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1.
L W Weber 《Experientia》1987,43(2):176-178
A known ornithine decarboxylase assay working with ion exchange separation of [3H]ornithine and [3H]putrescine has been revised. The assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. The separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. The detection limit is about 50 pmoles [3H]putrescine formed. 相似文献
2.
C. Malo 《Cellular and molecular life sciences : CMLS》1988,44(3):251-252
Summary The Km for ornithine is remarkably constant during the course of postnatal development in both normal and spf mutant mice even if a large but transient increase in ornithine decarboxylase (ODC) activity is noted. Four hours after EGF injection (4 g/g b.wt) to 17-day-old normal and spf mice, a marked stimulation of ODC activity is observed but Km remains unaffected. These data argue against the existence of multiple forms of ODC in the intestinal mucosa of mice.Supported by grant MA-8923 from the Medical Research Council of Canada. The author is Chercheur-boursier du Fonds de la Recherche en Santé du Québec. 相似文献