^+的含量均有增加。NaCl+P处理能减轻等渗NaCl对植物产生的伤害效应,对棉花可减轻约50%,对滨藜减轻程度更大。这说明两种植物所遭受的短期盐害均与离子毒害和渗  相似文献   

6.
NaCl对盐生植物和非盐生植物生理效应的比较   总被引:6,自引:1,他引:6  
研究了不同浓度NaCl对盐生植物碱蓬和非盐生植物大麦幼苗离子含量,甜菜碱水平和甜菜碱醛脱氢酶活性的效应,实验结果表明,不同浓度NaCl处理96h的碱蓬和大麦地上部K^+含量均低于对照,Na^+含量高于对照。  相似文献   

7.
用吸收光谱实验数据和d轨道理论确定了V^2+:NaCl和V^2+:NaBr中的V^2+-Cl^-(Br^-)键长,结果表明,NaCl和NaBr中的V^2+-Cl^-(Br^-)键长明显小于未掺杂晶格中的Na^+-Cl^-(Br^-)离子距离,本文所用的方法不失为一种简单可靠的有效方法。  相似文献   

8.
荔枝(Litchi chinensis)细胞培养的初步研究   总被引:6,自引:0,他引:6  
用荔枝无菌苗作为外植体,在添加了AgNO3呈蜂王浆的含1mg/L6-BA和1mg2,4-D的MS培养基中诱导出愈伤组织,在同样培养基中继代培养,得到了的2细胞系,这种细胞在含1mg/L-6-BA+2mg/L,2,4-D的MS培养基中悬浮培养,培养周期25d,结果表明,维生素C和NaCl在悬浮2中有良好的防褐作用。  相似文献   

9.
通过在培养基逐代增加氯化钠浓度的方法获得耐0.5%、1.0%、1.5%NaCl的红豆草愈伤组织细胞系,并分别继代培养30、25、20次,与非盐适应细胞系的蛋白质电泳图谱相比,其蛋白质SDS-PAGE图谱中出现了83KD、35KD和18KD蛋白质,而非盐适应细胞系中的70KD、37KD、32KD蛋白质则消失或减少。上述83KD、35KD、18KD蛋白质的诱导产生和红豆草愈伤组织在盐环境中表现出的适应  相似文献   

10.
蛇莓果天然色素的稳定性分析   总被引:5,自引:0,他引:5  
研究了常用食品添加剂维生素C,有机酸,糖类和常见和几种金属离子Na^+,K^+,Ca^2+,Mg^2+,Fe^2+,Zn^2+,Al^3+对蛇莓果中天然红色素稳定性的影响。并对色素的耐酸碱性,耐氧化性,耐还原性以及耐热性进行了探讨。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

14.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

15.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

16.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

17.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

18.
For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the  相似文献   

19.
Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of  相似文献   

20.
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with  相似文献   

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