不同光照条件下龙眼体胚发生过程中过氧化物酶同工酶酶谱分析

试验以龙眼胚性愈伤组织为材料,研究不同光照条件对龙眼体细胞胚胎发生各阶段过氧化物酶(POD)同工酶的影响。结果表明:龙眼体细胞胚胎发生各阶段过氧化物酶(POD)同工酶出现了很大的变化。酶带数量总的变化趋势是胚性愈伤组织谱带条数较少,球形胚发生过程中增多,到了子叶形胚后谱带条数又减少;不同光照条件下,蓝光的谱带条数最多,说明不同光照条件对龙眼体细胞胚胎发生有不同效应,意味着蓝光条件下体细胞胚胎内物质代谢是最活跃的,蓝光对体细胞胚胎生长有促进作用。     

刺槐不同愈伤组织的酯酶和过氧化物酶同工酶的差异分析

本研究以刺槐体细胞分化、发育过程中采用的垂直平板聚丙烯酰胺电泳方法分析了不同类型的愈伤组织之间表现出的多态性,结果表明,酯酶同工酶和过氧化物同工酶可用来区分刺槐胚性与非胚性愈伤组织及绿色愈伤组织.     

宁夏枸杞愈伤组织器官发生和体细胞胚发生过程中过氧化物酶和酸性磷酸酶同工酶的研究

利用已建立的宁夏枸杞（Ｌｙｃｉｕｍ ｂａｒｂａｒｕｍ Ｌ．）叶愈伤组织器官发生和体细胞胚发生体系,比较研究了两条离体再生途径早期阶段愈伤组织的生长特性、过氧化物酶（ＰＯＤ）和酸性磷酸酶同工酶的活性及酶谱的变化。结果表明：（１）与继代愈伤组织的“Ｓ”形生长曲线不同,器官发生和体细胞胚发生过程的愈伤组织生长延迟期不明显,且第３ｄ后一直处于指数生长期,培养后期器官发生的愈伤组织生长速率快于体细胞胚发生;     

普通小麦的体细胞胚发生、发育相关蛋白质的毛细管电泳法鉴定

采用高效毛细管电泳技术对普通小麦不同类型的愈伤组织进行蛋白质电泳分析，发现不同类型的愈伤组织中含有多数相同的蛋白质和少数差异蛋白质．结合形态与结构分析，表明差异蛋白质可能与体细胞胚的发生和发育相关．与普通电泳及双向电泳相比，该技术具有快速可靠的特点，可望发展成为鉴定愈伤组织类型及体细胞胚发育时期特异蛋白质的有效方法．     

日本落叶松胚性愈伤组织蛋白组分双向电泳分析

对日本落叶松(Larixleptolepis)胚性愈伤组织进行了组成特点和蛋白质组分双向电泳的研究.研究表明,胚性愈伤组织是由不同发育阶段的早期胚组成的细胞团,早期胚主要由长的胚柄细胞及其顶端的胚头细胞组成,而后者又具不同的分裂相.以三氯乙酸/丙酮法提取胚性组织的总蛋白,通过双向凝胶电泳获得了完整的全蛋白质图谱.运用图像分析软件(I mage Master2D)对电泳图谱分析表明:在凝胶上展现出475个蛋白质组分,并进一步确定每个蛋白质相应的分子质量、等电点和相对含量.从本研究所获得的具高分辨率日本落叶松胚性愈伤组织蛋白质图谱,将为今后与愈伤组织的分化能力相关的蛋白质的检测、分离和基因克隆,以及胚性能力相关蛋白标准化的制定等提供参考.     

芦柑子叶培养中过氧化物酶活性及其同工酶的变化

以芦柑子叶为外植体，对脱分化产生愈伤组织至再分化产生器官的发育过程中过氧化物酶活性及同工酶谱变化的研究结果表明，再分化过程的酶活性高于脱分化过程，根分化过程的酶活性高于芽分化过程。同工酶分析揭示出１０条酶带，其中由子叶经愈伤组织到分化出芽的过程中先后出现了９条酶带，带５（Ｒ＿ｆ＝０．１６７）为这一过程所特有；由子叶经愈伤组织到分化出根的过程中也先后出现了９条酶带，带６（Ｒ＿ｆ＝０．２２５）为该过程所特有。     

水稻悬浮体细胞胚胎发生及其特异蛋白

以水稻优良品种吉农大８号成熟种子为实验材料,建立了悬浮体细胞胚胎发生体系,并获得再生植株,对其发生过程中特异蛋白的变化,以及诱导中胚性与非胚性愈伤组织的差异等问题进行了探讨,为将来深入研究体细胞胚胎发生机理奠定了基础,实验结果表明,不同培养偌上诱导的胚性愈伤组织的发生频率是不同的,基本培养基ＮＭＢ诱导形成的愈伤组织在适当激素浓度下,可由悬浮培养产生体细胞胚,转移到固体培养基上后,得到再生植株,对这一过程进行的组织学观察证实该形态发生途径是单细胞起源的体细胞胚胎发生,利用ＳＤＳ－聚合烯酰胺凝胶电泳比较体细胞胚胎发生中不同时期可溶性蛋白组分的差异,发现相对分子质量为５９０００的蛋白组分存在于分化初期的球形胚中,相对分子质量为５６０００的蛋白组分仅在早期成熟胚中出现;在分化出幼叶的晚期成熟胚中具有相对分子质量为３２０     

外源激素对牡丹胚性愈伤组织诱导的影响

为了探讨外源激素的添加对牡丹胚性愈伤组织诱导过程中生理生化特性的影响.以牡丹品种‘凤丹白’叶柄诱导的愈伤组织为材料,研究不同浓度NAA和TDZ对胚性愈伤组织诱导过程中POD(过氧化物酶)、SOD(超氧化物歧化酶)、CAT(过氧化氢酶)和可溶性蛋白的影响.结果表明,抗氧化酶类活性和蛋白质含量与胚性细胞早期发育发生密切相关,在胚性愈伤组织形成时期,POD和CAT活性变化趋势基本一致,而SOD活性变化则相反;SOD活性经过短暂下降后回升,于第15天达到第二个峰值后稳步下降,表明SOD在胚性愈伤组织诱导初期起主导作用;POD与CAT活性出现峰值时间较SOD滞后,推测POD和CAT活性的升高能够促进胚性愈伤组织的形成和早期发育;可溶性蛋白含量于胚性细胞形成前一直保持较高水平,在第15天达到峰值后下降,说明其含量的升高有利于胚性细胞的形成和发育,其累积对形成胚性愈伤组织有促进作用.添加NAA 0.2 mg/L和TDZ 0.5 mg/L对牡丹胚性愈伤组织的诱导效果最好.     

花生成熟胚叶体细胞胚形成过程中的过氧化物酶同工酶研究

以花生成熟胚的幼叶做外植体诱导体细胞胚发生．从外植体培养到子叶胚形成期间,可溶性蛋白浓度初期下降,以后相对稳定;过氧化物酶活性逐渐升高,过氧化物同工酶谱带由２条陆续增加到１０条,球形胚形成以后同工酶谱带不再变化．     

辣椒不同发育时期的同工酶研究

利用聚丙烯酰胺垂直板电泳技术对5个辣椒品种9个发育期的过氧化物酶同工酶、超氧化物歧化酶同工酶和酯酶同工酶进行了研究．发现不同发育时期3种酶的同工酶酶谱均有不同程度的变化．过氧化物酶同工酶酶谱在五层花序期最丰富,共有11条酶带,而在双叶期和四叶期,未检测到过氧化物同工酶酶带;超氧化物歧化酶酶谱在二层花序期最丰富,共有9条酶带,而在双叶期酶带数最少,仅为3条;酯酶同工酶酶谱在六层花序期最丰富,共有6条酶带,在六叶期和二层花序期酶带数最少,只有1条．通过分析这3种同工酶的总酶谱,找到了区分5个辣椒品种的标志性酶带．     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that